1.14.13.59: L-lysine N6-monooxygenase (NADPH)

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For detailed information about L-lysine N6-monooxygenase (NADPH), go to the full flat file.

Reaction

Synonyms

EC 1.13.12.10, EC 13.12.10, flavin-dependent lysine monooxygenase, flavin-dependent N6-lysine monooxygenase, IucD, LH, lysine monooxygenase, Lysine N(6)-hydroxylase, Lysine N6-hydroxylase, lysine N6-monooxygenase, lysine: N6-hydroxylase, lysine:N(6)-hydroxylase, Lysine:N6-hydroxylase, MbsG, N-hydroxylating monooxygenase, NbtG, Oxygenase, lysine N6-mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.59 L-lysine N6-monooxygenase (NADPH)

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Results	in table
4	Activating Compound
6102	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
4	General Information
1	General Stability
10	Inhibitors
20	kcat/KM [mM/s]
1	Ki Value [mM]
38	KM Value [mM]
5	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
19	Organism
8	Pathway
4	PDB ID
7	pH Optimum
2	pH Range
25	Protein Variants
4	Purification (Commentary)
5	Reaction
2	Reaction Type
17	Reference
9	Specific Activity [micromol/min/mg]
1	Storage Stability
47	Substrates and Products (Substrate)
5	Subunits
29	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
28	Turnover Number [1/s]

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Cloned on EC 1.14.13.59 - L-lysine N6-monooxygenase (NADPH)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli

Mycolicibacterium smegmatis

-

726965

expression of wild-type and mutants in strain DH5alpha

Escherichia coli

-

659801

gene aerA, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and P14G mutant enzymes in strain M15-2, comparison of nucleotide sequences of enzyme-encoding genes iucD and aerA

Escherichia coli

-

658232

gene iucD, expression of wild-type and mutants in strain DH5alpha

Escherichia coli

-

658171

recombinant expression of His8-MBP-tagged wild-type and mutant K184A enzymes, as well as seleno-L-methionine-labeled enzyme in Escherichia coli TOP10 cells

Nocardia farcinica

-

745322