2.1.1.163: demethylmenaquinone methyltransferase
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For detailed information about demethylmenaquinone methyltransferase, go to the full flat file.
Reaction
Synonyms
2-heptaprenyl-1,4-naphthoquinone methyltransferase, DMK methyltransferase, Dmkt1, DmtH protein, menaquinone biosynthesis methyltransferase, menG, UbiE
ECTree
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General Information on EC 2.1.1.163 - demethylmenaquinone methyltransferase
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GENERAL INFORMATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
evolution
ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes
malfunction
metabolism
physiological function
malfunction
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in the uniE-lacking mutant strain AN70, a lack of ubiquinone occurs due to the missing methylation reaction. The mutant also contains only demethylmenaquinone, no menaquinone. Mutant respiration of DMSO is increased, while the respiration of fumarate is reduced compared to the wild-type enzyme
malfunction
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in the uniE-lacking mutant strain AN70, a lack of ubiquinone occurs due to the missing methylation reaction. The mutant also contains only demethylmenaquinone, no menaquinone. Mutant respiration of DMSO is increased, while the respiration of fumarate is reduced compared to the wild-type enzyme
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metabolism
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the enzyme catalyzes a step in the menaquinone pathway as well as the methylation step in ubiquinone pathway, overview. Menaquinne activates the respiration of fumarate, trimethylamine N-oxide, nitrate, and DMSO
metabolism
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demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R
metabolism
the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis
metabolism
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demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R
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metabolism
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the enzyme catalyzes a step in the menaquinone pathway as well as the methylation step in ubiquinone pathway, overview. Menaquinne activates the respiration of fumarate, trimethylamine N-oxide, nitrate, and DMSO
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physiological function
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mutant defective in the demthylmenaquinone methyltransferase activity expresses the same formate-dependent nitrite reduction activity as the parental strain. Either menaquinone or ubiquinone, but not demethylmenaquinone, can transfer electrons to a third cytochrome-c-dependent electron transfer chain, the periplasmic nitrate reductase
physiological function
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mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
physiological function
strains containing either a disruption or point mutation G142D in ubiE accumulate 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates. Disruption mutants show defects in growth on succinate. The UbiE polypeptide is required for the C methylation reactions in both ubiquinone and menaquinone biosynthesis
physiological function
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the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation
physiological function
expression of MenG is essential. A gradual and partial depletion of MenG over consecutive subcultures results in progressive slowing of growth. Upon MenG depletion, there is a significant accumulation of MenG substrate, demethylmenaquinone, while the cellular level of menaquinone is unaffected. The growth retardation coincides with a lower oxygen consumption rate and ATP accumulation
physiological function
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the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation
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physiological function
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mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
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physiological function
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expression of MenG is essential. A gradual and partial depletion of MenG over consecutive subcultures results in progressive slowing of growth. Upon MenG depletion, there is a significant accumulation of MenG substrate, demethylmenaquinone, while the cellular level of menaquinone is unaffected. The growth retardation coincides with a lower oxygen consumption rate and ATP accumulation
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