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2.1.1.267: flavonoid 3',5'-methyltransferase

This is an abbreviated version!
For detailed information about flavonoid 3',5'-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.267

Reaction

S-adenosyl-L-methionine
+
a 3'-hydroxyflavonoid
=
S-adenosyl-L-homocysteine
 +
a 3'-methoxyflavonoid

Synonyms

3'-OMT, A3'5'OMT, anthocyanin methyltransferase, anthocyanin O-methyltransferase, AnthOMT, AOMT, AOMT1, AOMT2, AOMT3, CdFOMT1, CdFOMT3, CdFOMT4, CdFOMT5, CdFOMT6, CrCOMT2, CrOMT2, EC 2.1.1.149, flavonoid 3',5'-O-dimethyltransferase, flavonoid O-methyltransferase, flavonoid-O-methyltransferase, FOMT1, FOMT3, FOMT4, FOMT5, FOMT6, methyltransferase, flavonoid, Mg2+-independent O-methyltransferase, More, NmAMT6, OMT2, OMT3, PsAOMT, PtAOMT, ROMT-15, ROMT-17, S-adenosylmethionine: anthocyanin 3'5'-methyltransferase, SlOMT3, ThAOMT, TMT5, type II OMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.267 flavonoid 3',5'-methyltransferase

Cloned

Cloned on EC 2.1.1.267 - flavonoid 3',5'-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as glutathione S-transferase fusion protein
expression in Escherichia coli
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gene AOMT, cloned from a cDNA library derived from Torenia hybrida petals that mainly accumulated malvidin type anthocyanins using the petunia A3'OMT cDNA as a probe, phylogenetic analysis and tree. Functional recombinant expression in Nierembergia sp. NB18, the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produce methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin. Transgenic rose petals of Rosa hybrida cv. WKS124 expressing both a pansy flavonoid 3',5'-hydroxylase (F3'5'H) and the Torenia A3'5'OMT genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprise up to 88% of the total anthocyanidins, and their magenta color is more brilliant than that of the petals that accumulate delphinidin type anthocyanins by expressing the F3'5'H gene alone. Agrobacterium tumefaciens strain AGL0 transfection method. Recombinant expression of His-tagged enzyme in Escherichia coli
gene CrOMT2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression as His-tagged enzyme in Escherichia coli
gene FAOMT, expression analysis, MYB diplotypes at the color locus affect anthocyanin composition, relationship analysis between the genotypes of the markers closest to the major QTLs and the expression levels of anthocyanin biosynthetic genes
gene FOMT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene FOMT3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene FOMT4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene FOMT5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of soluble homodimeric His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene FOMT6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene NmAMT6, phylogenetic tree, functional recombinant expression in Escherichia coli
gene OMT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, gene OMT shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes, encoding dihydroflavonol reductase (DFR), flavonoid-3',5'-hydroxylase (F3'5'H), and anthocyanidin synthase (ANS)
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genes AOMT2, AOMT3, and AOMT1, DNA and amino acid sequence determination and analysis, expression analysis, association mapping with VvAOMT1 and VvAOMT2 and methylated anthocyanin profiles, overview
isoform ROMT-15, expression in Escherichia coli
isoform ROMT-17, expression in Escherichia coli
recombinant expression of wild-type anchimeric mutant enzymes in Escherichia coli
sequence comparisons, recombinant expression of MBP-tagged enzyme in Nicotiana tabacum cv. Nc89 leaves and in Fragaria x ananassa cv. Hongyan ripening fruits via transfection with Agrobacterium strain EHA105, quantitative PCR enzyme expression analysis, analysis of flavonoid contents in the transgenic plants
-
sequence comparisons, recombinant expression of MBP-tagged enzyme in Nicotiana tabacum cv. Nc89 leaves and in Fragaria × ananassa cv. Hongyan ripening fruits via transfection with Agrobacterium strain EHA105, quantitative PCR enzyme expression analysis, analysis of flavonoid contents in the transgenic plants
-