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2.3.1.137: carnitine O-octanoyltransferase

This is an abbreviated version!
For detailed information about carnitine O-octanoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.137

Reaction

octanoyl-CoA
+
L-carnitine
=
CoA
 +
L-octanoylcarnitine

Synonyms

carnitine acyltransferase, Carnitine medium-chain acyltransferase, carnitine octanoyltransferase, COT, CRAT, CrOT, easily solubilized mitochondrial carnitine palmitoyltransferase, medium-chain carnitine acyltransferase, medium-chain/long-chain carnitine acyltransferase, More, overt mitochondrial carnitine palmitoyltransferase, short-chain carnitine acyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.137 carnitine O-octanoyltransferase

Cloned

Cloned on EC 2.3.1.137 - carnitine O-octanoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clone from a rat liver library, 2 forms of COT in peroxisomes results of the trans-splicing mechanism in pre-mRNAs
-
cDNA cloning by library screening and recombinant expression in Escherichia coli
-
cDNA PCR amplified, cloned in yeast expression vector pEL26 and expressed in Saccharomyces cerevisiae
cloned and expressed in Escherichia coli
expression in HepG2 cell
expression of wild-type and G553M mutant enzymes in an enzyme-deficient Saccharomyces cerevisiae strain
-
overexpression of the His-tagged enzyme in Escherichia coli as soluble protein, expression of enzyme for selenomethionine-labeling in Escherichia coli strain B834(DE3)
pYES2 plasmid containing the wild-type and the double mutant H131A/H340A of COT expressed in Saccharomyces cerevisiae
-