Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.3.14: homocitrate synthase

This is an abbreviated version!
For detailed information about homocitrate synthase, go to the full flat file.

Word Map on EC 2.3.3.14

Reaction

acetyl-CoA
+
H2O
 +
2-oxoglutarate
=
(2R)-2-hydroxybutane-1,2,4-tricarboxylate
 +
CoA

Synonyms

2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase (CoA-acetylating), acetyl-coenzyme A: 2-ketoglutarate C-transferase, acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase, EC 4.1.3.21, HCS, HcsA, homocitrate synthase, homocitrate synthetase, homocitrate-condensing enzyme, homocondensing enzyme, LYS20, LYS21, Lys21p, LYS22, Lys22p, nifV, nifV2, saci_1304, SpHCS, synthase, homocitrate, TtHCS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.14 homocitrate synthase

Storage Stability

Storage Stability on EC 2.3.3.14 - homocitrate synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -80°C, HEPES buffer with guanidine hydrochloride, alpha-cyclodextrin, ammonium sulfate, KCl and 20% (v/v) glycerol, several months, no significant loss of activity
0-5°C, 0.1 M potassium phosphate buffer, pH 7.8, containing 20% glycerol and 1 mM dithiothreitol, about 50% loss of activity after 2 weeks
-
4-6°C, enzyme in cell-free extract in presence of 20% glycerol, 65% loss of activity after 48 h
-
4°C, buffer without stabilizing components, 72 h, complete loss of activity
4°C, stable for at least 1 week
Saccharomycopsis lipolytica
-