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2.4.1.10: levansucrase

This is an abbreviated version!
For detailed information about levansucrase, go to the full flat file.

Word Map on EC 2.4.1.10

Reaction

sucrose
+
[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
=
D-glucose
 +
[6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside

Synonyms

(2,6)-beta-D-fructan:D-glucose 6-fructosyltransferase, 6-SFT, 6G-FFT2, beta-2,6-fructan: D-glucose-1-fructosyltransferase, beta-2,6-fructan:D-glucose 1-fructosyltransferase, beta-2,6-fructosyltransferase, endolevanase, fructansucrase, fructosyltransferase, sucrose 6-, FTF, Lev, LEV-Y, levanase-sucrase, levansucrase, LevB1, LevB1SacB, LevJ, LEVS, LevU, Lsc, Lsc-3, Lsc2, Lsc3, LscA, LscrA, LSD, LsdA, LvnS, m1ft, M1FT protein, SacB, sucrose 6-fructosyltransferase, sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase, sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase, sucrose:fructan 6-fructosyltransferase, T1-LS, T2-LS, type 1 levansucrase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.10 levansucrase

Specific Activity

Specific Activity on EC 2.4.1.10 - levansucrase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1217
-
sucrose, in culture supernatants
0.13
-
glucose, in culture supernatants
10.8
-
native enzyme from strain type 1, culture supernatant
101.2
crude enzyme, at pH 6.0 and 37°C
10590
-
partially (4.178fold) purified enzyme, at pH 5.2 and 30°C
135.4
-
purified enzyme, pH 6.5, 40°C
152.4
after 1.9fold purification, at pH 6.0 and 37°C
16.82
-
partially purified free enzyme
171
-
levansucrase-ELP[V-40], measured spectrophotometrically
173
-
levansucrase-ELP[V-20], measured spectrophotometrically
18511
-
7.3fold purified enzyme, at pH 5.2 and 30°C
195.3
after 1.93fold purification, at pH 6.0 and 37°C
195.6
-
purified enzyme
2.562
-
glucose, in wild-type strain
203
-
purified enzyme
230
purified recombinant enzyme
2535
-
crude enzyme, at pH 5.2 and 30°C
2561
-
purified periplasmic enzyme
2603
-
purified extracellular enzyme
3.747
-
sucrose, in wild-type strain
37.32
-
purified recombinant His-tagged enzyme from Escherichia coli, release of glucose
415
-
purified enzyme
461.6
purified recombinant wild-type enzyme, pH 6.0, 37°C
470
Aerobacter levanicum
-
purified enzyme
48.19
-
after 5.45fold purification, at pH 5.0 and 40°C
50
-
partially purified enzyme from cell wall
593
-
partially purified native enzyme
669.6
-
recombinant His6-tagged enzyme from Bacillus megaterium, culture supernatant
731
-
purified enzyme
791
purified recombinant His-tagged enzyme, pH 6.5, 50°C, transfructosylation with lactose as acceptor substrate
8.83
-
crude enzyme, at pH 5.0 and 40°C
81.9
crude enzyme, at pH 6.0 and 37°C
90
-
partially purified extracellular enzyme
additional information