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2.4.1.10: levansucrase

This is an abbreviated version!
For detailed information about levansucrase, go to the full flat file.

Word Map on EC 2.4.1.10

Reaction

sucrose
+
[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
=
D-glucose
 +
[6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside

Synonyms

(2,6)-beta-D-fructan:D-glucose 6-fructosyltransferase, 6-SFT, 6G-FFT2, beta-2,6-fructan: D-glucose-1-fructosyltransferase, beta-2,6-fructan:D-glucose 1-fructosyltransferase, beta-2,6-fructosyltransferase, endolevanase, fructansucrase, fructosyltransferase, sucrose 6-, FTF, Lev, LEV-Y, levanase-sucrase, levansucrase, LevB1, LevB1SacB, LevJ, LEVS, LevU, Lsc, Lsc-3, Lsc2, Lsc3, LscA, LscrA, LSD, LsdA, LvnS, m1ft, M1FT protein, SacB, sucrose 6-fructosyltransferase, sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase, sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase, sucrose:fructan 6-fructosyltransferase, T1-LS, T2-LS, type 1 levansucrase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.10 levansucrase

Cloned

Cloned on EC 2.4.1.10 - levansucrase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium is developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase is directed into the growth medium, using the Bacillus megaterium esterase LipA signal peptide
-
affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium
-
cloning and expression of LsdA in a levansucrase deficient Acetobacter diazotrophicus mutant strain
constitutive overexpression in Escherichia coli DH5alpha under control of Rahnella aquatilis levansucrase promotor
Q60114
Escherichia coli strain DH5alpha is used for cloning procedures using a pET3a plasmid and Escherichia coli strain JM109 is used for heterologous protein expression
-
expressed in Escherichia coli BL21 (DE3) Star pLysS cells
expressed in Escherichia coli BL21 Star(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) star pLysS cells
expressed in Escherichia coli Top10 cells
-
expressed in Saccharomyces cerevisiae strain Y2805gs
expression as His-tagged protein in Escherichia coli BL21(DE3), genel evU
Q60114
expression in Acetobacter azotrophicus wild-type strain SRT4, 6fold increase in periplasm, 1.7fold increase of extracellular enzyme
-
expression in Actinomyces naeslundii strain Lev1
-
expression in Escherichia coli
expression in Escherichia coli as His-tagged protein
Q60114
expression in Escherichia coli BL21(DE3) via the T7 RNA polymerase expression system with pET9d
Q60114
expression in Escherichia coli DH5alpha
expression in Escherichia coli JM109
-
expression in Nicotiana tabacum and Cichorium intybus plants
-
expression in Pichia pastoris X-33 from plasmid containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor for entry into the secretory pathway, secretion into the medium
-
expression of His-tagged enzyme in Escherichia coli DH5alpha
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of truncated precursor protein in Pichia pastoris under control of Pichia pastoris-AOX1 promotor, methanol induction, increased thermal stability
-
expression of wild-type and mutant enzymes in Escherichia coli
expression of wild-type and mutants in Escherichia coli
-
gene levU, overexpression of the His-tagged-LevU and of enzyme fused to a chitin-binding domain in Escherichia coli strain DH5alpha
-
gene lsc, two functional alleles lscA and lscB, transcription of lsc is controlled by the hexose metabolism repressor, HexR, since potential HexR binding sites are identified upstream of both lsc genes, analysis of lsc expression by quantitative reverse transcriptase PCR (qRT-PCR)
gene lsc3, expression in Escherichia coli
gene lsc3, sequence comparison, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene lscA, expression in Escherichia coli DH5alpha
-
gene lsdA, organized in an operon with exolevanase LsdB, transcriptional linkage of the chromosomal lsdAB gene cluster
gene mlft, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene sacB from mutant strain QB252, DNA sequencing reveals an amino acid exchange R331H, expression of mutants in Escherichia coli strain XL-1 B
-
gene sacB, cloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3), and high xylose-inducible expression of the His6-tagged enzyme in Bacillus megaterium, where the recombinant enzyme is secreted to the medium, method optimization by response-surface methodology, overview. The enhanced secretion of His6-tagged protein is an advantage for purification via the C-terminal His6 tag, which leads to the purification of intact levansucrase, and a 21fold higher compared to the untagged enzyme
-
gene sacB, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli
-
gene sacB, expressed in Escherichia coli strain SK1592, precursor form
-
gene sacB, overexpression in Bacillus subtilis mutant degU32(hy), deficient in secA translocase, enzyme protein content is directly correlated with translocase content, decrease of translocase leads to accumulation of unprocessed levansucrase precursor and vice versa
-
insertion of Shine Dalgarno like sequences in the 5'-untranslated sacR region controlling the expression of sacB. Depending on the number of stabilizing sequences inserted and the position of these sequences with respect to the translation start codon, it is observed that the mRNA stability and the final protein production could be increased or decreased
-
overexpression as His-tagged protein in Escherichia coli strain His-Is M15 and DH10B, partial gene ftf without the predicted signal sequence
-
overexpression of His-tagged enzyme in Escherichia coli, gene lscR
-
recobinant expression of N-terminally His6- and GST-tagged enzyme in Escherichia coli
recombinant expression in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli
-
recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3)
screening of barley cDNA library, cloning, transient functional expression in Nicotiana plumbaginifolia protoplasts under control of cauliflower mosaic virus 35S promotor
-
truncated levansucrase gene lacking the signal peptide is expressed in Escherichia coli BL21 cells
wild-type enzyme and 16 variants are expressed in Escherichia coli
-