2.4.1.100: 2,1-fructan:2,1-fructan 1-fructosyltransferase

This is an abbreviated version!
For detailed information about 2,1-fructan:2,1-fructan 1-fructosyltransferase, go to the full flat file.

Reaction

Synonyms

1,2-beta-D-fructan:1,2-beta-D-fructan 1F-beta-D-fructosyltransferase, 1,2-beta-fructan 1F-fructosyltransferase, 1-FFT, 6G-FFT/1-FFT, FFT, fructan: fructan 1-fructosyltransferase, fructan:fructan 1-fructosyl transferase, fructan:fructan 1-fructosyltransferase, fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase, fructan:fructan fructosyl transferase, fructan:fructan-1-fructosyltransferase, fructosyltransferase, 1,2-beta-D-fructan 1F-, high DP 1-FFT, high DP fructan:fructan 1-fructosyl transferase, Wft3

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.100 2,1-fructan:2,1-fructan 1-fructosyltransferase

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Results	in table
3	Activating Compound
17	AA Sequence
7	Application
1	CAS Registry Number
12	Cloned(Commentary)
9	Expression
9	General Information
1	General Stability
16	Inhibitors
1	Ki Value [mM]
11	KM Value [mM]
2	Localization
10	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
47	Organism
1	Pathway
9	pH Optimum
1	pH Range
1	pH Stability
3	Posttranslational Modification
9	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
46	Reference
36	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
87	Substrates and Products (Substrate)
8	Subunits
52	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]

Purification

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Purification on EC 2.4.1.100 - 2,1-fructan:2,1-fructan 1-fructosyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

7 entries

ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast’s expression medium

Lolium perenne

Q8S337

706297

Fast Desalting column chromatography

Triticum aestivum

Q4AEI9

704883

-

Triticum aestivum

-

488349

-

Helianthus tuberosus

-

488352, 488358

-

Allium cepa

-

488346, 660021

-

Taraxacum officinale

-

488348

-

Cichorium intybus

-

488353, 488357

-

Lolium rigidum

-

488350, 488351

-

Echinops ritro

Q0PCC9

675149