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0.051
-
be1-1 be3-2, T-DNA insertion double mutant in enzyme BE1 and BE3, in vitro assay of starch-branching enzyme activity
0.082
-
be3-1 (N548089), T-DNA insertion mutant line for BE3, in vitro assay of starch-branching enzyme activity
0.096
-
be1-1 (DYK140), T-DNA insertion mutant line for BE1, in vitro assay of starch-branching enzyme activity
0.102
-
be3-2 (EQJ13), T-DNA insertion mutant line for BE3, in vitro assay of starch-branching enzyme activity
0.13
-
be1-2 (N637880), T-DNA insertion mutant line for BE1, in vitro assay of starch-branching enzyme activity
0.281
mutant enzyme I571D, pH and temperature not specified in the publication
0.282
wild type enzyme, pH and temperature not specified in the publication
0.293
mutant enzyme T339E, pH and temperature not specified in the publication
0.295
mutant enzyme Q231R, pH and temperature not specified in the publication
0.296
mutant enzyme T339D, pH and temperature not specified in the publication
0.298
mutant enzyme Q231K, pH and temperature not specified in the publication
10.7
purified recombinant enzyme, pH 7.5, 25°C, substrate amylose
26
mutant enzyme A310G, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
28.5
-
mutant enzyme DELTA1-63, micromol Glc incorporated into alpha-D-glucan per min at 30°C
30
mutant enzyme A310I, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
384
wild type enzyme, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
4.7
wild-type enzyme, branching assay
42
mutant lacking the N1 domain, i.e. lacking N-terminal residues 1-108, pH 7.0, 25°C
47.3
Cyanobacterium sp.
purified recombinant His-tagged isozyme BE1, pH 7.0, 30°C
535.4
-
mutant enzyme DELTA1-83, micromol Glc incorporated into alpha-D-glucan per min at 30°C
54
mutant enzyme A310N, using potato amylose type III as substrate, at pH 7.5 and 37°C
58
mutant enzyme A310G, using potato amylose type III as substrate, at pH 7.5 and 37°C
63.75
wild-type, pH 7.0, 25°C
67
mutant enzyme A310E, using potato amylose type III as substrate, at pH 7.5 and 37°C
7.2
wild-type enzyme, branching assay
70
mutant enzyme A310D, using potato amylose type III as substrate, at pH 7.5 and 37°C
90.28
-
purified recombinnat enzyme, pH 8.5, 55°C, substrate amylose
951.4
Cyanobacterium sp.
purified recombinant His-tagged isozyme BE1, pH 7.0, 30°C
988.8
Cyanobacterium sp.
purified recombinant His-tagged isozyme BE1, pH 7.0, 30°C
0.001
-
be1-1 be2-1, T-DNA insertion double mutant in enzyme BE1 and BE2, in vitro assay of starch-branching enzyme activity
0.001
-
be2-2 (DSA16), T-DNA insertion mutant line for BE2, in vitro assay of starch-branching enzyme activity
0.002
-
be2-1 (EFH20), T-DNA insertion mutant line for BE2, in vitro assay of starch-branching enzyme activity
0.002
-
be2-1 be3-2, T-DNA insertion double mutant in enzyme BE2 and BE3, in vitro assay of starch-branching enzyme activity
0.107
-
wild type enzyme, Col-0
0.107
-
wild type enzyme, Wassilewskija genetic background
27
mutant enzyme A310N, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
27
mutant enzyme A310Q, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
299
-
branching enzyme II
299
wild type enzyme, using potato amylose type III as substrate, at pH 7.5 and 37°C
32
mutant enzyme A310D, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
32
mutant enzyme A310E, using potato amylopectin type III as substrate, at pH 7.5 and 37°C
62
mutant enzyme A310I, using potato amylose type III as substrate, at pH 7.5 and 37°C
62
mutant enzyme A310Q, using potato amylose type III as substrate, at pH 7.5 and 37°C
additional information
the branching enzyme has a spesific activity of 0.4 U/mg. Amylose is used as substrate. The methods defines one unit of branching enzyme activity as 1 micromol of alpha-1,6 linkages synthesized per minute.
additional information
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the branching enzyme has a spesific activity of 0.4 U/mg. Amylose is used as substrate. The methods defines one unit of branching enzyme activity as 1 micromol of alpha-1,6 linkages synthesized per minute.
additional information
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additional information
specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay
additional information
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specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay
additional information
specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay
additional information
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specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay
additional information
specific activity on amylopectin 474.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay
additional information
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specific activity on amylopectin 474.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay
additional information
specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay
additional information
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specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay
additional information
specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay
additional information
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specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay
additional information
specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay
additional information
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specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay
additional information
specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay
additional information
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specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay
additional information
specific activity on amylose 621.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay
additional information
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specific activity on amylose 621.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay
additional information
specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay
additional information
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specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay
additional information
specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay
additional information
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specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay
additional information
specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay
additional information
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specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay
additional information
specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay
additional information
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specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay
additional information
specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay
additional information
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specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay
additional information
specific activity on amylopectin 538.0 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay
additional information
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specific activity on amylopectin 538.0 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay
additional information
specific activity on amylopectin 556.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant CTT Dr, iodine assay
additional information
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specific activity on amylopectin 556.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant CTT Dr, iodine assay
additional information
specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay
additional information
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specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay
additional information
specific activity on amylose 404.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay
additional information
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specific activity on amylose 404.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay
additional information
specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay
additional information
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specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay
additional information
specific activity on amylose 442.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant CTT Dr, iodine assay
additional information
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specific activity on amylose 442.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant CTT Dr, iodine assay
additional information
specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay
additional information
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specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay
additional information
specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay
additional information
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specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay
additional information
specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay
additional information
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specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay
additional information
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additional information
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additional information
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assay method
additional information
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1.229 U/mg for amylopectin, 1 U = 1% decrease of the absorbance/min
additional information
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12.258 U/mg for amylose, 1 U = 1% decrease of the absorbance/min
additional information
2.5 U/mg for amylopectin, 1 U = decrease of one absorbance unit at 660 nm at 30°C
additional information
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2.5 U/mg for amylopectin, 1 U = decrease of one absorbance unit at 660 nm at 30°C
additional information
20.8 U/mg for amylose, 1 U = decrease of one absorbance unit at 660 nm at 30°C
additional information
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20.8 U/mg for amylose, 1 U = decrease of one absorbance unit at 660 nm at 30°C
additional information
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the chimeric (1Na/2Nb)II enzyme has a specific activity of 13 U/mg in the presence of 0.1 M citrate or 6.1 U/mg in the absence of citrate. The iodine-staining assay is performed by monitoring the decrease in absorbance at 660 nm for amylose, one unit of enzyme activity is defined as the amount of enzyme yielding a decrease in A660 of 0.1 per minute at 30°C.
additional information
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0.29 U/mg for the crude cell extract, 1 U = change in the absorbance of 1 unit
additional information
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1.00 U/mg for the purified enzyme , 1 U = change in the absorbance of 1 unit
additional information
0.252 U/mg for the crude enzyme, 1 U = decrease of 1.0 units of absorbance per minute
additional information
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0.252 U/mg for the crude enzyme, 1 U = decrease of 1.0 units of absorbance per minute
additional information
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186 U/mg after ammonium sulfate fractionation, 1 unit = incorporation of 1 nmol glucose into the ethanol-insoluble glucan polymer per minute
additional information
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39 U/mg for crude extract, 1 unit = incorporation of 1 nmol glucose into the ethanol-insoluble glucan polymer per minute
additional information
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483 U/mg after DEAE fast flow, 1 unit = incorporation of 1 nmol glucose into the ethanol-insoluble glucan polymer per minute
additional information
6.402 U/mg for the purified enzyme, 1 U = decrease of 1.0 units of absorbance per minute
additional information
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6.402 U/mg for the purified enzyme, 1 U = decrease of 1.0 units of absorbance per minute
additional information
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744 to 4001 U/mg for enzyme after purification, 1 unit = incorporation of 1 nmol glucose into the ethanol-insoluble glucan polymer per minute
additional information
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assay method