Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.21: starch synthase (glycosyl-transferring)

This is an abbreviated version!
For detailed information about starch synthase (glycosyl-transferring), go to the full flat file.

Word Map on EC 2.4.1.21

Reaction

ADP-alpha-D-glucose
+
[(1->4)-alpha-D-glucosyl]n
=
ADP
 +
[(1->4)-alpha-D-glucosyl]n+1

Synonyms

adenosine diphosphate glucose-starch glucosyltransferase, adenosine diphosphoglucose-starch glucosyltransferase, ADP-glucose starch synthase, ADP-glucose synthase, ADP-glucose transglucosylase, ADP-glucose-starch glucosyltransferase, ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase, ADPG starch synthetase, ADPG-starch glucosyltransferase, AtSS2, AtSSII, AtSSIII, CkGBSS, dull1, GBSS, GBSSI, glgA, glucosyltransferase, adenosine diphosphoglucose-starch, glycogen synthase, glycogen/starch synthase, granule bound starch synthase, granule-bound starch synthase, granule-bound starch synthase I, GSase, OsGBSSII, OsSSIII-1, OsSSIII-2, OsSSIV-1, OsSSIV-2, PvSSI, PvSSII-1, SBD123, SS1, SS4, SSI, SSII, SSII(a), SSIIa, SSIIc, SSIII, SSIII(a), SSIII-1, SSIII-B, SSIII-C, SSIIIa, SSIIIb, SSIV, SSS, SSSI, SSV, starch synthase, starch synthase 1, starch synthase 4, starch synthase I, starch synthase II, starch synthase IIa, starch synthase III, starch synthase III-1, starch synthase III-2, starch synthase III-B, starch synthase IIIa, starch synthase IV, starch synthase IV-1, starch synthase IV-2, starch synthetase, starch-synthase III, starch/glycogen synthase, TaSSIVb, TVAG_258220

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.21 starch synthase (glycosyl-transferring)

Crystallization

Crystallization on EC 2.4.1.21 - starch synthase (glycosyl-transferring)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 0.2 MLi2SO4, 0.1MBis-Tris pH 5.5, 25% (w/v) PEG3350
catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 2 M (NH4)2SO4, 2% (w/v) PEG400 and 150 mM HEPES pH 7.5
cocrystallization of the inactive glycogen synthase mutant E377A with substrate ADPGlc and cocrystallization of wild-type glycogen synthase with substrate ADPGlc and the glucan acceptor mimic 4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane)sulfonic acid, i.e. HEPPSO produces a closed form of glycogen synthase and suggests that domain-domain closure accompanies glycogen synthesis. Four bound oligosaccharides are observed, G6a in the interdomain cleft and G6b, G6c, and G6d on the N-terminal domain surface. Extending from the center of the enzyme to the interdomain cleft opening, G6a mostly interacts with the highly conserved N-terminal domain residues lining the cleft of glycogen synthase. The surface-bound oligosaccharides G6c and G6d have less interaction with enzyme and exhibit a more curled, helixlike structural arrangement
structure of the wild-type enzyme bound to ADP and glucose reveals a 15.2° overall domain-domain closure. The main chain carbonyl group of His-161, Arg-300, and Lys-305 are suggested to act as critical catalytic residues in the transglycosylation. Glu-377 is found on the-face of the glucose and plays an electrostatic role in the active site and as a glucose ring locator. In the mutant E377A-ADP-4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane)sulfonic acid complex the glucose moiety is either absent or disordered in the active site
sitting drop vapor diffusion method, using 100 mM sodium cacodylate buffer pH 6.5, 17% (w/v)v PEG 8K, 0.2 M ammonium sulfate