2.4.1.248: cycloisomaltooligosaccharide glucanotransferase
This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.
Word Map on EC 2.4.1.248
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2.4.1.248
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dextran
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circulans
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glycoside
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polymerization
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cyclization
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carbohydrate-binding
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isomaltooligosaccharides
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paenibacillus
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transglucosylation
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starch
-
synthesis
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subsite
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alpha-1,6-linkage
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maltooligosaccharides
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isomaltotetraose
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transglutaminase
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dextranolytic
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amylose
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hollow-fiber
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isomaltose
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dextranases
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thermoanaerobacter
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porous
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sugar-binding
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disproportionation
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multilayer
-
mutans
- 2.4.1.248
- dextran
- circulans
- glycoside
- polymerization
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cyclization
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carbohydrate-binding
- isomaltooligosaccharides
- paenibacillus
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transglucosylation
- starch
- synthesis
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subsite
-
alpha-1,6-linkage
- maltooligosaccharides
- isomaltotetraose
- transglutaminase
-
dextranolytic
- amylose
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hollow-fiber
- isomaltose
- dextranases
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thermoanaerobacter
-
porous
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sugar-binding
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disproportionation
-
multilayer
- mutans
Reaction
cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =
Synonyms
cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase
ECTree
2.4.1.248 cycloisomaltooligosaccharide glucanotransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
amylose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
glycogen
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 14.6%
-
?
isomaltopentaose
?
WP_028992696
the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates
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-
?
maltodextrin
?
WP_028992696
the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates
-
-
?
maltoheptaose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 15.8%
-
?
maltohexaose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 12.7%
-
?
maltopentaose
?
WP_028992696
the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates
-
-
?
maltopentaose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 8.9%
-
?
maltotetraose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 4.3%
-
?
maltotriose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
poor substrate
yield 0.9%
-
?
partially hydrolyzed starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
starch
?
key enzyme to synthesize alpha-(1->6)-glucan for cycloisomaltooligosaccharide production in dextran-free environments
-
-
?
starch
cycloisomaltooligosaccharide
the enzyme has a strong alpha-(1->4) to alpha-(1->6) transglucosylation activity. The enzyme exhibits higher hydrolysis activity against alpha-(1->4)-linked glucan than against alpha-(1->6)-linked glucan. It elongates alpha-(1->6)-linked glucooligosaccharide to at least a degree of polymerization of 10 through a successive transglucosylation reaction
-
-
?
amylose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 27.2%
-
?
amylose
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 27.2%
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?
dextran
?
-
-
synthesis of seven- to nine-glucose-membered cycloisomaltooligosaccharides
-
?
dextran
?
Niallia circulans T-3040
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-
synthesis of seven- to nine-glucose-membered cycloisomaltooligosaccharides
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?
dextran
?
WP_028992696
cycloisomaltooligosaccharide-7 to cycloisomaltooligosaccharide-20 are mainly produced, with CI-7 as the main product
-
-
?
partially hydrolyzed starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
i.e. Pinedex no. 1, dextrose equivalent
yield 13.9%
-
?
partially hydrolyzed starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
i.e. Pinedex no. 100, dextrose equivalent 2 to 5
yield 201%
-
?
partially hydrolyzed starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
i.e. Pinedex no. 1, dextrose equivalent
yield 13.9%
-
?
partially hydrolyzed starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
i.e. Pinedex no. 100, dextrose equivalent 2 to 5
yield 201%
-
?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
-
?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 25.9%, optimization of production parameters
-
?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 26.5%
-
?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
-
-
yield 25.9%, optimization of production parameters
-
?
soluble starch
cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ?
Niallia circulans T-3040
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-
-
?
additional information
?
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-
enzyme acts on maltooligosaccharides that have degrees of polymerization of 3 or more, amylose, and soluble starch, to produce cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) by an intramolecular alpha-1,6-glycosyl transfer reaction. Enzyme additionally catalyzes the transfer of part of a linear oligosaccharide to another oligosaccharide by an intermolecular alpha-1,4-glycosyl transfer reaction
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-
?
additional information
?
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CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran
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-
?
additional information
?
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isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose
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-
?
additional information
?
-
carbohydrate-binding module CBM35 functions in determining the size of the product, causing the predominant production of cycloisomaltooctaose by the enzyme. The isomaltoheptaose and cycloisomaltooctaose bind in the catalytic cleft with a circular structure around Met-310, representing the enzyme-product complex. The canonical sugar-binding site of CBM35 binds the mid-part of isomaltooligosaccharides
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-
?
additional information
?
-
CITase from Bacillus circulans T-3040 produces mainly cycloisomaltooligosaccharides of 8 units with little megalo-cycloisomaltooligosaccharides with 10-12 glucose units
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-
?
additional information
?
-
Niallia circulans T-3040
CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran
-
-
?
additional information
?
-
Niallia circulans T-3040
CITase from Bacillus circulans T-3040 produces mainly cycloisomaltooligosaccharides of 8 units with little megalo-cycloisomaltooligosaccharides with 10-12 glucose units
-
-
?
additional information
?
-
Niallia circulans T-3040
carbohydrate-binding module CBM35 functions in determining the size of the product, causing the predominant production of cycloisomaltooctaose by the enzyme. The isomaltoheptaose and cycloisomaltooctaose bind in the catalytic cleft with a circular structure around Met-310, representing the enzyme-product complex. The canonical sugar-binding site of CBM35 binds the mid-part of isomaltooligosaccharides
-
-
?
additional information
?
-
Niallia circulans T-3040
isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose
-
-
?
additional information
?
-
isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-598K is cycloisomaltoheptaose
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-
?
additional information
?
-
the enzyme displays extremely low activity against 4-nitrophenyl alpha-glucopyranoside. It does not display any hydrolytic activity against 4-nitrophenyl alpha-mannopyranoside, 4-nitrophenyl alpha-galactopyranoside, and 4-nitrophenyl alpha-xylopyranoside
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