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2.4.1.4: amylosucrase

This is an abbreviated version!
For detailed information about amylosucrase, go to the full flat file.

Word Map on EC 2.4.1.4

Reaction

sucrose
+
[(1->4)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->4)-alpha-D-glucosyl]n+1

Synonyms

AaAS, ACAS, AmAS, AMS, Amy-1, ASASE, BtAS, CcAS, DGAS, DRAS, DRpAS, glucosyltransferase, sucrose-1,4-alpha-glucan, MaAS, MFAS, More, NPAS, NsAS, sucrose-glucan glucosyltransferase, SyAS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.4 amylosucrase

Cloned

Cloned on EC 2.4.1.4 - amylosucrase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into an inducible expression system in Escherichia coli
codon-optimized and expressed in Escherichia coli BL21 (DE3)
DNA library construction and DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Eschetrichia coli
D3A730
expression inn Escherichia coli
expression of GST-tagged ASase in Escherichia coli strain BL21 (DE3)
-
expression of GST-tagged DgAS
expression of GST-tagged DGAS in Escherichia coli strain BL21
-
expression of His-tagged NPAS in Escherichia coli strain BL21
expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
expression of wild-type and random mutants in Escherichia coli strain JM109
gene acas, locus CP001341, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme
-
gene ams, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His6-tagged in Escherichia coli strain Rosettta (DE3)
gene ams, recombinant expression as starch-binding domain-fusion protein in Solanum tuberosum plants, cv. Kardal and amf, using the Agrobacterium tumefaciens transformation method
gene amsA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLyS
gene dgas, DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain JM109
-
gene dgas, expression of GST-tagged DGAS in Escherichia coli
gene dgas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene drpas, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of the His-tagged enzyme in Escherichia coli
-
gene mfas, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene npas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum
His6-tagged enzyme expression in Escherichia coli strain MC1061 using a constitutive expression system
-
overexpressed in Escherchia coli
potential use for the synthesis or the modification of polysaccharides is limited by its low catalytic efficiency on sucrose alone, its low stability, and its side reactions resulting in sucrose isomer formation. Development of a zero background expression cloning strategy for the generation of large variant libraries, a selection mechanism to discard inactive variants, and a screening method for identification of interesting clones
-
recombinant expression in Escherichia coli strain BL21
-
recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-