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2.7.1.59: N-acetylglucosamine kinase

This is an abbreviated version!
For detailed information about N-acetylglucosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.59

Reaction

ATP
+
N-acetyl-D-glucosamine
=
ADP
 +
N-acetyl-D-glucosamine 6-phosphate

Synonyms

2-acetylamino-2-deoxy-D-glucose kinase, acetylaminodeoxyglucokinase, acetylglucosamine kinase(phosphorylating), ATP:2-acetylamino-2-deoxy-D-glucose 6-phosphotransferase, CaNag5p, GlcNAc kinase, GlcNAc-kinase, GNK, Hxk1, MurK, N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, N-acetylmuramic acid/N-acetylglucosamine kinase, NAG5, NAGA kinase, NagK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.59 N-acetylglucosamine kinase

Cloned

Cloned on EC 2.7.1.59 - N-acetylglucosamine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6 point mutations in cysteine residues, expression in Escherichia coli BL21
-
DNA and amino acid sequence determination and analysis, overexpression in strain XL1-Blue
-
expressed in Escherichia coli BL21 (DE3)-pLysS cells
-
expressed in Escherichia coli Bl21(DE3) cells and in a Saccharomyces cerevisiae hxk1 hxk2 glk1 triple mutant
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli strain BL21 (DE3)
expressed in HEK-293T cells
-
expression in Saccharomyces cerevisiae strain BY4734 from the yeast PGK1 promoter leading to incorporation of exogenous GlcNAc into chitin in engineered Saccharomyces cerevisiae, which is devoid of GlcNAc kinase
Q59RG5
expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
functionally expressed in Escherichia coli as glutathione-S-transferase fusion protein, active enzyme
functionally expressed in Escherichia coli BL21, active enzyme, high rate of protein expression in transfected cells, inclusion bodies
gene hxk1, expression of GFP-tagged wild-type enzyme in enzyme-deficient disruption mutant Candida albicans strain CAI4 and expression of TAP- and His-tagged HXK1 under ADH1 p in CAI-4, co-expression with histone deacetylase SIR2
-
GST fusion protein, expressed in Escherichia coli