2.7.1.67: 1-phosphatidylinositol 4-kinase

This is an abbreviated version!
For detailed information about 1-phosphatidylinositol 4-kinase, go to the full flat file.

Reaction

Synonyms

1-phosphatidyl-1D-myo-inositol 4-phosphotransferase, 56 kDa type II PtdIns 4-kinase, alpha type II PI 4-kinase, AtPI4KIIIalpha1, AtPI4KIIIbeta1, AtPI4KIIIbeta2, beta type II PI 4-kinase, beta-type phosphatidylinositol 4-kinase, kinase, phosphatidylinositol (phosphorylating), Lsb6, Lsb6p, phosphatidyl-inositol 4-kinase type IIIalpha, phosphatidylinositol 4 kinase, phosphatidylinositol 4 kinase IIIbeta, phosphatidylinositol 4-kinase, phosphatidylinositol 4-kinase beta, phosphatidylinositol 4-kinase beta1, phosphatidylinositol 4-kinase IIalpha, phosphatidylinositol 4-kinase IIbeta, phosphatidylinositol 4-kinase III alpha, phosphatidylinositol 4-kinase IIIalpha, phosphatidylinositol 4-kinase IIIbeta, phosphatidylinositol 4-kinase IIIbeta,, phosphatidylinositol 4-kinase type 2-alpha, phosphatidylinositol 4-kinase type II, phosphatidylinositol 4-kinase type II alpha, phosphatidylinositol 4-kinase type IIalpha, phosphatidylinositol 4-kinase type IIbeta, phosphatidylinositol 4-kinase type-III alpha, phosphatidylinositol kinase, phosphatidylinositol-4-kinase, phosphatidylinositol-4-kinase IIalpha, phosphatidylinositol-4-kinase III beta, phosphatidylinositol-4-kinase type II alpha, phosphatidylinositol-4-kinase type IIalpha, PI 4-kinase, PI 4-kinase type IIalpha, PI kinase, PI-4 kinase, PI4-kinase, PI4K, PI4K beta, PI4K IIalpha, PI4K IIbeta, PI4K IIIbeta, PI4K2, PI4K230, PI4K2A, PI4K55, PI4K92, PI4KA, PI4KB, PI4Kbeta, PI4Kbeta1, PI4Kgamma3, PI4Kgamma5, PI4KII, PI4KIIalpha, PI4KIIbeta, PI4KIII-beta, PI4KIIIalpha, PI4KIIIbeta, Pik1, Pik1p, PIK230, Pik2p, Pik4ca, PIK4IIalpha, PIK4IIbeta, PIK4IIIalpha, PIK4IIIbeta, PIK55, PIKCB, PtdIns 4-kinase, PtdIns 4-kinase beta, PtdIns4KIIalpha, Stt4p, type II phosphatidylinositol 4-kinase, type II phosphatidylinositol kinase, type II phosphoinositide 4-kinase, type II PI 4-kinase enzyme, type II PI4Kalpha, type II PtdIns 4-kinase, type IIalpha phosphatidylinositol 4-kinase, type III alpha phosphatidylinositol 4-kinase, type III phosphatidylinositol 4-kinase alpha, type III phosphatidylinositol 4-kinase beta, type III PI 4-kinase beta, type III PI4K, type III PI4Kbeta, type-II PI4K, type-III phosphatidylinositol 4-kinase beta1, type-III phosphatidylinositol 4-kinase beta2, type-III PI4K

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.67 1-phosphatidylinositol 4-kinase

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Results	in table
70	Activating Compound
10362	AA Sequence
3	Application
1	CAS Registry Number
33	Cloned(Commentary)
31	Cofactor
5	Crystallization (Commentary)
330	Disease/ Diagnostics
3	Expression
63	General Information
1	General Stability
160	IC50 Value
470	Inhibitors
42	Ki Value [mM]
72	KM Value [mM]
166	Localization
74	Metals/Ions
49	Molecular Weight [Da]
71	Natural Substrates/ Products (Substrates)
155	Organism
4	Pathway
49	PDB ID
27	pH Optimum
14	pH Range
1	pH Stability
1	pI Value
10	Posttranslational Modification
26	Protein Variants
47	Purification (Commentary)
5	Reaction
1	Reaction Type
144	Reference
1	Renatured (Commentary)
131	Source Tissue
17	Specific Activity [micromol/min/mg]
7	Storage Stability
164	Substrates and Products (Substrate)
29	Subunits
242	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
9	Temperature Stability [°C]
4	Turnover Number [1/s]

Engineering

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Engineering on EC 2.7.1.67 - 1-phosphatidylinositol 4-kinase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D1899A

Homo sapiens

-

mutant of isozyme PI4KA

723544

D308A

Homo sapiens

-

enzymatically inactive isozyme PI4KIIalpha

722121

K165-K172

Homo sapiens

Q9BTU6

when the entire membrane binding segment K165-K172 was mutated to alanines, the kinase is inactive

738109

K1792L

Homo sapiens

-

site-direcetd mutagenesis, inactive isozyme PI4KA

723072

N163A

Homo sapiens

Q9BTU6

the mutant shows about 10% of wild type activity

738109

N249A

Homo sapiens

Q9BTU6

the mutant shows slightly reduced wild type activity

738109

PI4K230 DELTA1-1189

Homo sapiens

-

deletion mutant with 97000 Da compared to wild-type enzyme of 230000 Da, the enzyme is stable but activity is not detectable

641806

PI4K230 DELTA1-1427

Homo sapiens

-

deletion mutant with 68000 Da compared to wild-type enzyme of 230000 Da, the enzyme is stable but activity is not detectable

641806

PI4K230 DELTA1-1531

Homo sapiens

-

deletion mutant with 56000 Da compared to wild-type enzyme of 230000 Da, the enzyme is stable but activity is not detectable

641806

PI4K230 DELTA1-872

Homo sapiens

-

deletion mutant with 130000 Da compared to wild-type enzyme of 230000 Da, the enzyme is stable, 0.048 mM/mg*min compared to 0.058 mM/mg*min for the wild-type enzyme

641806

R275A

Homo sapiens

Q9BTU6

the mutant shows about 15% of wild type activity

738109

R275W

Homo sapiens

-

the mutation is associated with inherited cutis laxa and leads to a large reduction in enzyme activity

759586

V339A

Homo sapiens

Q9BTU6

the mutant shows slightly reduced wild type activity

738109

H1484Y

Plasmodium falciparum

Q8I406

the mutant shows reduced activity compared to the wild type enzyme

758830

S1320L

Plasmodium falciparum

Q8I406

the mutant shows reduced activity compared to the wild type enzyme

758830

Y1356F

Plasmodium falciparum

Q8I406

the mutant shows reduced activity compared to the wild type enzyme

758830

D387A

Saccharomyces cerevisiae

-

no activity

674999

D413A

Saccharomyces cerevisiae

-

no activity

674999

K192M

Saccharomyces cerevisiae

-

no activity

674999

N392A

Saccharomyces cerevisiae

-

no activity

674999

additional information

6 entries

additional information

Bos taurus

-

creation of a glutathione S-transferase PI4Kbeta fusion protein that is biologically active and phosphorylates phosphatidylinositol in ist 4-position with wortmannin sensitivity and kinetic parameters that are identical to those of purified wild-type enzyme

641810

additional information

Homo sapiens

-

Huh7.5.1 cells are transduced with a nontargeting lentiviral shRNA construct or shRNA constructs targeting PI4KA or PI4KB. Expression of shRNA-resistant wild-type PI4KA rescues HCV replication in OR6 cells silenced for endogenous PI4KA, while the kinase-dead PI4KA D1899A mutant cannot rescue viral replication in OR6 cells silenced for endogenous PI4KA

723544

additional information

Homo sapiens

-

isozyme PI4KA depletion by siRNA expression in Huh-7.5 cells, transcomplementation, complementation with the siRNA-resistant PI4KA enhances infectious HCV production 110fold compared to the vector alone, overview. In cell lines expressing the HCV polyprotein, PI4KA siRNAs alter the localization of the HCV NS5A protein, while membranous webs are difficult to detect in HCV-infected cells pretreated with PI4KA siRNAs

723072

additional information

Homo sapiens

-

PI4KIIIalpha isozyme knockout in K-562 cells by siRNA

721713

additional information

Saccharomyces cerevisiae

-

deletion mutant Pik1-(DELTA16-833) has no enzymatic activity

674864

additional information

Saccharomyces cerevisiae

-

depletion of isozymes PI4KIIIalpha and PI4KIIIbeta using siRNA, depletion of PI4KIIIalpha prevents the recruitment of GBF1 to Golgi membranes,phenotypes, overview

722857