2.7.4.25: (d)CMP kinase

This is an abbreviated version!
For detailed information about (d)CMP kinase, go to the full flat file.

Reaction

Synonyms

ATP:CMP phosphotransferase, bacterial cytidylate kinase, CMK, CMP kinase, CMPK, cytidine 5�-monophosphate kinase, cytidine monophosphate kinase, cytidylate kinase, dCMP kinase, deoxycytidine monophosphate kinase, deoxycytidine monophosphokinase, deoxycytidylate kinase, More, P25 protein, prokaryotic cytidylate kinase, SP1603, UCK, UMP-CMP kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.25 (d)CMP kinase

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Results	in table
41245	AA Sequence
1	Application
12	Cloned(Commentary)
10	Cofactor
7	Crystallization (Commentary)
5	General Information
1	General Stability
1	Inhibitors
38	kcat/KM [mM/s]
98	KM Value [mM]
5	Metals/Ions
7	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
210	Organism
4	Pathway
9	PDB ID
25	Protein Variants
8	Purification (Commentary)
1	Reaction
3	Reaction Type
19	Reference
4	Specific Activity [micromol/min/mg]
2	Storage Stability
76	Substrates and Products (Substrate)
5	Subunits
44	Synonyms
1	Systematic Name
15	Temperature Stability [°C]
45	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0083 - 0.65

2',3'-dideoxy-CMP

4 entries

0.085 - 56

ara-CMP

4 entries

0.069 - 103

CMP

14 entries

0.04 - 109

dCMP

14 entries

0.013 - 12.2

UMP

9 entries

0.0083

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.047

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.12

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

0.65

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.085

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.47

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

1.36

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

56

ara-CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.069

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.08

CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.12

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.23

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.26

CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

1.38

CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

1.4

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

4.1

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

22.4

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

52

CMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

63

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

103

CMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

103

CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

103

CMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.04

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.05

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.06

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.071

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.15

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.19

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.45

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

0.73

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

14.5

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

21.1

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

30

dCMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

108

dCMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

108

dCMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

109

dCMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.013

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.054

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.45

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.57

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.82

UMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

0.82

UMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

8.3

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

9.9

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

12.2

UMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276