1.14.13.64: 4-hydroxybenzoate 1-hydroxylase
This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 1-hydroxylase, go to the full flat file.
Word Map on EC 1.14.13.64
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1.14.13.64
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parapsilosis
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hydroquinone
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phenol
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flavoprotein
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fad-dependent
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glucosyltransferase
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shikimate
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arbutin
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3-hydroxybenzoate
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6-hydroxylase
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1,4-dihydroxybenzene
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synthesis
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gentisate
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pharmacology
- 1.14.13.64
- parapsilosis
- hydroquinone
- phenol
- flavoprotein
-
fad-dependent
-
glucosyltransferase
- shikimate
- arbutin
- 3-hydroxybenzoate
-
6-hydroxylase
- 1,4-dihydroxybenzene
- synthesis
- gentisate
- pharmacology
Reaction
Synonyms
4-hydroxybenzoate 1-hydroxylase, 4-hydroxybenzoate 1-hydroxylase (decarboxylating), 4-hydroxybenzoate 1-monooxygenase, 4HB1H, Cp_4HB1H, FAD-dependent 4-hydroxybenzoate hydroxylase, MNX1, Mnx1p, Oxygenase, 4-hydroxybenzoate 1-mono, PHBH, V1H, vanillate 1-hydroxylase
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Engineering
Engineering on EC 1.14.13.64 - 4-hydroxybenzoate 1-hydroxylase
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additional information
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Yarrowia lipolytica is engineered for enhanced production of arbutin. To construct the arbutin synthetic pathway in Yarrowia lipolytica, three genes, chorismate pyruvate-lyase (UbiC), 4-hydroxybenzoate 1-hydroxylase (MNX1), and hydroquinone glucosyltransferase (AS), are codon-optimized and heterologously expressed in Yarrowia lipolytica strain polf (leucine and uracil auxotroph). To maximize arbutin production, seven arbutin-biosynthesis molecular targets are overexpressed, and individual strengthening of DHS1 and DHS2 leads to an 8.9 and 7.8fold improvement in arbutin yield, respectively. Through optimization, a maximum arbutin titer of 8.6 g/l is achieved using the finally engineered Yarrowia lipolytica strain po1f-At0 Improvement in arbutin yields by glucose feeding