1.2.1.89: D-glyceraldehyde dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about D-glyceraldehyde dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

AldA, ALDH, GADH, GLD, glyceraldehyde dehydrogenase, Pto0332, Ta0809, TaAlDH

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.89 D-glyceraldehyde dehydrogenase (NADP+)

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Results	in table
5	Activating Compound
11	AA Sequence
2	Application
6	Cloned(Commentary)
9	Cofactor
2	Crystallization (Commentary)
6	General Information
9	Inhibitors
17	kcat/KM [mM/s]
23	KM Value [mM]
10	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
19	Organism
5	Pathway
16	PDB ID
6	pH Optimum
3	pH Range
1	pH Stability
21	Protein Variants
6	Purification (Commentary)
1	Reaction
13	Reference
5	Specific Activity [micromol/min/mg]
41	Substrates and Products (Substrate)
15	Subunits
24	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Temperature Stability [°C]
17	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.2.1.89 - D-glyceraldehyde dehydrogenase (NADP+)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

structures of wild-type and mutant F34M/S405N, to 1.95 A resolution for wild-type and 2.14 and 2.10 A resolution for the mutant

Thermoplasma acidophilum

Q9HK01

739818

wild type enzyme, sitting drop vapor diffusion method, using 50 mM MOPS, 50 mM sodium HEPES pH 7.5, 20% (w/v) ethylene glycol, 10% (w/v) PEG 8000, 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic). Mutant enzyme F34M/S405N, sitting drop vapor diffusion method, using 50 mM MES, 50 mM imidazole pH 6.5, 8.3% (w/v) PEG 8000, 16.7% (w/v) ethylene glycol, 20 mM sodium formate, 20 mM sodium citrate, 20 mM sodium oxamate, 20 mM ammonium acetate, 20 mM sodium potassium tartrate

Thermoplasma acidophilum

Q9HK01

762564