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1.3.1.119: chlorobenzene dihydrodiol dehydrogenase

This is an abbreviated version!
For detailed information about chlorobenzene dihydrodiol dehydrogenase, go to the full flat file.

Word Map on EC 1.3.1.119

Reaction

(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol
+
NAD+
=
3-chlorocatechol
+
NADH
+
H+

Synonyms

3,6-dichlorobenzene DDH, cbzB, CDD, chlorobenzene cis-dihydrodiol dehydrogenase, cis-chlorobenzene dihydrodiol dehydrogenase, TcbB, tecB

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.119 chlorobenzene dihydrodiol dehydrogenase

Cloned

Cloned on EC 1.3.1.119 - chlorobenzene dihydrodiol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all open reading frames from the tcbAB cluster are cloned in the expression vector pET8c under transcriptional control of the T7 promoter in Escherichia coli BL21(DE3)
expression in Escherichia coli DH5a(pTCB149)
expression in Escherichia coli DH5alpha
-
the tecB gene is cloned together with the tecA gene into Escherichia coli DH5alpha