1.3.1.81: (+)-pulegone reductase

This is an abbreviated version!
For detailed information about (+)-pulegone reductase, go to the full flat file.

Word Map on EC 1.3.1.81

1.3.1.81

peppermint

+-pulegone

mentha

monoterpene

piperita

+-menthofuran

monoterpenoids

glandular

trichomes

geranyl

peltate

terpenoids

p-menthane

Reaction

Synonyms

(+)-pulegone reductase, More, pulegone reductase

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.1.81 (+)-pulegone reductase

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Results	in table
2	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
11	Cofactor
3	Expression
5	General Information
1	General Stability
1	IC50 Value
3	Inhibitors
1	Ki Value [mM]
4	KM Value [mM]
2	Localization
4	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
11	Organism
3	Pathway
1	PDB ID
2	pH Optimum
1	pH Range
1	pI Value
3	Purification (Commentary)
2	Reaction
11	Reference
14	Source Tissue
1	Specific Activity [micromol/min/mg]
19	Substrates and Products (Substrate)
2	Subunits
10	Synonyms
1	Systematic Name
2	Turnover Number [1/s]

Purification

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Purification on EC 1.3.1.81 - (+)-pulegone reductase

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native enzyme partially by isolation of secretory cells from leaves

Mentha x piperita

689800

recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision

Mentha x piperita

660251

recombinant enzyme from Escherichia coli strain BL21(DE3), native enzyme by oil gland secretory cell isolation procedure, two steps of anion exchange chromatography, hydroxyapatite chromatography, and beta-NADPH affinity chromatography, the activity is rapidly lost during purification on each matrix, 10fold purification accompanied by loss of over 98% of starting activity

Mentha x piperita

684664