1.3.98.1: dihydroorotate dehydrogenase (fumarate)
This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (fumarate), go to the full flat file.
Word Map on EC 1.3.98.1
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1.3.98.1
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pyrimidine
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leflunomide
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brequinar
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uridine
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plasmodium
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falciparum
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ubiquinone
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teriflunomide
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dhods
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1.3.3.1
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ump
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dihydro-orotate
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triazolopyrimidine
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atovaquone
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1.3.99.11
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dysostosis
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analysis
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medicine
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acrofacial
- 1.3.98.1
- pyrimidine
- leflunomide
- brequinar
- uridine
- plasmodium
- falciparum
- ubiquinone
- teriflunomide
-
dhods
-
1.3.3.1
- ump
-
dihydro-orotate
- triazolopyrimidine
- atovaquone
-
1.3.99.11
- dysostosis
- analysis
- medicine
-
acrofacial
Reaction
Synonyms
(DHO) dehydrogenase, 4,5-L-dihydroorotate:oxygen oxidoreductase, ACT/DHOD, class 1A DHOD, class 1A DHODH, class 1A dihydroorotate dehydrogenase, DHOD, DHOD1, DHOD2, DHOD3, DHODA, DHOdehase, DHODH, DHODH-1A, dihydroorotate dehydrogenase, dihydroorotate dehydrogenase class 1A, Dihydroorotate oxidase, EC 1.3.3.1, LmDHODH, More, oxidase, dihydroorotate, TcDHOD
ECTree
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Cloned
Cloned on EC 1.3.98.1 - dihydroorotate dehydrogenase (fumarate)
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expression in Escherichia coli
genes subcloned int the EcoRI site of pUC18, PCR products cloned in vector pET100/D-TOPO, recombinant DHOD1 and DHOD2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RP
into vector pET-28a and overexpressed in Escherichia coli strain BL21(DE3) as a histidine-tag fusion protein
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into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL
ligated into the pZErO-2 vector and transformation of the Escherichia coli strain TOP 10, inserted into the pET3a expression vector and transformation of Escherichia coli BL21
transformation in Escherichia coli for detection of pyrimidine prototrophic colonies with a resistance of ampicillin
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