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Information on EC 1.1.1.116 - D-arabinose 1-dehydrogenase (NAD+)
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EC Tree
1.1.1.116 D-arabinose 1-dehydrogenase (NAD+)Specify your search results
Word Map
- 1.1.1.116
- l-galactose
- nadp+
- l-fucose
-
d-erythroascorbic
- d-arabinono-1,4-lactone
-
nadp+-dependent
-
five-carbon
- l-ascorbate
- dehydratase
-
sulfolobus
- acetoin
- 2-oxoglutarate
- archaea
- solfataricus
-
sephacryl
- l-xylose
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
d-arabinose dehydrogenase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ARA
ARA1
Ara2p
arabinose(fucose)dehydrogenase
-
-
-
-
D-arabinose dehydrogenase
dehydrogenase, D-arabinose
-
-
-
-
NAD(+)-specific D-arabinose dehydrogenase
NAD-pentose-dehydrogenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-arabinose + NAD+ = D-arabinono-1,4-lactone + NADH + H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
D-arabinose:NAD+ 1-oxidoreductase
-
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CAS REGISTRY NUMBER
COMMENTARY
37250-47-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-ribose + NAD+
2-deoxy-D-ribono-1,4-lactone + NADH
-
no substrate for fungal enzyme
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
the enzyme is involved in the biosynthesis of D-erythroascorbic acid and D-erythroascorbate glucoside from exogeneous D-arabinose in the fungus, overview, D-erythroascorbate is more stable to autoxidation and accumulates in the organism
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
preferred sugar substrate
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
preferred sugar substrate
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
by far the best substrate
-
?, ir
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
irreversible reaction at pH 10
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?, ir
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
one of a variety of NAD+ or NADP+ requiring arabinose dehydrogenases, see also 1.1.1.115
-
-
?
D-xylose + NAD+
D-xylono-1,4-lactone + NADH
-
no substrate for bacterial enzyme
-
-
?
additional information
?
-
-
no substrates for the fungal enzyme are D-fucose, L-xylose, 6-phosphogluconate
-
-
?
additional information
?
-
-
no substrates for the bacterial enzyme are D-lyxose, D-fucose, L-rhamnose, 2-deoxy-D-glucose, D-fructose, sucrose, xylitol, L-arabitol, D-arabitol, ribitol, D-sorbitol, D-mannitol, D-galactose, D-mannose
-
-
?
additional information
?
-
ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose
-
-
?
additional information
?
-
-
ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose
-
-
?
additional information
?
-
ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
the enzyme is involved in the biosynthesis of D-erythroascorbic acid and D-erythroascorbate glucoside from exogeneous D-arabinose in the fungus, overview, D-erythroascorbate is more stable to autoxidation and accumulates in the organism
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose
-
-
?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
-
one of a variety of NAD+ or NADP+ requiring arabinose dehydrogenases, see also 1.1.1.115
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-arabinose
substrate inhibition of Ara2p at concentrations above 5 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00071
NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 9
20% of maximal activity at pH 6.5 and pH 9.0, pH profile
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ARA2; gene YMR041c or ARA2, isozyme Ara2p
SwissProt
brenda
recombinant enzyme overexpressed in Saccharomyces cerevisiae and Zygosaccharomyces bailii
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
-
ARA1 can only be detected in yeast peptone dextrose 2-DE gel images, thus higher reductive efficiency and higher cofactor availability are obtained with the alternation of cultivation condition (mainly growth medium)
brenda
additional information
-
ARA1 can only be detected in yeast peptone dextrose 2-DE gel images, thus higher reductive efficiency and higher cofactor availability are obtained with the alternation of cultivation condition (mainly growth medium)
-
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ARA2_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
335
0
38220
Swiss-Prot
other Location (Reliability: 3)
K0IVQ5_AMPXN
Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)
302
0
34027
TrEMBL
-
A3LTU8_PICST
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
326
0
36897
TrEMBL
other Location (Reliability: 1)
W1QGZ4_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
332
0
37624
TrEMBL
other Location (Reliability: 3)
F0STM9_RUBBR
Rubinisphaera brasiliensis (strain ATCC 49424 / DSM 5305 / JCM 21570 / IAM 15109 / NBRC 103401 / IFAM 1448)
316
0
35520
TrEMBL
-
A0A8J5EJK0_9ASCO
330
0
36481
TrEMBL
other Location (Reliability: 2)
Q88D95_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
270
0
29123
TrEMBL
-
A0A3G2S387_9BASI
437
0
48370
TrEMBL
other Location (Reliability: 2)
A0A8J5AZ70_9ASCO
330
0
36465
TrEMBL
other Location (Reliability: 1)
A0A0F8BWA0_CERFI
386
0
41200
TrEMBL
other Location (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
41000
42000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity
additional information
-
ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity
additional information
-
ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overview
-
gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE