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Information on EC 1.14.14.107 - ent-kaurenoic acid oxidase

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IUBMB Comments
Requires cytochrome P-450. Catalyses three sucessive oxidations of ent-kaurenoic acid. The second step includes a ring-B contraction giving the gibbane skeleton. In pumpkin (Cucurbita maxima) ent-6alpha,7alpha-dihydroxykaur-16-en-19-oate is also formed.
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
oskao, hvkao1, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 = gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
second step, The second step includes a ring-B contraction giving the gibbane skeleton
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ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
show the reaction diagram
overall reaction
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ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
first step
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gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2 = gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
third step
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