1.4.1.3: glutamate dehydrogenase [NAD(P)+] This is an abbreviated version! For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file .
Reaction
L-glutamate +
H2O +
NAD(P)+ =
2-oxoglutarate +
NH3 +
NAD(P)H +
H+
Synonyms At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH
ECTree
pH Range
pH Range on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]
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6.5 - 8
pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity
7 - 9
pH 7.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity
7.5 - 8
-
half activity of glutamate deamination compared to pH 9.5
7.5 - 8.8
-
NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8
7.75 - 8
-
maximal specific activity in TRA buffer
7.9 - 9
-
activity range, profile overview
8 - 9.2
-
deamination, more than 50% of activity maximum at pH 8.0 and 9.2
7 - 8
25-30% less active at pH 7.0 than at pH 7.5 and 8.0
7 - 8
equally active at pH 7.0 and 8.0
7 - 8
-
lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination