2.1.1.160: caffeine synthase
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For detailed information about caffeine synthase, go to the full flat file.
Reaction
Synonyms
1-N-methyltransferase, 1NMT, 3, 7-dimethylxanthine methyltransferase, 3,7-dimethylxanthine methyltransferase, 7-N-methylxanthine methyltransferase, caffeine synthase, caffeine synthase 1, CCS, CCS1, coffee caffeine synthase 1, CS, CtCS6, DXMT, DXMT1, N-1 methyltransferase, TCS, TCS1, tea caffeine synthase, tea caffeine synthase 1, theobromine 1-N-methyltransferase
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Application
Application on EC 2.1.1.160 - caffeine synthase
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agriculture
expression of the caffeine biosynthesis enzymes in transgenic crop plants may protect against the crop damaging larvae of pests
analysis
biotechnology
large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea
food industry
synthesis
production of caffeine in metabolically engineered Escherichia coli by the expression of caffeine synthase TCS1. Caffeine accumulation is increased using higher-level expression of the target enzymes, and enhancement of xanthine and S-adenosyl-L-methionine biosynthesis. The final strain produces up to 21.46 mg/l caffeine from 20 g/l of glucose in shake flask culture, yielding caffeine up to 2.96 mg/g glucose
method to produce a recombinant caffeine synthase from guarana in Escherichia coli and its purification by affinity chromatography
analysis
protocol for the quantification of caffeine and for measuring the transcript levels of the gene (CCS1) encoding caffeine synthase in cell suspensions of Coffea arabica L. as well as its activity
large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea
food industry
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producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA
food industry
treatment of endosperms with 0.05 mM salicylic acid leads to 11.8% increase in theobromine content. Caffeine shows an increase in both methyl jasmonate (14.4% increase) and salicylic acid (50 microM, 14.8% increase) treatments
food industry
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treatment of endosperms with 0.05 mM salicylic acid leads to 11.8% increase in theobromine content. Caffeine shows an increase in both methyl jasmonate (14.4% increase) and salicylic acid (50 microM, 14.8% increase) treatments
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