2.1.1.160: caffeine synthase

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For detailed information about caffeine synthase, go to the full flat file.

Reaction

Synonyms

1-N-methyltransferase, 1NMT, 3, 7-dimethylxanthine methyltransferase, 3,7-dimethylxanthine methyltransferase, 7-N-methylxanthine methyltransferase, caffeine synthase, caffeine synthase 1, CCS, CCS1, coffee caffeine synthase 1, CS, CtCS6, DXMT, DXMT1, N-1 methyltransferase, TCS, TCS1, tea caffeine synthase, tea caffeine synthase 1, theobromine 1-N-methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.160 caffeine synthase

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Results	in table
1	Activating Compound
18	AA Sequence
9	Application
1	CAS Registry Number
19	Cloned(Commentary)
4	Cofactor
3	Crystallization (Commentary)
11	Expression
6	General Information
7	Inhibitors
5	KM Value [mM]
2	Localization
4	Metals/Ions
12	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
39	Organism
6	Pathway
9	pH Optimum
2	pH Range
2	pI Value
11	Protein Variants
5	Purification (Commentary)
5	Reaction
39	Reference
45	Source Tissue
5	Specific Activity [micromol/min/mg]
71	Substrates and Products (Substrate)
9	Subunits
44	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.1.1.160 - caffeine synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution

Coffea canephora

A4GE70

667101

purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 2 mM S-adenosyl-L-cysteine, and 2 mM theobromine, 1-3 days, 20°C, plate-like crystals, X-ray diffraction structure determination and analysis at 2.0-2.7 A resolution, molecular replacement

Coffea canephora

A4GE70

670598

using Linbro plates and the conventional hanging-drop technique, in the presence of the demethylated cofactor S-adenosyl-L-cysteine or theobromine

Coffea canephora

A4GE70

684218