2.1.1.228: tRNA (guanine37-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine37-N1)-methyltransferase, go to the full flat file.

Reaction

Synonyms

At3g56120, aTrm5a methyltransferase, aTrm5a/Taw22-like enzyme, AtTrm5a, bacterial tRNA (guanine37-N1)-methyltransferase, bacterial tRNA-(N1G37) methyltransferase, EC 2.1.1.31, EcTrmD, HiTrmD, HsTrm5, m1G37 tRNA methyltransferase, m1G37-forming tRNA methyltransferase, Mj-Trm5, MJ0883, MjTrm5, MjTrm5b, mono-functional methyltransferase, More, MtbTrmD, NEQ228, PA14_15990, PAB2272, PAB_RS03940, PaTrm5a, PaTrm5b, PaTrmD, SaTrmD, Ta0997, TAW22, transfer RNA (m1G37) methyltransferase, Trm1, TRM5, Trm5a, Trm5a/Taw22-like enzyme, trm5b, Trm5p, TrmD, TRMT5, tRNA (guanine(37)-N1)-methyltransferase 1, tRNA (guanine37-N1)-methyltransferase, tRNA (guanosine-1) methyltransferase, tRNA (m1G37) methyltransferase, tRNA (m1G37)-methyltransferase, tRNA m1G37 methyltransferase, tRNA methyltransferase, tRNA methyltransferase 5, tRNA methyltransferase D, tRNA(m(1)G37)methyltransferase, tRNA(m1G37) methyltransferase, tRNA(m1G37) MTase, tRNA(m1G37)methyltransferase, tRNA-(N1G37) methyltransferase, tRNAPhe:imG2 methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.228 tRNA (guanine37-N1)-methyltransferase

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Results	in table
1	Activating Compound
43500	AA Sequence
16	Application
31	Cloned(Commentary)
49	Cofactor
17	Crystallization (Commentary)
98	Disease/ Diagnostics
3	Expression
219	General Information
3	General Stability
89	IC50 Value
190	Inhibitors
40	kcat/KM [mM/s]
27	Ki Value [mM]
91	KM Value [mM]
8	Localization
44	Metals/Ions
8	Molecular Weight [Da]
116	Natural Substrates/ Products (Substrates)
168	Organism
3	Pathway
201	PDB ID
29	pH Optimum
5	pH Range
126	Protein Variants
23	Purification (Commentary)
9	Reaction
103	Reference
3	Source Tissue
269	Substrates and Products (Substrate)
58	Subunits
226	Synonyms
1	Systematic Name
28	Temperature Optimum [°C]
3	Temperature Stability [°C]
43	Turnover Number [1/s]

Natural Substrates Products

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of the Enzyme Summary Page.

Natural Substrates Products on EC 2.1.1.228 - tRNA (guanine37-N1)-methyltransferase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

3 entries

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

3 entries

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

2 entries

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

46 entries

S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)

Arabidopsis thaliana

Q93YU6

enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro

757863

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

5 entries

S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)

Escherichia coli

P0A873

-

756774

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

2 entries

additional information

?

-

12 entries

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi Orsay

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Pyrococcus abyssi Orsay

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Aquifex aeolicus

O67463

-

756163, 756631

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Arabidopsis thaliana

Q93YU6

-

757863

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

-

712739, 720240, 721029, 721035, 735927

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

P0A873

-

661154, 736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

-

736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

P0A873

-

756161, 756163, 756631, 756774, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

A0A0D0GZF5

-

756163, 756631, 757790

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae ATCC 51907

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae DSM 11121

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae DSM 11121

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae KW20

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae RD

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

-

-

735421

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

Q32P41

-

661154, 737226

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

-

-

737226

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

Q32P41

-

757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

-

-

720240, 721029, 721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

Q58293

-

712739, 721026, 756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii ATCC 43067

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii DSM 2661

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii JAL-1

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii JCM 10045

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii NBRC 100440

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Mycobacterium tuberculosis

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Mycobacteroides abscessus

-

-

757408

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

Q02RL6

-

755697, 756152

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

756152

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

Q02RL6

-

756153, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

-

755697, 756152, 756153, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pyrococcus abyssi

Q9V2G1

-

756161, 758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pyrococcus abyssi Orsay

Q9V2G1

-

756161, 758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae

P38793

-

756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae

P38793

Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs

687609

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae ATCC 204508

P38793

-

756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Staphylococcus aureus

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Streptococcus pneumoniae

-

-

662010

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Thermotoga maritima

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trypanosoma brucei brucei

Q57X10

TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Methanocaldococcus jannaschii

Q58293

-

758348

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Methanocaldococcus jannaschii DSM 2661

Q58293

-

758348

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

758348, 758376

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

-

-

758376

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

Q9V0Q0

-

755960

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

756161

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

Pyrococcus abyssi Orsay

Q9V2G1

-

756161

-

-

?

additional information

?

-

Aquifex aeolicus

O67463

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Escherichia coli

-

TrmD catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon

720240

-

-

?

additional information

?

-

Escherichia coli

P0A873

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Haemophilus influenzae

A0A0D0GZF5

TrmD is a bacterial enzyme with a trefoil-knot in the active site, involving three crossings of the protein backbone through a loop. TrmD catalyzes methyl transfer from AdoMet to the N1 of G37 on the 3' side of the tRNA anticodon

757790

-

-

-

additional information

?

-

Haemophilus influenzae

A0A0D0GZF5

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Methanocaldococcus jannaschii

-

Trm5 catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon

720240

-

-

?

additional information

?

-

Pyrococcus abyssi

Q9V2G1

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758348

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758376

-

-

-

additional information

?

-

Pyrococcus abyssi

-

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758376

-

-

-

additional information

?

-

Salmonella enterica subsp. enterica serovar Typhimurium

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-