2.1.1.228: tRNA (guanine37-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine37-N1)-methyltransferase, go to the full flat file.

Reaction

Synonyms

At3g56120, aTrm5a methyltransferase, aTrm5a/Taw22-like enzyme, AtTrm5a, bacterial tRNA (guanine37-N1)-methyltransferase, bacterial tRNA-(N1G37) methyltransferase, EC 2.1.1.31, EcTrmD, HiTrmD, HsTrm5, m1G37 tRNA methyltransferase, m1G37-forming tRNA methyltransferase, Mj-Trm5, MJ0883, MjTrm5, MjTrm5b, mono-functional methyltransferase, More, MtbTrmD, NEQ228, PA14_15990, PAB2272, PAB_RS03940, PaTrm5a, PaTrm5b, PaTrmD, SaTrmD, Ta0997, TAW22, transfer RNA (m1G37) methyltransferase, Trm1, TRM5, Trm5a, Trm5a/Taw22-like enzyme, trm5b, Trm5p, TrmD, TRMT5, tRNA (guanine(37)-N1)-methyltransferase 1, tRNA (guanine37-N1)-methyltransferase, tRNA (guanosine-1) methyltransferase, tRNA (m1G37) methyltransferase, tRNA (m1G37)-methyltransferase, tRNA m1G37 methyltransferase, tRNA methyltransferase, tRNA methyltransferase 5, tRNA methyltransferase D, tRNA(m(1)G37)methyltransferase, tRNA(m1G37) methyltransferase, tRNA(m1G37) MTase, tRNA(m1G37)methyltransferase, tRNA-(N1G37) methyltransferase, tRNAPhe:imG2 methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.228 tRNA (guanine37-N1)-methyltransferase

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Results	in table
1	Activating Compound
43500	AA Sequence
16	Application
31	Cloned(Commentary)
49	Cofactor
17	Crystallization (Commentary)
98	Disease/ Diagnostics
3	Expression
219	General Information
3	General Stability
89	IC50 Value
190	Inhibitors
40	kcat/KM [mM/s]
27	Ki Value [mM]
91	KM Value [mM]
8	Localization
44	Metals/Ions
8	Molecular Weight [Da]
116	Natural Substrates/ Products (Substrates)
168	Organism
3	Pathway
201	PDB ID
29	pH Optimum
5	pH Range
126	Protein Variants
23	Purification (Commentary)
9	Reaction
103	Reference
3	Source Tissue
269	Substrates and Products (Substrate)
58	Subunits
226	Synonyms
1	Systematic Name
28	Temperature Optimum [°C]
3	Temperature Stability [°C]
43	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.1.1.228 - tRNA (guanine37-N1)-methyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

6 entries

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

5 entries

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

2 entries

S-adenosyl-L-methionine + guanine36 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu

2 entries

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(ACG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(ACG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAArg(CCG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAArg(CCG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAGln(UUG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAGln(UUG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAHis(GUG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAHis(GUG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNALeu(CAG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Aquifex aeolicus tRNAPro(GGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Aquifex aeolicus tRNAPro(GGG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in elongator tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in elongator tRNA

Thermoplasma acidophilum

-

-

729858

-

-

?

S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu

S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu

2 entries

S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNAPro

Escherichia coli

-

-

712739

-

-

?

S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNACys(GCA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNACys(GCA)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNALeu(CAA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNALeu(CAA)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATrp(CCA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATrp(CCA)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Haloferax volcanii tRNATyr(GUA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Haloferax volcanii tRNATyr(GUA)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro

2 entries

S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in in Escherichia coli tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in in Escherichia coli tRNALeu(CAG)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNA(Cys)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNA(Cys)

Methanocaldococcus jannaschii

Q58293

-

701123

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAArg(UCG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAArg(UCG)

Methanocaldococcus jannaschii

Q58293

possessing the sequence G36G37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNACys

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNACys

Methanocaldococcus jannaschii

Q58293

-

711197

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNACys(GCA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNACys(GCA)

Methanocaldococcus jannaschii

Q58293

possessing the sequence A36G37. The enzyme is inactive with mutant forms of Methanocaldococcus jannaschii tRNACys(GCA) containing A37, C37, or U37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC)

Methanocaldococcus jannaschii

Q58293

possessing the sequence C36G37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNALeu(UCG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNALeu(UCG)

Methanocaldococcus jannaschii

Q58293

possessing the sequence G36G37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro

2 entries

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(GGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(GGG)

Methanocaldococcus jannaschii

Q58293

possessing the sequence G36G37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(UGG)

Methanocaldococcus jannaschii

Q58293

possessing the sequence G36G37

662622

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

59 entries

S-adenosyl-L-methionine + guanine37 in tRNAArg

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArg

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAArgCCG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArgCCG

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)

Arabidopsis thaliana

Q93YU6

enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro

757863

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACys

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys

3 entries

S-adenosyl-L-methionine + guanine37 in tRNACysGCA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACysGCA

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAGlnCUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnCUG

3 entries

S-adenosyl-L-methionine + guanine37 in tRNAGlnG36A

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnG36A

Haemophilus influenzae

-

tRNA substrate from Thermotoga maritima

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAGlnG36C

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnG36C

Haemophilus influenzae

-

tRNA substrate from Thermotoga maritima

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAGlnG36U

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnG36U

Haemophilus influenzae

-

tRNA substrate from Thermotoga maritima

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAHis(GUG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHis(GUG)

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAHisGUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHisGUG

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

4 entries

S-adenosyl-L-methionine + guanine37 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu

4 entries

S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)

3 entries

S-adenosyl-L-methionine + guanine37 in tRNALeu(GAC)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(GAC)

Streptococcus pneumoniae

-

-

662010

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu(GAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(GAG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu(UAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(UAG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeuCAG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeuCAG

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

5 entries

S-adenosyl-L-methionine + guanine37 in tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro

2 entries

S-adenosyl-L-methionine + guanine37 in tRNAPro(CGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(CGG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro(GGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(GGG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)

3 entries

S-adenosyl-L-methionine + guanine37 in tRNAProAGG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAProAGG

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNA(Asp) possessing a G36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing a G36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a C36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a C36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence

2 entries

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing an A36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an A36G37 sequence

Escherichia coli

P0A873

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an U36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an U36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe possessing an A36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe possessing an A36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe(GAA)

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe(GAA)

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + inosine37 in yeast tRNAAsp possessing a G36I37 sequence

S-adenosyl-L-homocysteine + N1-methylinosine37 in yeast tRNAAsp possessing a G36I37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

2 entries

additional information

?

-

74 entries

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Nanoarchaeum equitans

Q74NE4

i.e. im-G14, activity of EC 2.1.1.282

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi

Q9V2G1

i.e. im-G14, activity of EC 2.1.1.282

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi Orsay

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 4-demethylwyosine

S-adenosyl-L-homocysteine + isowyosine

Pyrococcus abyssi Orsay

Q9V2G1

i.e. im-G14, activity of EC 2.1.1.282

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Nanoarchaeum equitans

Q74NE4

i.e. yW-86, activity of EC 2.1.1.228

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Pyrococcus abyssi

Q9V2G1

i.e. yW-86, activity of EC 2.1.1.228

758324

-

-

?

S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine

S-adenosyl-L-homocysteine + wyosine

Pyrococcus abyssi Orsay

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

758324

-

-

?

S-adenosyl-L-methionine + guanine36 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu

Escherichia coli

-

G36-substituted tRNA substrate Escherichia coli tRNALeu, TrmD shows a 90fold reduced catalytic efficiency, discrimination between the two sequences of G36 and G37

721035

-

-

?

S-adenosyl-L-methionine + guanine36 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu

Methanocaldococcus jannaschii

-

G36-substituted tRNA substrate Escherichia coli tRNALeu, Trm5 shows a lack of discrimination between the two sequences of G36 and G37

721035

-

-

?

S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu

S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu

Escherichia coli

P0A873

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu

S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro

Escherichia coli

P0A873

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro

Escherichia coli

-

-

712739

-

-

?

S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro

Methanocaldococcus jannaschii

Q58293

-

712739

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Aquifex aeolicus

-

-

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Aquifex aeolicus

O67463

-

756163, 756631

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Aquifex aeolicus

-

the enzyme methylates tRNA transcripts possessing an A36G37 sequence as well as tRNA transcripts possessing a G36G37 sequence. tRNA transcripts possessing pyrimidine36G37 are not methylated at all. The modified nucleoside and the position in yeast tRNA(Phe) transcript are confirmed by LC/MS. Nine truncated tRNA molecules are tested to clarify the additional recognition site. The TrmD protein efficiently methylates the micro helix corresponding to the anti-codon arm. Because the disruption of the anti-codon stem causes the complete loss of the methyl group acceptance activity, the anti-codon stem is essential for the recognition. The existence of the D-arm structure inhibits the activity

673850

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Arabidopsis thaliana

Q93YU6

-

757863

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

-

712739, 720240, 721029, 721035, 735927

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

P0A873

-

661154, 736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

-

736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

P0A873

-

756161, 756163, 756631, 756774, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

P0A873

methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

substrate binding stoichiometry to TrmD, dissociation constants, overview

720240

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

TrmD recognizes N1 and O6 of G37 and the exocyclic 2-amino group of G37 is important for TrmD, also TrmD requires G36 for synthesis of m1G37

721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Escherichia coli

-

the pH-activity profile indicates one proton transfer during the TrmD reaction

735927

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

-

-

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

A0A0D0GZF5

-

756163, 756631, 757790

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae ATCC 51907

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae DSM 11121

-

-

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae DSM 11121

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae DSM 11121

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae KW20

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Haemophilus influenzae RD

P43912

-

756161, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

-

-

735421

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

Q32P41

-

661154, 737226

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

-

-

737226

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

Q32P41

-

757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Homo sapiens

Q32P41

methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

-

-

720240, 721029, 721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

Q58293

-

712739, 721026, 756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

-

-

720240

tight binding of Trm5 to products

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii

-

Trm5 recognizes N1 and O6 of G37, but the exocyclic 2-amino group of G37 is dispensable for Trm5. Trm5 does not require G36

721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii ATCC 43067

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii DSM 2661

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii JAL-1

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii JCM 10045

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Methanocaldococcus jannaschii NBRC 100440

Q58293

-

756161, 757201, 757582

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Mycobacterium tuberculosis

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Mycobacteroides abscessus

-

-

757408

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Nanoarchaeum equitans

Q74NE4

-

758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

Q02RL6

-

755697, 756152

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

756152

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

Q02RL6

-

756153, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa

-

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

-

755697, 756152, 756153, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pyrococcus abyssi

Q9V2G1

-

756161, 758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Pyrococcus abyssi Orsay

Q9V2G1

-

756161, 758324

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae

P38793

-

756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae

P38793

Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs

687609

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae

P38793

methyltransferase activity with tRNA isolated from a DELTAtrm5 mutant strain, as well as with a synthetic mitochondrial initiator tRNA (tRNAMetf). N1-Methylguanine is determined by high pressure liquid chromatography analysis

687609

the site of methylation is guanosine 37 in both mitochondrial tRNAMetf and tRNAPhe, determined by primer extension

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Saccharomyces cerevisiae ATCC 204508

P38793

-

756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412

P36245

-

756631, 756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Staphylococcus aureus

-

-

757409

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Streptococcus pneumoniae

-

-

662010

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Streptococcus pneumoniae

-

the streptococcal enzyme utilizes a sequential mechanism. Nonsubstrate tRNA species, like tRNAThr(GGT), tRNAPhe, and tRNAAla(TGC), bind the enzyme with similar affinities, suggesting that tRNA specificity is achieved via a postbinding events. The streptococcal TrmD requires the complete tRNA structure since it cannot modify the tRNALeu(CAG) minihelix lacking the D, T, and extra loops of complete tRNA. In addition, and consistent with a requirement for G at positions 36 and 37 in the tRNA, the enzyme methylates yeast tRNAAsp possessing a G36G37 sequence with kinetic values that are indistinguishable from those obtained with substrate tRNALeu(CAG) but does not methylate either tRNAAsp possessing a C36G37 sequence or tRNA(Asp) possessing a C36A37 sequence

662010

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Thermotoga maritima

-

-

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA

Thermotoga maritima

-

TrmD catalyzes the N1-methylguanosine (m1G) modification at position 37 in tRNAs with the 36GG37 sequence, using S-adenosyl-L-methionine as the methyl donor

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAArg

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArg

Haemophilus influenzae

-

tRNA substrate from Haemophilus influenzae

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAArg

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArg

Haemophilus influenzae DSM 11121

-

tRNA substrate from Haemophilus influenzae

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAArgCCG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArgCCG

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAArgCCG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArgCCG

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACys

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys

Homo sapiens

Q32P41

-

737226

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACys

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys

Methanocaldococcus jannaschii

-

Methanococcus jannaschii tRNACys

721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACys

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys

Methanocaldococcus jannaschii

Q58293

Methanococcus jannaschii tRNACys

721026

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACysGCA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACysGCA

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNACysGCA

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACysGCA

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAGlnCUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnCUG

Haemophilus influenzae

-

the wild-type Thermotoga maritima tRNAGlnCUG transcript is methylated by Haemophilus influenzae TrmD 2.2 to 99fold more efficiently than the Haemophilus influenzae tRNA transcripts

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAGlnCUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnCUG

Haemophilus influenzae DSM 11121

-

the wild-type Thermotoga maritima tRNAGlnCUG transcript is methylated by Haemophilus influenzae TrmD 2.2 to 99fold more efficiently than the Haemophilus influenzae tRNA transcripts

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAGlnCUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAGlnCUG

Thermotoga maritima

-

-

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAHis(GUG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHis(GUG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAHis(GUG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHis(GUG)

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAHisGUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHisGUG

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAHisGUG

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHisGUG

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trametes pubescens 927 / 4 GUTat10.1 / TREU927

Q57X10

TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trypanosoma brucei brucei

Q57X10

-

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAIleUaU

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU

Trypanosoma brucei brucei

Q57X10

TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37

737228

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu

Escherichia coli

P0A873

-

736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu

Escherichia coli

-

Escherichia coli tRNALeu

721035

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu

Haemophilus influenzae

-

tRNA substrate from Haemophilus influenzae

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu

Haemophilus influenzae DSM 11121

-

tRNA substrate from Haemophilus influenzae

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)

Pseudomonas aeruginosa

Q02RL6

-

755697, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)

Pseudomonas aeruginosa

-

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

-

755697, 758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Methanocaldococcus jannaschii

Q58293

-

758348

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Methanocaldococcus jannaschii DSM 2661

Q58293

-

758348

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

758348, 758376

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

-

-

758376

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPhe

Pyrococcus abyssi

Q9V0Q0

-

755960

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro

Escherichia coli

P0A873

the A37 mutant of EctRNAPro is no substrate for the enzyme

736428

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro

Haemophilus influenzae

-

tRNA substrate from Haemophilus influenzae

737174

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)

Escherichia coli

P0A873

-

756774

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)

Pseudomonas aeruginosa

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)

S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

-

758329

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence

Escherichia coli

P0A873

-

661154

-

-

?

S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence

S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence

Homo sapiens

Q32P41

-

661154

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

Pyrococcus abyssi

Q9V2G1

-

756161

-

-

?

S-adenosyl-L-methionine + wyosine37 in tRNAPhe

S-adenosyl-L-homocysteine + N1-methylwyosine37 in tRNAPhe

Pyrococcus abyssi Orsay

Q9V2G1

-

756161

-

-

?

additional information

?

-

Aquifex aeolicus

-

no activity with guanine37 in yeast tRNAAsp(GUC) possessing a C36G37 sequence, guanine37 in Haloferax volcanii tRNAGlu(UUC) possessing a C36G37 sequence, guanine37 in yeast tRNAPhe A36U mutant(GAU) possessing a U36G37 sequence, guanine37 in Escherichia coli tRNASer(UGA) possessing a A36G37 sequence

673850

-

-

?

additional information

?

-

Aquifex aeolicus

O67463

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Aquifex aeolicus

O67463

TrmD can methylate a truncated tRNA, in which T- and D-arms have been deleted, the anticodon-arm region is mainly protected. The tRNA recognition mechanism of Aquifex aeolicus TrmD shows that a micro-helix RNA corresponding to the anticodon-arm is the minimal substrate for this enzyme

756163

-

-

-

additional information

?

-

Escherichia coli

P0A873

TrmD recognizes the G36pG37 motif preferentially and does not methylate inosine. The TrmD enzyme is tolerant of alterations in tRNA-protein tertiary interactions as long as the core tRNA structure and the G36pG37 are present

661154

-

-

?

additional information

?

-

Escherichia coli

-

TrmD recognizes the G36pG37 motif preferentially and does not methylate inosine. The TrmD enzyme is tolerant of alterations in tRNA-protein tertiary interactions as long as the core tRNA structure and the G36pG37 are present

661154

-

-

?

additional information

?

-

Escherichia coli

-

TrmD catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon

720240

-

-

?

additional information

?

-

Escherichia coli

-

recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, TrmD shows a more rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in EctRNALeu, to design the optimal substrate for TrmD. All but deoxyG of these analogs probed the Watson-Crick basepairing interface of G37

721035

-

-

?

additional information

?

-

Escherichia coli

P0A873

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Escherichia coli

P0A873

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Escherichia coli

P0A873

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Escherichia coli

P0A873

TrmD can methylate a truncated tRNA, in which T- and D-arms have been deleted, the anticodon-arm region is mainly protected

756163

-

-

-

additional information

?

-

Haemophilus influenzae

A0A0D0GZF5

TrmD is a bacterial enzyme with a trefoil-knot in the active site, involving three crossings of the protein backbone through a loop. TrmD catalyzes methyl transfer from AdoMet to the N1 of G37 on the 3' side of the tRNA anticodon

757790

-

-

-

additional information

?

-

Haemophilus influenzae

A0A0D0GZF5

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

-

-

-

additional information

?

-

Haemophilus influenzae

P43912

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Haemophilus influenzae

P43912

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Haemophilus influenzae

A0A0D0GZF5

TrmD can methylate a truncated tRNA, in which T- and D-arms have been deleted, the anticodon-arm region is mainly protected

756163

-

-

-

additional information

?

-

Haemophilus influenzae ATCC 51907

P43912

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Haemophilus influenzae ATCC 51907

P43912

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Haemophilus influenzae DSM 11121

P43912

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Haemophilus influenzae DSM 11121

P43912

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Haemophilus influenzae KW20

P43912

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Haemophilus influenzae KW20

P43912

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Haemophilus influenzae RD

P43912

proposed model for the TrmD enzymatic cycle which consists of the AdoMet-binding, tRNA-binding, and methyl transfer stages, overview. Anticodon-branch recognition and detection of position 37, interaction analysis of TrmD with G36 and G37

756161

-

-

-

additional information

?

-

Haemophilus influenzae RD

P43912

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the TrmD reaction is the chemistry of methyl transfer

757582

-

-

-

additional information

?

-

Homo sapiens

Q32P41

guanosine37-methylation by TRM5 occurs regardless of the nature of the nucleotide at position 36. TRM5 also methylates inosine at position 37. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity. The enzyme does not methylate adenosine37, cytosine37 or uridine37 in tRNA. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity

661154

-

-

?

additional information

?

-

Homo sapiens

-

guanosine37-methylation by TRM5 occurs regardless of the nature of the nucleotide at position 36. TRM5 also methylates inosine at position 37. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity. The enzyme does not methylate adenosine37, cytosine37 or uridine37 in tRNA. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity

661154

-

-

?

additional information

?

-

Homo sapiens

Q32P41

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii

Q58293

the enzyme is specific for methylation of guanine37 in tRNA. No methylation of tRNAArg(UCU) possessing the sequence U36G37

662622

-

-

?

additional information

?

-

Methanocaldococcus jannaschii

-

the enzyme is specific for methylation of guanine37 in tRNA. No methylation of tRNAArg(UCU) possessing the sequence U36G37

662622

-

-

?

additional information

?

-

Methanocaldococcus jannaschii

-

Trm5 catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon

720240

-

-

?

additional information

?

-

Methanocaldococcus jannaschii

-

recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, Trm5 shows a less rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in MjtRNACys, to design the optimal substrate for Trm5

721035

-

-

?

additional information

?

-

Methanocaldococcus jannaschii

Q58293

structure of Trm5 active site bound to tRNA and S-adenosyl-L-methionine, induced fit for active-site assembly, detailed overview. E185 is crucial both for general base catalysis and for the conformational change that precedes catalysis

721026

-

-

?

additional information

?

-

Methanocaldococcus jannaschii

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii

Q58293

structural basis for substrate recognition, the D1 domain of the enzyme undergoes large conformational changes upon the binding of tRNA, the enzyme recognizes the overall shape of tRNA, overview. Enzyme-substrate interactions in the catalytic domain, D1 domain ofMjTrm5b transitions, overview

758348

-

-

-

additional information

?

-

Methanocaldococcus jannaschii

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii ATCC 43067

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii ATCC 43067

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii ATCC 43067

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii DSM 2661

Q58293

structural basis for substrate recognition, the D1 domain of the enzyme undergoes large conformational changes upon the binding of tRNA, the enzyme recognizes the overall shape of tRNA, overview. Enzyme-substrate interactions in the catalytic domain, D1 domain ofMjTrm5b transitions, overview

758348

-

-

-

additional information

?

-

Methanocaldococcus jannaschii DSM 2661

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii DSM 2661

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii DSM 2661

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JAL-1

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JAL-1

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JAL-1

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JCM 10045

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JCM 10045

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii JCM 10045

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Methanocaldococcus jannaschii NBRC 100440

Q58293

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

?

-

Methanocaldococcus jannaschii NBRC 100440

Q58293

the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet

757201

-

-

-

additional information

?

-

Methanocaldococcus jannaschii NBRC 100440

Q58293

radioactive assay method development and evaluation using labeled S-adenosyl-L-methionine and unlabeled tRNA, detailed overview. The slow step of the Trm5 reaction is after methyl transfer and is associated with release of the m1G37-tRNA product

757582

-

-

-

additional information

?

-

Nanoarchaeum equitans

Q74NE4

Nanoarchaeum equitans NEQ228 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading tothe formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant

758324

-

-

-

additional information

?

-

Pseudomonas aeruginosa

Q02RL6

substrate specificity, mass spectrometric analysis confirms the G36G37-containing tRNAs Leu(GAG), Leu(CAG), Leu(UAG), Pro(GGG), Pro(UGG), Pro(CGG), and His(GUG) as PaTrmD substrates, overview. PaTrmD catalyzes m1G formation in synthetic tRNA substrates. PaTrmD catalyzes m1G at position 37 in the tRNA anticodon loop. Preparation of tRNA substrates by in vitro transcription, product determination by mass spectrometry

758329

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

substrate specificity, mass spectrometric analysis confirms the G36G37-containing tRNAs Leu(GAG), Leu(CAG), Leu(UAG), Pro(GGG), Pro(UGG), Pro(CGG), and His(GUG) as PaTrmD substrates, overview. PaTrmD catalyzes m1G formation in synthetic tRNA substrates. PaTrmD catalyzes m1G at position 37 in the tRNA anticodon loop. Preparation of tRNA substrates by in vitro transcription, product determination by mass spectrometry

758329

-

-

-

additional information

?

-

Pseudomonas aeruginosa

Q02RL6

TrmD luminescence assay development

755697

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

TrmD luminescence assay development

755697

-

-

-

additional information

?

-

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

TrmD luminescence assay development

755697

-

-

-

additional information

?

-

Pseudomonas aeruginosa UCBPP-PA14

Q02RL6

substrate specificity, mass spectrometric analysis confirms the G36G37-containing tRNAs Leu(GAG), Leu(CAG), Leu(UAG), Pro(GGG), Pro(UGG), Pro(CGG), and His(GUG) as PaTrmD substrates, overview. PaTrmD catalyzes m1G formation in synthetic tRNA substrates. PaTrmD catalyzes m1G at position 37 in the tRNA anticodon loop. Preparation of tRNA substrates by in vitro transcription, product determination by mass spectrometry

758329

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758348

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758376

-

-

-

additional information

?

-

Pyrococcus abyssi

-

bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)

758376

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V0Q0

no activity of Trm5b with 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe (cf. EC 2.1.1.282)

755960

-

-

-

additional information

?

-

Pyrococcus abyssi

-

no activity of Trm5b with 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe (cf. EC 2.1.1.282)

755960

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

Pyrococcus abyssi PAB2272 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant

758324

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

structural basis for substrate recognition, the D1 domain of the enzyme undergoes large conformational changes upon the binding of tRNA. The enzyme recognizes the overall shape of tRNA. PaTrm5a adopts distinct open conformations before and after the binding of tRNA. Enzyme-substrate interactions in the catalytic domain. The anticodon interactions mostly concentrate on the A36-G37-A38 triplet. Proposed reaction mechanism of Trm5a with modified yeast tRNAPhe, overview

758348

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

substrate-binding modes of PaTrm5a, and recognition of substrate analogues, overview

758376

-

-

-

additional information

?

-

Pyrococcus abyssi

-

substrate-binding modes of PaTrm5a, and recognition of substrate analogues, overview

758376

-

-

-

additional information

?

-

Pyrococcus abyssi

Q9V2G1

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

-

-

-

additional information

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Pyrococcus abyssi Orsay

Q9V2G1

tRNA recognition by Trm5, detailed overview. The structure of positions 33-37 in the anticodon loop is largely altered from the canonical tRNA structure, and the target G37 is flipped out into the catalytic pocket formed by the D2 and D3 domains. The flipped G37 is recognized in a guanosine-specific manner by the side chains of Arg145 and Asn265, and the N1-atom (the methylation atom) of G37 is located next to the methyl moiety of AdoMet. The adequate interaction between D1 and tRNA enables the catalytic D2-D3 to perform the m1G37 methylation. The m1G37 methylation is achieved by a sensor-effector mechanism in which the affinity of Trm5 for tRNA increases only when the sensor (D1) confirms the completion of the L-shape formation and the catalytically competent effector (D2-D3) is recruited to the tRNA

756161

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additional information

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Pyrococcus abyssi Orsay

Q9V2G1

Pyrococcus abyssi PAB2272 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant

758324

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additional information

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Salmonella enterica subsp. enterica serovar Typhimurium

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

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additional information

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Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

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additional information

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Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412

P36245

TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon

756631

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