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2.3.1.16: acetyl-CoA C-acyltransferase

This is an abbreviated version!
For detailed information about acetyl-CoA C-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.16

Reaction

acyl-CoA
+
acetyl-CoA
=
CoA
 +
3-oxoacyl-CoA

Synonyms

2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/3-oxoacyl-CoA thiolase, 3-KAT, 3-ketoacyl CoA thiolase, 3-ketoacyl coenzyme A thiolase, 3-ketoacyl coenzyme-A thiolase, 3-ketoacyl thiolase, 3-ketoacyl-CoA thiolase, 3-ketothiolase, 3-oxoacyl-CoA thiolase, 3-oxoacyl-coenzyme A thiolase, 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, 6-oxoacyl-CoA thiolase, ACAA2, acetoacetyl-CoA beta-ketothiolase, acetyl-CoA acyltransferase, acyl-CoA-thiolase, acyltransferase, acetyl coenzyme A, beta-ketoacyl coenzyme A thiolase, beta-ketoacyl-CoA thiolase, beta-ketoadipyl coenzyme A thiolase, beta-ketoadipyl-CoA thiolase, beta-ketothiolase, BktB, ELo1, Elovl-5, Elovl-6, ELOVL5, Elovl6, enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein, FadA, fatty acid elongase 5, KAT, KAT-1, KAT2, ketoacyl-CoA acyltransferase, ketoacyl-coenzyme A thiolase, long-chain 3-oxoacyl-CoA thiolase, mitochondrial 3-ketoacyl-CoA thiolase, oxoacyl-coenzyme A thiolase, PaaJ, pro-3-ketoacyl-CoA thiolase, PUFA elongase, SCP2/3-oxoacyl-CoA thiolase, SCPx, sterol carrier protein 2/3-oxoacyl-CoA thiolase, TFE-beta subunit, Thb, thiloase B, thiolase A, thiolase I, thiolase II, thiolase III

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.16 acetyl-CoA C-acyltransferase

Purification

Purification on EC 2.3.1.16 - acetyl-CoA C-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
general method for separation, quantitation and partial purification
multienzyme complex contains enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA epimerase and DELTA3-cis-DELTA-trans-enoyl-CoA isomerase
-
multienzyme complex: 3-hydroxyacyl-coenzyme A epimerase, cis-DELTA3-trans-DELTA2-enoyl-CoA isomerase, enoyl-CoA hydratase, L-3-hydroxyacyl-CoA thiolase
-
multienzyme complex: EC 4.2.1.17, EC 1.1.1.35, EC 5.3.3.3, EC 5.1.2.3, EC 2.3.1.16
native enzyme 27.2fold from liver mitochondria preparation by 5 steps of ion exchange and hydrophobic interaction chromatography
-
native enzyme 386.4fold to homogeneity by anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, and gel filtration
-
recombinant enzyme of KAT gene on chromosome 2 mutant
recombinant His-tagged truncated enzyme lacking the N-terminal peroxisomal targeting sequence of 34 amino acid residues from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, His-tag removal by thrombin, to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cation exchange chromatography, ultrafiltration, and gel filtration, tag cleavage by thrombin
recombinant tagged enzyme by affinity chromatography
-
single enzyme, no part of multienzyme complex of beta-oxidation cycle
-
thiolase A and B
-
trifunctional enzyme: 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/3-oxoacyl-CoA thiolase
-