2.3.1.46: homoserine O-succinyltransferase
This is an abbreviated version!
For detailed information about homoserine O-succinyltransferase, go to the full flat file.
Word Map on EC 2.3.1.46
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2.3.1.46
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chaperone
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acyltransferases
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refolding
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noncanonical
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cereus
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threonine
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biotechnology
- 2.3.1.46
- chaperone
- acyltransferases
-
refolding
-
noncanonical
- cereus
- threonine
- biotechnology
Reaction
Synonyms
homoserine O-succinyltransferase, homoserine O-transsuccinylase, homoserine succinyltransferase, homoserine transsuccinylase, homoserine-O-succinyltransferase, HST, HTS, MetA, MetA protein, succinyltransferase, homoserine
ECTree
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Purification
Purification on EC 2.3.1.46 - homoserine O-succinyltransferase
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Escherichia coli cells are lysed with 0.5 mg/ml lysozyme-1 mM phenylmethylsulfonyl fluoride-DNase I, sonicated, centrifuged, supernatants purified with Ni-nitrilo-triacetic acid agarose, gravity filtration of unbound protein, elution with 50 mM Tris-HCl, pH 7.5, NaCl, and imidazole, dialysis with potassium-phosphate buffer, pH 7.6
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recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2 different steps of anion exchange chromatography
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recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and dialysis to over 90% purity
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transformed Escherichia coli cells are lysed with 0.5 mg/ml lysozyme-1 mM phenylmethylsulfonyl fluoride-DNase I and sonicated, centrifuged, supernatants purified with Ni-nitrilo-triacetic acid agarose, gravity filtration of unbound protein, elution with 50 mM Tris-HCl, pH 7.5, NaCl, and imidazole, dialysis with potassium-phosphate buffer, pH 7.6
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