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2.3.2.13: protein-glutamine gamma-glutamyltransferase

This is an abbreviated version!
For detailed information about protein-glutamine gamma-glutamyltransferase, go to the full flat file.

Word Map on EC 2.3.2.13

Reaction

protein glutamine
+
alkylamine
=
protein N5-alkylglutamine
 +
NH3

Synonyms

AcTG-1, BmTGA, chloroplast transglutaminase, chlTGase, cold active transglutaminase, cold-active transglutaminase, EPB42, factor XIII, factor XIIIa, fibrin stabilizing factor, fibrinoligase, Galphah, glutamine:amine gamma-glutamyl-transferase, glutaminylpeptide gamma-glutamyltransferase, glutamyltransferase, glutaminylpeptide gamma-, gpTGase 2, gTG2, hfXIIIa, hTG2, hTGase 1, hTGase 2, hTGase 3, hTGase 6, KALB, KalbTG, KALB_7456, Laki-Lorand factor, mammalian transglutaminase, microbial transglutaminase, microbial transglutaminases, MsTGase, MTG, MTG-TX, MTGase, mTGase 2, OlTGT, plastidial transglutaminase, polyamine transglutaminase, protein 4.2, protein-glutamine gamma-glutamyltransferase, R-glutaminyl-peptide:amine gamma-glutamyl transferase, R-glutaminylpeptide-amine gamma-glutamyltransferase, SCTG, SMTG, STG I, t-TG, TG-2, TG1, TG2, TG3, TG4, TG5, TG6, TG7, tGA, TGase, TGase 1, TGase 2, Tgase 3, TGase 6, Tgase II, TGase-2, TGase2, TGB, TGK1, TGK2, TGL, TGM1, TGM2, TGM3, TGM4, TGM5, TGM6, TGM7, TGZ, tgz15, TGZo, tissue transglutaminase, tissue transglutaminase 2, tissue-TG, transglutaminase, transglutaminase 1, transglutaminase 2, transglutaminase 3, transglutaminase 6, transglutaminase C, transglutaminase factor XIII, transglutaminase type II, transglutaminase-2, transglutaminase-like protein, transglutaminase2, tTG, tTG-2, type 2 transglutaminase, type I transglutaminase, type-2 transglutaminase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.13 protein-glutamine gamma-glutamyltransferase

Storage Stability

Storage Stability on EC 2.3.2.13 - protein-glutamine gamma-glutamyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-14°C, presence of EDTA and 35% glycerol, pH 7-9, more than 85% remaining activity after several days
-20°C, concentrated guinea pig enzyme solutions, several months
-
-20°C, partially purified, 3 years
-
-20°C, Tgl-His6 protein remains stable for several months
-
-30°C, human zymogen factor XIII, over 1 year
-
-30°C, lyophilized human zymogen factor XIII, several years
-
-70°C, 6 months, little loss in activity
-
-70°C, concentrated enzyme solution, at least 3 months, no loss of activity
-
-80C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, several months, no loss of activity
0-4°C, presence of EDTA, pH 7-9, more than 85% remaining activity after several days
20°C, pH 6-8, cationic isozyme, long periods
-
4°C, 5 mM Tris-HCl/2 mM EDTA buffer, pH 7.5, up to 3 months
-
4°C, 50 mM Tris, pH 7.5, 1 mM EDTA/0.5 mM DTT, up to 2 weeks, no loss of activity
-
4°C, at least 3 months
-
4°C, at least 4 months
-
4°C, epidermal transglutaminase, at least 1 month
-
4°C, lyophilised purified enzyme, 1 week, 80% loss of activity
4°C, membrane preparation, rapid inactivation, DTT protects in combination with Ca2+ or EDTA
-
4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 1 week, 10% loss of activity
4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 2 weeks, 25% loss of activity
brain transglutaminase NI, 4°C, 2 weeks, complete loss of activity
-
commercial preparation of enzyme loses activity within hours of dissolution in various buffers at 0°C. Stabilization of enzyme by dissolving extract at 70 mU/min in 10 mM DTT and 1 mM diethylenetriaminepentaacetic acid, pH 7.0.
-
dilute enzyme solutions are unstable to storage at 4°C
-
follicle transglutaminase, -20°C, 3 months, no loss of activity
-
human factor XIIIa is more stable to storage, when Ca2+ is omitted
-