2.3.3.5: 2-methylcitrate synthase
This is an abbreviated version!
For detailed information about 2-methylcitrate synthase, go to the full flat file.
Word Map on EC 2.3.3.5
-
2.3.3.5
-
propionate
-
propionyl-coa
-
dehydratase
-
acetyl-coa
-
3-hydroxyvalerate
-
mcsa
-
methylisocitrate
-
polyhydroxyalkanoates
-
methylmalonyl-coa
-
propionyl
-
eutropha
-
halomonas
-
paracoccidioidomycosis
-
odd-chain
-
drug development
-
lutzii
-
paracoccidioides
-
coa-transferase
-
prpbcde
-
butyryl-coa
-
medicine
-
agriculture
- 2.3.3.5
- propionate
- propionyl-coa
- dehydratase
- acetyl-coa
- 3-hydroxyvalerate
- mcsa
- methylisocitrate
- polyhydroxyalkanoates
- methylmalonyl-coa
-
propionyl
- eutropha
- halomonas
-
paracoccidioidomycosis
-
odd-chain
- drug development
- lutzii
- paracoccidioides
-
coa-transferase
-
prpbcde
- butyryl-coa
- medicine
- agriculture
Reaction
Synonyms
2-MC synthase, 2-MCS, 2-MCS1, 2-methylcitrate oxaloacetate-lyase, 2-methylcitrate synthase 2, citrate synthase II, citrate synthase/2-methylcitrate synthase, EC 4.1.3.31, MCA condensing enzyme, MCS, Mcs1, mcsA, Methylcitrate synthase, methylcitrate synthetase, mmgD, MscA, PrpC, prpC2, Rv1131
ECTree
2.3.3.5 2-methylcitrate synthaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.3.3.5 - 2-methylcitrate synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + H2O + oxaloacetate
?
-
-
-
?
butyryl-CoA + H2O + oxaloacetate
?
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
-
-
ir
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16)
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16)
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
uses acetyl-CoA in preference to propionyl-CoA
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
uses acetyl-CoA in preference to propionyl-CoA
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
-
uses acetyl-CoA in preference to propionyl-CoA
-
-
?
n-butyryl-CoA + H2O + oxaloacetate
2-ethylcitrate + CoA
1.8% of the activity with propanoyl-CoA
-
-
?
n-butyryl-CoA + H2O + oxaloacetate
2-ethylcitrate + CoA
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
1.8% of the activity with propanoyl-CoA
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
the fungi-specific 2-methylcitrate cycle is responsible for detoxifying propionyl-CoA, a toxic metabolite produced as the fungus breaks down proteins and amino acids. The enzyme responsible for this detoxification is 2-methylcitrate synthase (mcsA) and is a potential candidate for the design of new anti-fungals
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16)
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
bifunctional enzyme with 2.3-fold higher activity as a 2-methylcitrate synthase (reaction of EC 2.3.3.16)
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
the enzyme is involved in the 2-methylcitrate cycle pathway which plays a significant role in the detoxification of propionyl-CoA in bacteria
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
the enzyme is specific to the methylcitrate cycle and essential for mycobacteria to grow on propionate as the sole carbon source
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
-
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview
-
-
ir
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
-
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
-
-
ir
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
implicated in the metabolism of propionate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
implicated in the metabolism of propionate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
implicated in the metabolism of propionate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
implicated in the metabolism of propionate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
implicated in the metabolism of propionate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
first key enzyme of the methylcitric acid cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
propanoyl-CoA is the most efficient substrate
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
-
first key enzyme of the methylcitric acid cycle
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
-
-
-
?
valeryl-CoA + H2O + oxaloacetate
?
3% of the activity with propanoyl-CoA
-
-
?
valeryl-CoA + H2O + oxaloacetate
?
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
3% of the activity with propanoyl-CoA
-
-
?
additional information
?
-
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
-
-
?
additional information
?
-
-
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
-
-
?
additional information
?
-
-
the enzyme also exhibits citrate synthase activity
-
-
?
additional information
?
-
-
the enzyme also exhibits citrate synthase activity
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is relatively specific for its acetyl-CoA substrate, utilizing propionyl-CoA and not acetyl-CoA
-
-
?
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
?
additional information
?
-
-
enzyme is relatively specific for its acetyl-CoA substrate, utilizing propionyl-CoA and not acetyl-CoA
-
-
?
additional information
?
-
-
enzyme is specific for oxaloacetate, does not catalyze liberation of CoA from acetyl- or propionyl-CoA in presence of glyoxylate, pyruvate or alpha-ketoglutarate, isobutyryl-CoA and isovaleryl-CoA are no substrates
-
-
?
