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2.4.2.10: orotate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about orotate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.10

Reaction

orotidine 5'-phosphate
+
diphosphate
=
Orotate
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

More, OMP synthase, OPRT, OPRTase, orotate phosphoribosyl pyrophosphate transferase, orotate phosphoribosyl transferase, orotate phosphoribosyltransferase, orotate PRTase, orotic acid phosphoribosyltransferase, orotidine 5'-monophosphate pyrophosphorylase, orotidine 5'-phosphate pyrophosphorylase, orotidine monophosphate pyrophosphorylase, orotidine phosphoribosyltransferase, orotidine-5'-phosphate pyrophosphorylase, orotidylate phosphoribosyltransferase, orotidylate pyrophosphorylase, orotidylic acid phosphorylase, orotidylic acid pyrophosphorylase, orotidylic phosphorylase, orotidylic pyrophosphorylase, phosphoribosyltransferase, orotate, Pyr5, PyrE, rv0382c, type I phosphoribosyltransferase, ura5

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.10 orotate phosphoribosyltransferase

Cloned

Cloned on EC 2.4.2.10 - orotate phosphoribosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells
-
expressed in COS-7 cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli JW3617(DE3) cells
expressed in Escherichia coli M15[pREP4]cells
expressed in Saccharomyces cerevisiae strain YGL268w
functional recombinant expression of the His-tagged chimeric fusion enzyme in Escherichia coli
His-tagged protein expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli BL21(DE3), gel filtration
-
into the pDONR221 vector, expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells
-
mutants are expressed in Escherichia coli strain CS101-4UI
OPRT, stable overexpression in the gastric cancer cell lines TMK-1 and MKN-45, enhancing the effect of 5-fluorouracil on the cells, transfected cells show a similar growth curve as the wild-type parent cells, while the drug sensitivity is increased compared to the untransfected cells, overview
-
structural gene pyrE
-
structural gene pyrE, subcloned from a genomic library in pBR328(pAV002), transduced into pyr-auxotroph Salmonella typhimurium strain SA2434 with phage P22, finally cloned to and expressed in overproducing strain Escherichia coli MB13
-