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0.025
5-Fluoroorotate
pH 8.0, 37°C
0.0016 - 10.01
5-phospho-alpha-D-ribose 1-diphosphate
0.19
Orotate methylester
-
-
0.03
orotidine 5'-monophosphate
-
-
0.0015 - 0.045
orotidine 5'-phosphate
1.59 - 3.84
Phosphonoacetic acid
additional information
additional information
-
0.0016
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.0079
5-phospho-alpha-D-ribose 1-diphosphate
pH and temperature not specified in the publication, recombinant multienzyme complex, OPRT activity
0.009
5-phospho-alpha-D-ribose 1-diphosphate
ordered mechanism, pH 8.0, 25°C
0.0093
5-phospho-alpha-D-ribose 1-diphosphate
pH and temperature not specified in the publication, recombinant bifunctional enzyme, OPRT activity
0.0113
5-phospho-alpha-D-ribose 1-diphosphate
37 °C, pH 8.0, native enzyme complex
0.016
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.0164
5-phospho-alpha-D-ribose 1-diphosphate
37 °C, pH 8.0, mixture of recombinant enzyme subunits
0.0282
5-phospho-alpha-D-ribose 1-diphosphate
pH 8.0, 37°C
0.0286
5-phospho-alpha-D-ribose 1-diphosphate
37 °C, pH 8.0, recombinant monofunctional enzyme
0.0286
5-phospho-alpha-D-ribose 1-diphosphate
pH and temperature not specified in the publication, recombinant monofunctional enzyme, OPRT activity
0.034
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.038 - 0.04
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.038 - 0.04
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type
0.041
5-phospho-alpha-D-ribose 1-diphosphate
wild type enzyme
0.043
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme D131A
0.044
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.062
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.064
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.086
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme D131A/D132A
0.096
5-phospho-alpha-D-ribose 1-diphosphate
ping-pong mechanism, pH 8.0, 25°C
0.12
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme D132A
0.36
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant
10.01
5-phospho-alpha-D-ribose 1-diphosphate
-
37°C, pH 8.0
0.013
diphosphate
-
wild-type
0.0143
diphosphate
pH 8.0, 37°C
0.016
diphosphate
ping-pong mechanism, pH 8.0, 25°C
0.096
diphosphate
-
phosphorolysis
0.22 - 0.25
diphosphate
-
-
0.22 - 0.25
diphosphate
-
-
0.002
Orotate
-
-
0.0045
Orotate
37 °C, pH 8.0, mixture of recombinant enzyme subunits
0.0056
Orotate
-
37°C, pH 8.0
0.0056
Orotate
37 °C, pH 8.0, native enzyme complex
0.007
Orotate
pH and temperature not specified in the publication, recombinant multienzyme complex, OPRT activity
0.0083
Orotate
pH and temperature not specified in the publication, recombinant bifunctional enzyme, OPRT activity
0.0094
Orotate
ordered mechanism, pH 8.0, 25°C
0.01
Orotate
ping-pong mechanism, pH 8.0, 25°C
0.0182
Orotate
pH 8.0, 37°C
0.0182
Orotate
37 °C, pH 8.0, recombinant monofunctional enzyme
0.0182
Orotate
pH and temperature not specified in the publication, recombinant monofunctional enzyme, OPRT activity
0.027 - 0.0275
Orotate
-
-
0.027 - 0.0275
Orotate
-
-
0.03 - 0.035
Orotate
-
wild-type
0.091
Orotidine
-
-
0.091
Orotidine
-
pH 8.0, 25°C
0.091
Orotidine
-
pH not specified in the publication, temperature not specified in the publication
0.096
Orotidine
-
pH 8.0, 25°C
0.096
Orotidine
-
pH not specified in the publication, temperature not specified in the publication
0.0015
orotidine 5'-phosphate
-
pH 8.0, 25°C
0.00155
orotidine 5'-phosphate
-
in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2
0.0016
orotidine 5'-phosphate
-
pH not specified in the publication, temperature not specified in the publication
0.00214
orotidine 5'-phosphate
-
in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2
0.0026
orotidine 5'-phosphate
ping-pong mechanism, pH 8.0, 25°C
0.0029
orotidine 5'-phosphate
-
in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2
0.0031 - 0.0036
orotidine 5'-phosphate
-
-
0.0031 - 0.0036
orotidine 5'-phosphate
-
wild-type
0.0037
orotidine 5'-phosphate
-
pH 8.0, 25°C
0.0037
orotidine 5'-phosphate
-
pH not specified in the publication, temperature not specified in the publication
0.00373
orotidine 5'-phosphate
-
in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2
0.004
orotidine 5'-phosphate
wild type enzyme
0.007
orotidine 5'-phosphate
mutant enzyme D131A
0.007
orotidine 5'-phosphate
mutant enzyme D132A
0.008 - 0.0083
orotidine 5'-phosphate
-
-
0.008 - 0.0083
orotidine 5'-phosphate
-
phosphorolysis
0.01
orotidine 5'-phosphate
mutant enzyme D131A/D132A
0.0251
orotidine 5'-phosphate
pH 8.0, 37°C
0.045
orotidine 5'-phosphate
-
-
1.59
Phosphonoacetic acid
-
-
3.84
Phosphonoacetic acid
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
principles of assay
-
additional information
additional information
-
principles of assay
-
additional information
additional information
-
HPLC microassay
-
additional information
additional information
-
HPLC microassay
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
detailed kinetic analysis, isothermal titration calorimetry, the double Theorell-Chance mechanism yields a steady-state rate equation indistinguishable in form from the observed classical ping-pong bi-bi kinetics, steady-state kinetics, overview
-