2.4.2.10: orotate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about orotate phosphoribosyltransferase, go to the full flat file.

Reaction

Synonyms

More, OMP synthase, OPRT, OPRTase, orotate phosphoribosyl pyrophosphate transferase, orotate phosphoribosyl transferase, orotate phosphoribosyltransferase, orotate PRTase, orotic acid phosphoribosyltransferase, orotidine 5'-monophosphate pyrophosphorylase, orotidine 5'-phosphate pyrophosphorylase, orotidine monophosphate pyrophosphorylase, orotidine phosphoribosyltransferase, orotidine-5'-phosphate pyrophosphorylase, orotidylate phosphoribosyltransferase, orotidylate pyrophosphorylase, orotidylic acid phosphorylase, orotidylic acid pyrophosphorylase, orotidylic phosphorylase, orotidylic pyrophosphorylase, phosphoribosyltransferase, orotate, Pyr5, PyrE, rv0382c, type I phosphoribosyltransferase, ura5

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.10 orotate phosphoribosyltransferase

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Results	in table
1	Activating Compound
44599	AA Sequence
10	Application
1	CAS Registry Number
28	Cloned(Commentary)
9	Crystallization (Commentary)
1113	Disease/ Diagnostics
6	Expression
4	General Information
9	General Stability
136	Inhibitors
18	kcat/KM [mM/s]
93	Ki Value [mM]
93	KM Value [mM]
12	Localization
40	Metals/Ions
35	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
94	Organism
8	Pathway
108	PDB ID
15	pH Optimum
4	pH Range
2	pH Stability
2	pI Value
12	Protein Variants
31	Purification (Commentary)
8	Reaction
1	Reaction Type
91	Reference
52	Source Tissue
30	Specific Activity [micromol/min/mg]
11	Storage Stability
103	Substrates and Products (Substrate)
28	Subunits
98	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
3	Temperature Stability [°C]
29	Turnover Number [1/s]

General Stability

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of the Enzyme Summary Page.

General Stability on EC 2.4.2.10 - orotate phosphoribosyltransferase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

0.05 mM UMP and 1% polyethyleneglycol stabilize during final stage of purification

Mus musculus

-

489713

5 mM Mg2+, 1 mM phosphoribose diphosphate and 2 mM DTT stabilize dilute enzyme solutions

Mus musculus

-

489712

dialysis against phosphate buffer, pH 7 or 7.5, unstable to, 2 mM DTT protect

Mus musculus

-

489712

dilution inactivates

Mus musculus

-

489712

native enzyme complex, stable for at least 6 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 300 mM NaCl, 5 mM dithiothreitol, 20% glycerol. At 4°C, loss of 50% of activity within 4 weeks.

Plasmodium falciparum

Q8T6J6

658105

phosphate, 0.3 M and above, stabilizes

Mus musculus

-

489712

proteins, e.g. albumin, do not stabilize dilute enzyme solutions

Mus musculus

-

489712

recombinant enzyme dimer, stable for at least 3 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 5 mM dithiothreitol, 20% glycerol. In absence of dithiothreitol and glycerol, loss of 50% of activity during overnight storage at 4°C.

Plasmodium falciparum

Q8T6J6

658105

recombinant enzyme, stable for at least 3 months at 4°C or -20°C in presence of 1 mM dithiothreitol and 10% glycerol

Plasmodium falciparum

Q8N0R1

659903