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2.5.1.21: squalene synthase

This is an abbreviated version!
For detailed information about squalene synthase, go to the full flat file.

Word Map on EC 2.5.1.21

Reaction

2 (2E,6E)-farnesyl diphosphate +

NAD(P)H
+
H+
=
squalene
 + 2 diphosphate +
NAD(P)+

Synonyms

BbSS, BSS, CrSQS, dt-ySQase, Erg9, EtSS, farnesyl-diphosphate farnesyltransferase, farnesyl-diphosphate:farnesyldiphosphate farnesyltransferase, farnesyldiphosphate farnesyltransferase 1, farnesyldiphosphate:farnesyldiphosphate farnesyltransferase, farnesyltransferase, FDFT1, hSQS, presqualene synthase, presqualene-diphosphate synthase, SgSQS, SQase, SQS, SQS1, SQS2, squalene synthase, squalene synthase 1, squalene synthase 2, squalene synthetase, SS1, SSase, SSN, synthase, squalene, TkSQS1, TkSQS2

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.21 squalene synthase

Crystallization

Crystallization on EC 2.5.1.21 - squalene synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with different inhibitors
mutant with deletion of 47 C-terminal amino acids
purified recombinant truncated detagged SQS(31-370) in complex with inhibitor zaragozic acid A, vapour diffusion, mixing an equal volume of protein solution containing 15 mg/ml protein with precipitating solution containing 20% PEG2K-MME, 0.01 M NiCl2, 0.1 M Tris, pH 8.5, 1.4 M sodium citrate tribasic dehydrate, 0.1 M Na-HEPES, pH 7.5, 2 M K2HPO4/NaH2HPO4, pH 6.5, room temperature, X-ray diffraction structure determination and analysis, molecular replacement
-
purified recombinant wild-type and mutant enzymes in complex with Mg2+ and/or farnesyl diphosphate or presqualene diphosphate, respectively, mixing equal volumes of protein solution, containing 10 mg/ml protein and 3 mM substrate or 1 mM intermediate, with or without 1 mM Mg2+, with precipitant solution (form I: 20% PEG 2000 MME, 0.01 M NiCl2, 0.1 M Tris, pH 8.5 and form II: 1.4 M sodium citrate tribasic dehydrate, 0.1 M Na HEPES, pH 7.5; form III, 2 M K2HPO4/NaH2PO4, pH 6.5) at 25°C, X-ray diffraction structure determination and analysis at 1.75-2.35 A resolution, modelling