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2.6.1.1: aspartate transaminase

This is an abbreviated version!
For detailed information about aspartate transaminase, go to the full flat file.

Word Map on EC 2.6.1.1

Reaction

L-aspartate
+
2-oxoglutarate
=
oxaloacetate
 +
L-glutamate

Synonyms

2-oxoglutarate-glutamate aminotransferase, AAT, AAT-2, AAT-3, AAT3, aatA, AATase, aatB3, all2340, alr1039, alr2765, alr4853, aminotransferase, aspartate, aoa/coa, AsAT, Asp AT, aspartate alpha-ketoglutarate transaminase, aspartate aminotransferase, aspartate aminotransferase 1, aspartate aminotransferase A, aspartate AT, aspartate transaminase, aspartate, 2-oxoglutarate aminotransferase, aspartate-2-oxoglutarate transaminase, aspartate/(R)-cysteate:2-oxoglutarate aminotransferase, aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase, aspartate:2-oxoglutarate amino-transferase, aspartate:2-oxoglutarate aminotransferase, aspartic acid aminotransferase, aspartic aminotransferase, aspartyl aminotransferase, AspAT, AspATSs, aspB, aspC, AspT, AST, AST-Bb, AtPAT, bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase, C-S lyase, CAA1, cAST, Cgl0240, class Ibeta AAT, EcAspAT, GL50803_91056, glutamate oxaloacetate transaminase, glutamate oxaloacetate transaminase 1, glutamate-oxalacetate aminotransferase, glutamate-oxalate transaminase, glutamate-oxaloacetate transaminase 1, glutamic oxalic transaminase, glutamic oxaloacetic transaminase, glutamic-aspartic aminotransferase, glutamic-aspartic transaminase, glutamic-oxalacetic transaminase, glutamic-oxaloacetic transaminase, GOT, GOT (enzyme), GOT1, GOT1L1, GOT2, KAT IV, L-aspartate aminotransferase, L-aspartate transaminase, L-aspartate-2-ketoglutarate aminotransferase, L-aspartate-2-oxoglutarate aminotransferase, L-aspartate-2-oxoglutarate-transaminase, L-aspartate-alpha-ketoglutarate transaminase, L-aspartate:2-oxoglutarate aminotransferase, L-aspartateartate aminotransferase, L-aspartic aminotransferase, L-AspAT, mitAAT, More, oxaloacetate transferase, oxaloacetate-aspartate aminotransferase, Pat, PfAspAT, plastid aspartate aminotransferase, prephenate aminotransferase, protein TT0402, PT-AAT, Rv3722c, Sar2028, SsAspAT, Tb11.02.2740, transaminase A

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.1 aspartate transaminase

General Stability

General Stability on EC 2.6.1.1 - aspartate transaminase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-oxoglutarate does not protect against heat inactivation
-
2-oxoglutarate stabilizes against thermal inactivation
35% decrease in activity after 5 days under the influence of 50 mM D-fructose, 46% decrease in activity under the influence of 500 mM D-fructose.The enzyme undergoes glycation when incubated with D-fructose leading to a shift down of pI. Uric acid has a protective effect against late glycation
-
addition of glycerol, DTT and pyridoxal 5'-phosphate to buffers, required for stabilization during purification
-
aspartate, 10 mM, or pyridoxal 5'-phosphate, 0.2 mM protects against heat inactivation
-
AspATSs, when incubated at 37°C in the presence of trypsin, is cleaved after Lys32 and Lys33 and looses its activity. At 37°C, substrates are not able to protect the enzyme from proteolysis, while at 75°C the presence of substrates lowers the inactivation rate about 4fold
complete loss of activity after freezing and thawing
-
cysteinic acid protects the cytosolic isoform against thermal denaturation, not aspartate or 2-oxoglutarate
-
enzyme is stabilized by the intramitochondrial chaperone homologues GroEL and GroES
-
inactivation at 1.3 M NaCl and KCl
-
labile enzyme is stabilized by pyridoxyl 5'-phosphate and L-aspartate
-
presence of substrates renders AspATSs less sensitive to thermolysin, possibly by inducing a conformational transition