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2.6.1.19: 4-aminobutyrate-2-oxoglutarate transaminase

This is an abbreviated version!
For detailed information about 4-aminobutyrate-2-oxoglutarate transaminase, go to the full flat file.

Word Map on EC 2.6.1.19

Reaction

4-aminobutanoate
+
2-oxoglutarate
=
succinate semialdehyde
 +
L-glutamate

Synonyms

4-aminobutyrate aminotransferase, 4-aminobutyrate transaminase, 4-aminobutyrate-2-ketoglutarate aminotransferase, 4-aminobutyrate-2-oxoglutarate aminotransferase, 4-aminobutyrate-2-oxoglutarate transaminase, 4-aminobutyric acid 2-ketoglutaric acid aminotransferase, 4-aminobutyric acid aminotransferase, ABAT, aminobutyrate aminotransferase, aminobutyrate transaminase, aminotransferase, aminobutyrate, argD, argD6803, argD7002, Atu3000, beta-alanine aminotransferase, beta-alanine transaminase, beta-alanine-oxoglutarate aminotransferase, beta-alanine-oxoglutarate transaminase, bioA, CG010_026395, CgGABA-AT, GABA aminotransferase, GABA transaminase, GABA transferase, GABA-2-oxoglutarate aminotransferase, GABA-2-oxoglutarate transaminase, GABA-alpha-ketoglutarate aminotransferase, GABA-alpha-ketoglutarate transaminase, GABA-alpha-ketoglutaric acid transaminase, GABA-alpha-oxoglutarate aminotransferase, GABA-AT, GABA-oxoglutarate aminotransferase, GABA-oxoglutarate transaminase, GABA-T, GABA-T1, GABA-T2, GABA-T3, GABA-TA, GABA-transaminase, GABA:2-oxoglutarate aminotransferase, GABAT, GabT, gamma-aminobutyrate aminotransaminase, gamma-aminobutyrate aminotransferase, gamma-aminobutyrate transaminase, gamma-aminobutyrate transaminases, gamma-aminobutyrate-alpha-ketoglutarate aminotransferase, gamma-aminobutyrate-alpha-ketoglutarate transaminase, gamma-aminobutyrate: GABA transaminase, gamma-aminobutyrate:alpha-oxoglutarate aminotransferase, gamma-aminobutyric acid aminotransferase, gamma-aminobutyric acid pyruvate transaminase, gamma-aminobutyric acid transaminase, gamma-aminobutyric acid-2-oxoglutarate transaminase, gamma-aminobutyric acid-alpha-ketoglutarate transaminase, gamma-aminobutyric acid-alpha-ketoglutaric acid aminotransferase, gamma-aminobutyric transaminase, gatT, glutamate-succinic semialdehyde transaminase, homotaurine:2-oxoglutarate aminotransferase, MdGABA-T1, MdGABA-T2, More, NCgl0462, NCgl2515, Osl2, pollen-pistil incompatibility 2, Pop2, SkUga1p, slr1022

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.19 4-aminobutyrate-2-oxoglutarate transaminase

Cloned

Cloned on EC 2.6.1.19 - 4-aminobutyrate-2-oxoglutarate transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
brain cDNA, expressed in Escherichia coli strain BL21(DE3)pLysS transformed with expression vector pETG1.5
-
cDNA clone isolation and sequence determination
-
co-expression of the truncated GABA-T cDNA with the GroES/ELmolecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells, and GABA-T is expressed in Nicotiana tabacum BY-2 cells
expressed in Escherichia coli BL-21(DE3) cells
expressed in Escherichia coli strain XL1-Blue
-
expression as His-tagged protein
expression in Escherichia coli
-
full-length GABA-T1 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T1 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells
full-length GABA-T2 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T2 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells
full-length GABA-T3 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T3 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells
gene ABAT, genetic regulation, quantitative reverse transcription and quantitative reverse PCR enzyme expression analysis in bone marrow samples, and coexpression analsis of of lncRNAs (DELs) and mRNAs (DEMs)
gene argD, functional recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain DH5alpha
gene Cgl0479, DNA and amino acid sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)-T1R
gene davD, recombinant expression in Corynebacterium glutamicum strain KCTC 1857, coexpression with 5-aminovalerate transaminase (davT, EC 2.6.1.48) from Pseudomonas putida, glutarate semialdehyde dehydrogenase (gabT, EC 1.2.1.20) from Pseudomonas putida, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida
gene ectB, recombinant overexpression of codon-optimized version of (Pl)ectB as N-terminally Strep II-tagged protein from modified plasmid pLC52 in Escherichia coli strain BL21 and TOP10 cells
gene gabT, recombinant expression in Agrobacterium tumefaciens strain C58
gene gabT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene NCgl2515, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex