1.14.11.11: hyoscyamine (6S)-dioxygenase

This is an abbreviated version!
For detailed information about hyoscyamine (6S)-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.11

Reaction

L-hyoscyamine
+
2-oxoglutarate
+
O2
=
(6S)-hydroxyhyoscyamine
+
succinate
+
CO2

Synonyms

6H6, AaH6H, AtH6H, H6H, HnH6H, hyoscyamine 6-hydroxylase, hyoscyamine 6beta-hydroxylase, hyoscyamine-6beta-hydroxylase, oxygenase, hyoscyamine 6beta-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.11 hyoscyamine (6S)-dioxygenase

Engineering

Engineering on EC 1.14.11.11 - hyoscyamine (6S)-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K97A
the shows a 3.3fold improved hydroxylation activity compared with the wild-type enzyme
K97E
the shows a 2.7fold improved hydroxylation activity compared with the wild-type enzyme
K97G
the shows a 1.7fold improved hydroxylation activity compared with the wild-type enzyme
S14H
the shows a 2.4fold improved hydroxylation activity compared with the wild-type enzyme
S14P
the shows a 3.7fold improved hydroxylation activity compared with the wild-type enzyme
S14P/K97A
double mutant shows a 4.4fold improved hydroxylation activity compared with the wild-type enzyme, and the in vivo epoxidation activity is also improved by 2.3times. With this mutant, in a 5 l bioreactor with a working volume of 3 l, scopolamine is produced via a single-enzyme-mediated two-step transformation from hyoscyamine of 500 mg/l in 97% yield (1.068 g)
D219H
-
inactive mutant enzyme
D219N
-
inactive mutant enzyme
H217Q
-
inactive mutant enzyme
H66Q
-
mutant enzyme has 97% of the activity of the wild-type enzyme
S274Q
-
inactive mutant enzyme
additional information