Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.29: 4-nitrophenol 2-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.29

Reaction

4-nitrophenol
+
NADH
 +
H+
 +
O2
=
4-nitrocatechol
 +
NAD+
 +
H2O

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.29 4-nitrophenol 2-monooxygenase

Engineering

Engineering on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H293A
M192I
-
site-directed mutagenesis, the mutant shows significantly reduced activity compared to wild-type enzyme
N450A
R100A
R370A
V292A
-
site-directed mutagenesis, the mutant shows significantly reduced activity compared to wild-type enzyme
V292L
-
site-directed mutagenesis, the mutant shows slightly reduced activity compared to wild-type enzyme
H293A
-
complete loss of activity
-
R100A
R370A
H293A
-
complete loss of activity
-
R100A
R370A