1.2.1.13: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating), go to the full flat file.

Word Map on EC 1.2.1.13

Reaction

D-glyceraldehyde 3-phosphate
+
phosphate
+
NADP+
=
3-phospho-D-glyceroyl phosphate
+
NADPH
+
H+

Synonyms

A2B2-GAPDH, A2B2-glyceraldehyde-3-phosphate dehydrogenase, A4 glyceraldehyde 3-phosphate dehydrogenase, A4-GAPDH, D-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating), GapA, GapA-1, GapB, GAPC, GAPD, GAPDH, GAPDH (A4), glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating), glyceraldehyde-3-dehydrogenase, glyceraldehyde-3-P dehydrogenase, glyceraldehyde-3-phosphate dehhydrogenase (NADP+) (phoshphorylating), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), glyceraldehyde-P dehydrogenase, GraP-DH, NAD(P)-GAPDH, NADP(H)-glyceraldehyde-3-phosphate dehydrogenase, NADP+-dependent G-3-P dehydrogenase, NADP+-dependent gapB, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent GAPDH, NADP-dependent glyceradehdye-3-phosphate dehydrogenase, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP-dependent glyceraldehyde phosphate dehydrogenase, NADP-dependent glyceraldehydephosphate dehydrogenase, NADP-GAPDH, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADP-GPD, NADP-triose phosphate dehydrogenase, NADPH-D-GA3P, NADPH-glyceraldehyde-3-phosphate dehydrogenase, NAPD-linked glyceraldehyde-3-P dehydrogenase, Ov-GAPDH, phosphorylating GAP dehydrogenase, phosphorylating glyceraldehyde-3-phosphate dehydrogenase, photosynthetic GAPDH, SSO0528, triosephosphate dehydrogenase (NADP+)

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.13 glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

Inhibitors

Inhibitors on EC 1.2.1.13 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-phospho-D-glyceroyl phosphate
-
-
6-Hydroxydopamine
-
-
chloroplast protein CP12
-
the chloroplast protein CP12 behaves as a negative regulator of GAPDH activity
-
Cu2+
-
inhibition is reversed by EDTA
cystamine
-
inhibitory when the enzyme is activated by effectors
DL-glyceraldehyde 3-phosphate
-
-
GAPDH segregator
-
H2O2
-
rapid reversible deactivation of untreated and GSH-treated enzyme preparation, GSH reverses the inhibition
iodoacetamide
iodoacetate
-
preincubation with glyceraldehyde-phosphate, 10fold molar excess over iodoacetate, prevents inactivation
NADH
-
strong inhibition with excess co-substrate s observed and no activity is observed with over 2 mM NADH
NADPH
sulfite
-
non-competitive inhibitor with respect to both NADPH and 3-phosphoglycerate
thioredoxin
-
a regulatory disulfide between Cys359 and Cys358 of the C-terminal extension of GapB does form in presence of oxidized thioredoxin. This covalent modification is required for the NAD+-dependent association into higher oligomers and inhibition of the NADPH-dependent activity
additional information
-
the activity of the GAPDH-CP12 complex is not affected by addition of bovine serum albumin, Rubisco, EC 4.1.1.39, or phosphoribulokinase, PRK EC 2.7.1.19. Incubation of GAPDH alone with NADPH, DTT or NADP has no effect on its activity
-