glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

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For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating), go to the full flat file.

Word Map on EC


D-glyceraldehyde 3-phosphate
3-phospho-D-glyceroyl phosphate


A2B2-GAPDH, A2B2-glyceraldehyde-3-phosphate dehydrogenase, A4 glyceraldehyde 3-phosphate dehydrogenase, A4-GAPDH, D-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating), GapA, GapA-1, GapB, GAPC, GAPD, GAPDH, GAPDH (A4), glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating), glyceraldehyde-3-dehydrogenase, glyceraldehyde-3-P dehydrogenase, glyceraldehyde-3-phosphate dehhydrogenase (NADP+) (phoshphorylating), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), glyceraldehyde-P dehydrogenase, GraP-DH, NAD(P)-GAPDH, NADP(H)-glyceraldehyde-3-phosphate dehydrogenase, NADP+-dependent G-3-P dehydrogenase, NADP+-dependent gapB, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent GAPDH, NADP-dependent glyceradehdye-3-phosphate dehydrogenase, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP-dependent glyceraldehyde phosphate dehydrogenase, NADP-dependent glyceraldehydephosphate dehydrogenase, NADP-GAPDH, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADP-GPD, NADP-triose phosphate dehydrogenase, NADPH-D-GA3P, NADPH-glyceraldehyde-3-phosphate dehydrogenase, NAPD-linked glyceraldehyde-3-P dehydrogenase, Ov-GAPDH, phosphorylating GAP dehydrogenase, phosphorylating glyceraldehyde-3-phosphate dehydrogenase, photosynthetic GAPDH, SSO0528, triosephosphate dehydrogenase (NADP+)


     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
       glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)


Purification on EC - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

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2 isoenzymes: 1 and 2
3 enzyme forms: GAPDH I, GAPDH II, GAPDH III
by Ni2+ chelate chromatography and pH-shift under denaturating conditions
cell-free preparations for measuring the enzyme activities are obtained
DEAE Trisacryl column chromatography
heat treatment at 80°C is an effective first step in the purification of these recombinant enzymes from extracts of the Escherichia coli host
HisTrap column chromatography and Superdex 200 gel filtration
native and recombinant enzyme
purified from spinach leaves
recombinant and native GAPDH are purified to apparent homogeneity from Escherichia coli cells and Chlamydomonas reinhardtii, respectively
the GAPDH–CP12–FNR complex is purified using ion exchange chromatography and gel filtration, followed by complex dissociation with 20 mM DTT for 20 min at 30°C, and gel filtration to about 86% purity, all steps in presence of NADPH
using a Q-Sepharose HP and a MonoQ anion-exchange column