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1.7.1.B1: xenobiotic reductase B

This is an abbreviated version!
For detailed information about xenobiotic reductase B, go to the full flat file.

Wordmap for 1.7.1.B1

Word Map on EC 1.7.1.B1 Wordmap for 1.7.1.B1

Reaction

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2,4,6-trinitrotoluene
+ 2 NADPH + 2 H+ =
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N-hydroxy-2-methyl-3,5-dinitroaniline
+ 2 NADP+ +
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H2O

Synonyms

flavoprotein reductase, old yellow enzyme, OYE, XenA, XenB, XenB protein, xenobiotic reductase A, xenobiotic reductase B, xenobiotic reductase B (XenB)-like flavin reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                EC 1.7.1.B11.7.1.B1 xenobiotic reductase B

Cloned

Cloned on EC 1.7.1.B1 - xenobiotic reductase B

for references in articles please use BRENDA:EC1.7.1.B1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta pLysS cells
-
For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS.
-
xenB gene is amplified using appropriate primers with BamHI and HindIII sites and Pseudomonas putida KT2440 chromosomal DNA as a template. After digestion with restriction enzymes, the PCR product is ligated into the pET28b(+) vector. Resulting plasmid contains the coding sequence in frame with a DNA sequence encoding a His-tag, which resulted in a hexahistidine tail. For protein-His6 expression, plasmid is transformed into Escherichia coli BL21.