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EC 3.1.21.2 Details
show Brenda entryEC number
3.1.21.2
Accepted name
deoxyribonuclease IV
Reaction
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5′-phosphooligonucleotide end-products
Other name(s)
deoxyribonuclease IV (phage-T4-induced) (misleading),
endodeoxyribonuclease IV (phage T4-induced) (misleading),
E. coli endonuclease IV,
endodeoxyribonuclease (misleading),
redoxyendonuclease,
deoxriboendonuclease (misleading),
endonuclease II,
endonuclease IV,
DNA-adenine-transferase,
nfo (gene name)
CAS registry number
63363-78-0
Comment
The enzyme is an apurinic/apyrimidinic (AP) site endonuclease that primes DNA repair synthesis at AP sites. It specifically cleaves the DNA backbone at AP sites and also removes 3′ DNA-blocking groups such as 3′ phosphates, 3′ phosphoglycolates, and 3′ α,β-unsaturated aldehydes that arise from oxidative base damage and the activity of combined glycosylase/lyase enzymes. It is also the only known repair enzyme that is able to cleave the DNA backbone 5′ of the oxidative lesion α-deoxyadenosine. The enzyme has a strong preference for single-stranded DNA.
History
created 1972 as EC 3.1.4.30, transferred 1978 to EC 3.1.21.2, modified 2014
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1 Oxidoreductases
3 Hydrolases
3.1.24.1 created 1978, modified 1982, deleted 1984 3.1.24.2 created 1978, modified 1982, deleted 1984 3.1.24.3 created 1978, modified 1982, deleted 1984 3.1.24.4 created 1978, modified 1982, deleted 1984
