Information on EC 1.1.1.380 - L-gulonate 5-dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.380
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RECOMMENDED NAME
GeneOntology No.
L-gulonate 5-dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-gulonate + NAD+ = D-fructuronate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-gulonate degradation
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Pentose and glucuronate interconversions
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
L-gulonate:NAD+ 5-oxidoreductase
The enzyme, characterized from the bacterium Halomonas elongata, participates in a pathway for L-gulonate degradation.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
YjjN belongs to the zinc-containing alcohol dehydrogenase family. This protein family contains dimeric or tetrameric proteins that coordinate two zinc atoms per subunit. Four conserved cysteine residues, that coordinate the zinc atoms and an aspartate residue predicting NAD+ specificity are found from the YjjN sequence (C92, C95, C98, C106, and D193)
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-galactonate + NAD+
D-tagaturonate + NADH + H+
show the reaction diagram
preferred substrate
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?
L-gulonate + NAD+
D-fructuronate + NADH + H+
show the reaction diagram
additional information
?
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enzyme YjjN is an L-galactonate 5-dehydrogenase having activity also for L-gulonate. Reaction product identification by GC/MS analysis. No activity with substrates of similar structure, L-fuconate and L-lyxonate. And no activity with L-mannonate, L-rhamnonate, D-gulonate, D-galacturonate, D-glucuronate, D-gluconate, D-lyxonate, D-xylonate, D-ribonate, D-arabonate, D-mannonate, D-erythronate, D-glucose, D-xylitol, D-sorbitol, glucooctanic acid, and meso-galactarate. The hydroxyl group at the C6 seems to be essential for the activity
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-galactonate + NAD+
D-tagaturonate + NADH + H+
show the reaction diagram
Q8XB60
preferred substrate
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?
L-gulonate + NAD+
D-fructuronate + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no activity with NADP+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
required, zinc enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
L-gulonate
pH 7.9, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
L-gulonate
pH 7.9, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
the optimum pH for the activity with L-galactonate is pH 8.0 while the remaining activity at pH 7.0, pH 7.5, and pH 9.0 is 73%, 97%, and 0%, respectively
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
the strain O157:H7 shows the ability to grow on L-galactonate as a sole carbon source
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, purified recombinant His-tagged enzyme, stability of enzyme YjjN seems to be rather poor since the activity of purified protein decreases dramatically when stored at 4°C overnight
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DH3) by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene yjjN, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DH3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcription of yjjN is upregulated during growth on L-galactonate
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
deletion of the gene results in a strain with the impaired ability to grow on L-galactonate as a sole carbon source
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
YjjN can be applied for a quantitative L-galactonate and L-gulonate detection in a coupled reaction with diaphorase