EC Number |
Reference |
---|
7.1.1.1 | DEAE-Sepharose column chromatography and hydroxyapatite column chromatography |
392594 |
7.1.1.1 | - |
392598, 392618, 392674, 658003, 658022, 658197, 659274, 674194, 675352, 733378, 742240 |
7.1.1.1 | the recombinant enzyme is purified by pre-extraction of the membranes with sodium cholate and Triton X-100, solubilization of the enzyme with sodium deoxycholate in the presence of 1 M potassium chloride, and centrifugation through a 1.1 M sucrose solution. The wild type enzyme is purified by phenyl Sepharose column chromatography, DEAE-Bio-Gel A column chromatography, and NAD+ agarose column chromatography |
392618 |
7.1.1.1 | His-tagged H91E mutant enzyme |
392664 |
7.1.1.1 | recombinant domain I |
392665, 392667 |
7.1.1.1 | recombinant domains I and III |
392665 |
7.1.1.1 | recombinant wild-type, T393C, R425C, G430C and A432C mutant domain III |
392667 |
7.1.1.1 | recombinant domain I and recombinant E155W and Y171W mutant domain III |
392674 |
7.1.1.1 | recombinant protein |
657468, 658003 |
7.1.1.1 | recombinant proteins |
657960 |