1.10.3.1: catechol oxidase

This is an abbreviated version!
For detailed information about catechol oxidase, go to the full flat file.

Reaction

2 catechol +

Synonyms

1,2-benzene: oxygen oxidoreductase, 1,2-benzenediol:oxygen oxidoreductase, AoCO4, catalase-phenol oxidase, catechol oxidase, catecholase, catecholoxidase, CATPO, CO, copper-S100B, cresolase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, germin-like protein, GLP, hemocyanin, ibCO, LsPPO, mettyrosinase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:oxygen oxidoreductase, More, MppO, o-diphenol oxidoreductase, o-diphenol: dioxygen oxidoreductase, dehydrogenating, o-diphenol:oxygen oxidoreductase, o-diphenolase, o-diphenoloxidase, oxytyrosinase, phenol oxidase, phenolase, phenoloxidase, polyphenol oxidase, polyphenoloxidase, PPO, PPO 1, PPO 2, PPO II, PPO-6, pyrocatechol oxidase, TYR3, tyrosinase

ECTree

     1 Oxidoreductases

         1.10 Acting on diphenols and related substances as donors

             1.10.3 With oxygen as acceptor

                1.10.3.1 catechol oxidase

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Results	in table
40	Activating Compound
306	AA Sequence
11	Application
1	CAS Registry Number
10	Cloned(Commentary)
9	Cofactor
7	Crystallization (Commentary)
2100	Disease/ Diagnostics
2	Expression
13	General Information
2	General Stability
113	IC50 Value
459	Inhibitors
33	Ki Value [mM]
185	KM Value [mM]
14	Localization
58	Metals/Ions
77	Molecular Weight [Da]
59	Natural Substrates/ Products (Substrates)
123	Organism
5	Pathway
14	PDB ID
86	pH Optimum
25	pH Range
20	pH Stability
2	pI Value
10	Posttranslational Modification
1	Protein Variants
54	Purification (Commentary)
3	Reaction
3	Reaction Type
118	Reference
1	Renatured (Commentary)
93	Source Tissue
120	Specific Activity [micromol/min/mg]
2	Storage Stability
503	Substrates and Products (Substrate)
49	Subunits
251	Synonyms
1	Systematic Name
65	Temperature Optimum [°C]
19	Temperature Range [°C]
55	Temperature Stability [°C]
71	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.10.3.1 - catechol oxidase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 40

Solanum tuberosum

-

quite stable, purified enzyme

673783

20 - 30

2 entries

20.8

Physalis peruviana

-

kinetic parameters for the thermal inactivation of Cape gooseberry PPO, overview

744955

25 - 75

Citrus unshiu

-

most stable at 25-45°C, temperature-activity profile overview

744221

30

2 entries

35

3 entries

4 - 100

Cynara cardunculus var. scolymus

-

the enzyme is stable at 4°C for 240 min, but is unstable at 75 and 100°C. Heat treatment at 100°C from 20 to 80 min decreases enzyme activity (62.5%)

712219

40

4 entries

40 - 50

Malus domestica

-

24 h, purified enzyme, over 50% activity remaining

675167

45

Malus sp.

-

30 min, purified enzyme, stable

673785

50

6 entries

55

4 entries

57

Mauritia flexuosa

-

crude enzyme extract, loss of 11.57% activity after 20 min

744956

60

5 entries

60 - 75

Musa x paradisiaca

-

half-life values of the enzyme range from 17 to 77 min

713387

65

Fragaria x ananassa

-

10 min, partially purified enzyme, almost complete inactivation

675166

67

Mauritia flexuosa

-

crude enzyme extract, loss of about 50% activity after 20 min

744956

70

5 entries

75

2 entries

77

Mauritia flexuosa

-

crude enzyme extract, loss of 59.93% activity after 20 min

744956

80

5 entries

90

Raphanus sativus

-

complete inactivation after 2.5 min

745732

additional information

5 entries

20 - 30

Gossypium hirsutum

-

60 min

701760

20 - 30

Malus domestica

-

72 h, purified enzyme, over 50% activity remaining

675167

30

Ferula sp.

-

60 min, purified enzyme, 40% reduced activity

673779

30

Trichoderma reesei

-

purified recombinant enzyme, half-life: 18 h

673560

35

Alternaria alternata

-

stable below 35°C, 50% inactivation after 30 min at 44°C, almost complete loss of activity at 60°C

440428

35

Cynara cardunculus var. scolymus

-

60 min, about 5% loss of activity with pyrogallol. 60 min, about 10% loss of activity with 4-methylcatechol. 60 min, activity with catechol is stable

658960

35

Pouteria sapota

-

PPO is maximum thermally stable up to 35°C

713239

40

Ferula sp.

-

60 min, purified enzyme, 65% reduced activity

673779

40

Lablab purpureus

-

above, loss in activity

671857

40

Lonicera japonica

-

the residual soluble enzyme activity decreases significantly when temperature reaches 40°C

727570

40

Trichoderma reesei

-

purified recombinant enzyme, half-life: 3,75 h

673560

50

Aspergillus oryzae

-

half-live 20 h at 50°C

701876

50

Coffea arabica

-

leaf enzyme, no loss of activity after 30 min, 50% loss of activity after 5 min at 80°C, endosperm enzyme, 20% loss of activity after 30 min at 50°C, 50% loss of activity after 2.5 min at 80°C

440436

50

Ferula sp.

-

60 min, purified enzyme, complete inactivation

673779

50

Malus sp.

-

30 min, purified enzyme, loss of 50% activity

673785

50

Morus alba

-

50 min, 50% inactivation

658788

50

Trichoderma reesei

-

purified recombinant enzyme, half-life: 15 min

673560

55

Cynara cardunculus var. scolymus

-

60 min, about 30% loss of activity with pyrogallol and 4-methylcatechol. 60 min, activity with catechol is stable

658960

55

Fragaria x ananassa

-

10 min, partially purified enzyme, loss of 50% activity

675166

55

Ocimum basilicum

-

30 min, purified enzyme, loss of 50% activity

674089

55

Prunus persica

-

half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55°C, half-life at 76°C: 2.2 min

440426

60

Aspergillus oryzae

-

enzyme shows thermostability up to 60°C, half-live 2 h at 60°C

701876

60

Ferula sp.

-

40 min, purified enzyme, irreversible denaturation

673779

60

Malus domestica

-

1 h, purified enzyme, over 50% activity remaining

675167

60

Morus alba

-

50 min, 30% inactivation

658788

60

Musa x paradisiaca

-

peel enzyme, loss of 10% activity after 30 min

440437

70

Castanea henryi

-

30 min, 92% loss of activity

659821

70

Malus domestica

-

15 min, purified enzyme, over 50% activity remaining

675167

70

Mangifera indica

D2XZ13

30 min, 50% activity remaining

744952

70

Musa x paradisiaca

-

pulp enzyme, loss of 20% activity after 10 min

440433

70

Solanum tuberosum

-

almost complete inactivation within 30 min, purified enzyme

673783

75

Cynara cardunculus var. scolymus

-

40 min, complete loss of activity with pyrogallol and 4-methylcatechol. 50 min, about 60% loss of activity with catechol, complete inactivation after 60 min

658960

75

Ocimum basilicum

-

30 min, purified enzyme, loss of 55%, 80%, and 70% activity with substrates 4-methylcatechol, catechol, and pyrogallol, respectively

674089

80

Malus domestica

-

15 min, purified enzyme, inactivation

675167

80

Morus alba

-

50 min, 20% inactivation

658788

80

Raphanus sativus

-

complete inactivation after 3.5 min

745732

80

Urtica dioica

-

purified enzyme, complete inactivation within 3 min

675101

80

Vigna unguiculata

-

enzyme is highly stable even 5 min at 80°C

706120

additional information

Fragaria x ananassa

-

biphasic thermal inactivation kinetics

675166

additional information

Lablab purpureus

-

the activated enzyme is less thermostable than the native enzyme, overview

671857

additional information

Ocimum basilicum

-

heat denaturation kinetics

674089

additional information

Prunus armeniaca

-

presence of a thermostable polyphenol oxidase forming and destabilizing mechanism in apricot. The loss of PPO thermostability by partial purification indicates the non-covalent nature of the stabilization

658950

additional information

Vitis vinifera

-

inactivation of the enzyme in freshly prepared grape must under high hydrostatic pressure of 100-800 MPa, combined with moderate temperature (20-70°C), or atmospheric pressure conditions in a temperature range of 55-70°C, pressure and temperature act synergistically, except in the hightemperature-low-pressure region where an antagonistic effect is found, overview, thermal inactivation of PPO in a biphasic model

674096