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2,4,6-Trinitrobenzenesulfonate
-
0.5 M, pH 9.0, 25°C, native wild-type enzyme: half-life 3.5 min, recombinant wild-type enzyme: half-life: 5.1 min, recombinant mutant H30A: half-life 5.5 min, recombinant mutant K170R half-life 101 min
4-chloromercuribenzoate
-
26.6% inhibition at 1 mM
5,5'-dithiobis(2-nitrobenzoate)
-
Mn-SOD
5-(((2,4-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3,5-dichlorobenzyl)(ethyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3,5-dichlorobenzyl)(isopropyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3,5-dichlorobenzyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3,5-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3,5-dichlorophenethyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-(((3-(3,5-dichlorophenyl)propyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-((benzyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-((cyclopropyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
5-[(3,5-dichlorophenoxy)methyl]-1,2-dihydro-3H-pyrazol-3-one
5-[[(3,5-dichlorophenyl)(methyl)amino]methyl]-1,2-dihydro-3H-pyrazol-3-one
beta-naphthoquinone-4-sulfonic acid
-
-
chloroform:ethanol solution
the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution
concanavalin A
-
inhibition in vivo and in vitro, essentially dependent on calcium chloride
-
di-N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium sulfate
diethyl dicarbonate
-
at 2.5 mM
Dithiocarbamate
strongly inhibits the activity of the rOf-cCu/ZnSOD
DTPA
-
i.e. diethylenetriamine-N,N,N,N,N-pentaacetic acid, inhibits the reductive decomposition of S-nitroso-L-glutathione catalyzed by superoxide dismutase by binding to the solvent-exposed active-site copper of one subunit without removing it. The resulting conformational change at the second active site inhibits the S-nitroso-L-glutathione reductase but not superoxide dismutase activity
DTT
13% inhibition at 2.5 mM
guanidine hydrochloride
-
Guanidinium chloride
-
Mn-SOD, 70% inhibition at 1 mM
guanidinium hydrochloride
HgCl2
-
1 mM, inhibition of isozyme SODI
HS-
substrate analogue, formation of a green complex upon binding
iodoacetic acid
-
23.5% inhibition at 1 mM
Mn(Me-Phimp)2(ClO4)
-
i.e. Mn(2-(1-(2-phenyl-2-(pyridine-2-yl)hydrazono)ethyl)phenol)chlorate, active as cofactor in superoxide dismutation reaction
Mn(N-Phimp)2
-
i.e. Mn-(2-((2-phenyl-2-(pyridin-2-yl)hydrazono)methyl)naphthalen-1-ol), active as cofactor in superoxide dismutation reaction
Mn(N-Phimp)2(ClO4)
-
i.e. Mn(2-((2-phenyl-2-(pyridin-2-yl)hydrazono)methyl)naphthalen-1-ol)chlorate, active as cofactor in superoxide dismutation reaction
Mn(Phimp)2
-
i.e. Mn(2-((2-phenyl-2-(pyridin-2-yl)hydazono)methyl)phenol), active as cofactor in superoxide dismutation reaction
Mn(Phimp)2(ClO4)
-
i.e. Mn(2-((2-phenyl-2-(pyridin-2-yl)hydazono)methyl)phenol)chlorate, active as cofactor in superoxide dismutation reaction
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium chloride
-
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium citrate
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium dibasic phosphate
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium L-tartrate
N-ethyl-5-phenylisoxazolium 3'-sulfonate
-
i.e. Woodwards reagent, Kat 50 mM
O2-
-
substrate inhibition for mutant C140S/Q143A
OH-
-
Cu,Zn-SOD, competitively
para-diazobenzene sulfonic acid
-
-
Pectin
-
from avocado root or cell wall
Penicillamine
-
copper-chelator, wild-type and mutant Cu,Zn-SOD
perchlorate
-
competitive
phenyl mercuric acetate
-
Cu,Zn-SOD
Phenylglyoxal
-
25% activity remaining after 3 h for native and recombinant wild-type and recombinant mutant H30A, complete inactivation of recombinant mutant K179R after 7 min
phenylmethanesulfonyl fluoride
irreversible inactivation by attachment of a molecule phenylmethanesulfonyl fluoride to the active site Tyr41 reinforcing the heat stability of the enzyme, overview
phenylmethylsulfonyl fluoride
phosphate
-
100 mM, 50% inhibition
polygalacturonase
-
from avocado root or cell wall
-
Sodium diethyldithiocarbamate
-
complete inhibition above 0.1 mM
Sodium fluoride
inhibits both the Mn- and Fe-reconstituted enzyme. The concentrations of sodium fluoride causing 50% inhibition of the Mn- and Fe reconstituted enzymes are 89 and 13 mM, respectively
tetrathiomolybdate
-
i.e. ATN-224, choline salt, inhibition leads to antiangiogenic and antitumour effects
-
trichloromethane-ethanol
-
-
2-mercaptoethanol
-
25% inhibition of Mn-reconstituted wild-type enzyme at 10 mM, no inhibition at 1 mM
2-mercaptoethanol
17% inhibition at 3 mM
2-mercaptoethanol
gradual inhibition by increasing concentration of 2-mercaptoethanol, 20% inhibition at 2 mM, 36% at 16 mM
2-mercaptoethanol
8 mM, slight inhibition
2-mercaptoethanol
-
15% inhibition
5-(((2,4-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 630 nM
5-(((2,4-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 630 nM
5-(((3,5-dichlorobenzyl)(ethyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 420 nM
5-(((3,5-dichlorobenzyl)(ethyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 420 nM
5-(((3,5-dichlorobenzyl)(isopropyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00108 mM
5-(((3,5-dichlorobenzyl)(isopropyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00108 mM
5-(((3,5-dichlorobenzyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 480 nM
5-(((3,5-dichlorobenzyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 480 nM
5-(((3,5-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 470 nM
5-(((3,5-dichlorobenzyl)(propyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 470 nM
5-(((3,5-dichlorophenethyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00164 mM
5-(((3,5-dichlorophenethyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00164 mM
5-(((3-(3,5-dichlorophenyl)propyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00332 mM
5-(((3-(3,5-dichlorophenyl)propyl)(methyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00332 mM
5-((benzyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00245 mM
5-((benzyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00245 mM
5-((cyclopropyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00134 mM
5-((cyclopropyl(3,5-dichlorobenzyl)amino)methyl)-1H-pyrazol-3(2H)-one
-
EC50 value is 0.00134 mM
5-[(3,5-dichlorophenoxy)methyl]-1,2-dihydro-3H-pyrazol-3-one
-
EC50 value is 400 nM
5-[(3,5-dichlorophenoxy)methyl]-1,2-dihydro-3H-pyrazol-3-one
-
EC50 value is 400 nM
5-[[(3,5-dichlorophenyl)(methyl)amino]methyl]-1,2-dihydro-3H-pyrazol-3-one
-
EC50 value is 570 nM
5-[[(3,5-dichlorophenyl)(methyl)amino]methyl]-1,2-dihydro-3H-pyrazol-3-one
-
EC50 value is 570 nM
azide
the azide ion acts as a strong competitive inhibitor for SOD by binding directly to the active site metal. Azide is bound end-on at the sixth coordinate position of the manganese ion. Tetrameric electrostatic surfaces are calculated incorporating accurate partial charges for the active site in three states, including a state with superoxide coordinated to the metal using the position of azide as a model
azide
-
causes 50% inhibition at 20 mM
azide
Mn2+-reconstituted recombinant enzyme and Fe2+-reconstututed recombinant enzyme displays relatively strong resistance against azide. Mn2+- and Fe2+-reconstituted activity decreases 50% with 380 and 340 mM azide, respectively
Ba2+
inhibitory at 1 mM
Ca2+
inhibitory at 1 mM
chloride
-
-
chloride
-
100 mM, 50% inhibition
chloroform
-
Mn-SOD
chloroform
recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture
CN-
-
-
CN-
slight inhibition, Mn-SOD
CN-
-
no inhibition Mn-SOD
CN-
-
at 1-3 mM, complete inhibition; Cu,Zn-SOD
CN-
-
slight inhibition, Mn-SOD
CN-
-
extracellular enzyme
CN-
Megalodesulfovibrio gigas
-
no inhibition: Fe-SOD
CN-
-
contains a cyanide-sensitive enzyme in cytosol and mitochondrial intermembrane space and one cyanide-insensitive enzyme in mitochondrial matrix; Cu,Zn-SOD; no inhibition Mn-SOD
CN-
-
no inhibition Mn-SOD
CN-
-
no inhibition Mn-SOD
CN-
-
Cu,Zn-SOD; no inhibition Mn-SOD
CN-
-
SOD-2 and SOD-4 inhibited, SOD-3 not inhibited
Co2+
inhibitory at 1 mM
Co2+
-
inhibits by 25% at 10 mM
Cu2+
50% inhibition at 1 mM
Cu2+
-
slight inhibition at 1.0-5.0 mM
cyanide
-
the isoenzyme Cu/Zn-SOD is cyanide-sensitive, while the Mn-SOD is not
cyanide
Radix lethospermi
-
-
di-N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium sulfate
-
EC50 value is 480 nM
di-N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium sulfate
-
EC50 value is 480 nM
diethyldithiocarbamate
-
-
diethyldithiocarbamate
complete inhibition, Cu,Zn-SOD
diethyldithiocarbamate
-
-
diethyldithiocarbamate
-
-
diethyldithiocarbamate
-
-
diethyldithiocarbamate
-
Mn-SOD; slightly
diethyldithiocarbamate
a specific inhibitor for CuZn-SODs
diethyldithiocarbamate
-
strong inhibition
diethyldithiocarbamate
-
strong inhibition, extracellular enzyme
diethyldithiocarbamate
-
copper-chelator, wild-type and mutant Cu,Zn-SOD
diethyldithiocarbamate
-
inhibits recombinant Cu,Zn-SOD, at 0.05-0.1 mM inactivation occurs gradually within 1 h
diethyldithiocarbamate
-
slightly
diethyldithiocarbamate
complete inhibition at 1.4 mM, a specific inhibitor for CuZn-SODs
diethyldithiocarbamate
-
causes decline of the enzyme in various tissues after intraperitoneal injection, alpha-tocopherol feeding prior to application of diethyldithiocarbamate leads to reduced inhibition of the enzyme
diethyldithiocarbamate
-
Cu,Zn-SOD
diethyldithiocarbamate
-
40% inhibition
diethyldithiocarbamate
inhibits SodC specifically
EDTA
-
60% inhibition of Mn-reconstituted wild-type enzyme at 10 mM, 20% at 1 mM
EDTA
-
i.e. diethylenediamine-N,N,N,N-tetraacetic acid, inhibits the reductive decomposition of S-nitroso-L-glutathione catalyzed by superoxide dismutase by binding to the solvent-exposed active-site copper of one subunit without removing it. The resulting conformational change at the second active site inhibits the S-nitroso-L-glutathione reductase but not superoxide dismutase activity
EDTA
15% inhibition at 0.5 mM
EDTA
-
inhibition is reversible by Cu and Zn
EDTA
strong inhibition at 10 mM
EDTA
gradual inhibition by increasing concentration of EDTA
EDTA
-
66% inhibition at 1 mM
EDTA
Radix lethospermi
-
2 mM, 18% inhibition
ethanol
-
Mn-SOD
ethanol
recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture
Fe2+
inhibitory at 1 mM
Fe2+
-
inhibits by 44% at 10 mM
fluoride
-
Fe-SOD
fluoride
recombinant Fe-reconstituted SOD
guanidinium hydrochloride
-
25% inhibition of Mn-reconstituted wild-type enzyme at 10 mM
guanidinium hydrochloride
40% inhibition at 2 M, 85% at 5 M
guanidinium hydrochloride
69% inhibition at 6 M
guanidinium hydrochloride
6 M, slight inhibition
guanidinium hydrochloride
-
up to 50% inhibition
H2O2
-
no inhibition: Mn-SOD
H2O2
inactivates the Fe-reconstituted SOD in a time-dependent manner, but not the Mn-reconstituted enzyme. The incubation time for 50% inactivation of the Fe-reconstituted enzyme in the presence of 0.24 mM H2O2 is 50 min
H2O2
complete inhibition; Cu,Zn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
50% inhibition at 4.3 mM
H2O2
-
no inhibition: Mn-SOD
H2O2
-
no inhibition: Mn-SOD
H2O2
2.5 mM, 20% residual activity
H2O2
inactivation of FeSOD; inactivation of FeSOD; inactivation of FeSOD
H2O2
58% inhibition at 2 mM
H2O2
-
partial sensitivity
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
extracellular enzyme, rapidly
H2O2
gradual inhibition by increasing concentration of H2O2, 36% inhibition at 2 mM, 81% at 16 mM
H2O2
-
10 mM, complete inhibition
H2O2
Megalodesulfovibrio gigas
-
-
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
no inhibition: Fe-SOD
H2O2
-
H2O2 does not show significant inhibition of the SOD activity in cell-free extracts prepared from cells grown in Mn-rich medium, but inhibits 30% of the enzyme activity in cell extracts from cells grown in Fe-rich medium
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
no inhibition: Mn-SOD
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
50 mM, 100% inhibition
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
-
no inhibition: Mn-SOD
H2O2
addition of 5 mM of hydrogen peroxide (H2O2) completely inhibits of the enzyme
H2O2
-
no inhibition: Mn-SOD
H2O2
half-life of 22 min both in absence or presence of 2-mercaptoethanol
H2O2
preincubation in 5 mM H2O2 for 30 min results in a loss of 91% activity
H2O2
-
no inhibition: Mn-SOD
H2O2
Radix lethospermi
-
-
H2O2
-
inhibition at 10 mM, the Fe-SOD is less sensitive
H2O2
distinguishes Fe-SOD from Mn-SOD, since it inactivates only Fe-SOD
H2O2
-
90% inhibition at 5 mM
H2O2
-
no inhibition: Fe-SOD; no inhibition: Mn-SOD
H2O2
-
no inhibition: Mn-SOD
H2O2
-
inactivation kinetics of rSmSOD by hydrogen peroxide, overview
H2O2
Thermochaetoides thermophila
-
H2O2
-
Cu,Zn-SOD; no inhibition: Mn-SOD
H2O2
5 mM, partial inhibition
H2O2
-
no inhibition: Mn-SOD
H2O2
-
SOD-2 and SOD-4 inhibited, SOD-3 not
Hg2+
-
strong inhibition
Hg2+
-
35% inhibition at 0.1 mM, complete inhibition at 5 mM
Hg2+
-
inhibits by 35% at 10 mM
hydrogen peroxide
-
hydrogen peroxide
-
up to 80% inhibition
imidazole
above 1.6 mM
imidazole
1 mM, slight inhibition
Iodine
-
-
Iodine
-
completely inhibits the cell wall SOD, the inhibition is partly, up to 70%, reversed by 2-mercaptoethanol
iodoacetamide
-
Cu,Zn-SOD
iodoacetamide
-
2 mM, 15% inhibition
K+
-
inhibits by 14% at 10 mM
KCN
-
KCN
5 mM, 40% residual activity
KCN
-
30% inhibition at 2 mM
KCN
3 mM, 65% loss of activity
KCN
strongly inhibits the activity of the rOf-cCu/ZnSOD
KCN
complete inhibition at 0.16 mM, a specific inhibitor for CuZn-SODs
KCN
-
inhibition at 10 mM, the Fe-SOD is less sensitive
KCN
-
complete inhibition at 5 mM
KCN
Thermochaetoides thermophila
-
KCN
2 mM, complete inhibition
Mg2+
inhibitory at 1 mM
Mg2+
30% inhibition at 1 mM
Mg2+
-
25% inhibition at 5 mM
Mg2+
-
inhibits by 64% at 10 mM
Mn2+
-
55% inhibition
Mn2+
-
35% inhibition at 5 mM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium chloride
-
EC50 value is 510 nM
-
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium chloride
-
EC50 value is 510 nM
-
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium citrate
-
EC50 value is 480 nM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium citrate
-
EC50 value is 480 nM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium dibasic phosphate
-
EC50 value is 480 nM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium dibasic phosphate
-
EC50 value is 480 nM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium L-tartrate
-
EC50 value is 480 nM
N-(3,5-dichlorobenzyl)-N-methyl-1-(5-oxo-2,5-dihydro-1H-pyrazol-3-yl)methanaminium L-tartrate
-
EC50 value is 480 nM
N3-
-
-
N3-
-
Mn-SOD is inhibited by 50%, enzyme reconstituted by Fe3+ shows increased inhibition
N3-
-
extracellular enzyme; Mn-SOD
N3-
-
binds to Fe3+, but has no effect on activity
N3-
-
no inhibition, Fe-SOD
N3-
-
extracellular enzyme
N3-
Megalodesulfovibrio gigas
-
-
N3-
-
no inhibition, Fe-SOD
N3-
-
no inhibition: Zn,Cu-SOD
N3-
-
SOD-2 and SOD-4 inhibited, SOD-3 not inhibited
NaCl
-
slight inhibition at 1 mM
NaCl
60% activity remains at 2.5 M NaCl
NaCl
activity of SaCSD1 decreases with the increase of salt concentration
NaN3
-
13.5 mM, inhibition
NaN3
-
inactivates 15% and 24% of the SODs present in cell extracts prepared from cells grown in the Mn- and Fe-rich media, respectively
NaN3
low inhibition by sodium azide, 40% inhibition at 50 mM
NaN3
50% inhibition at 6.9 mM in absence, at 9.0 mM in presence of 2-mercaptoethanol
NaN3
-
inhibition at 5 mM, the Fe-SOD is less sensitive
NaN3
-
inhibits the enzyme mutants Y41F and H155Q, but not the wild-type enzyme
NaN3
Thermochaetoides thermophila
-
-
Ni2+
inhibitory at 1 mM
Ni2+
inhibits slightly at 1 mM
Ni2+
-
inhibits by 57% at 10 mM
o-phenanthroline
-
-
o-phenanthroline
-
Mn-SOD
o-phenanthroline
-
slightly
o-phenanthroline
-
depending on assay method; Fe-SOD
p-hydroxymercuribenzoate
-
completely inhibited at 1 mM; Mn-SOD
p-hydroxymercuribenzoate
-
completely inhibited at 1 mM; Mn-SOD
peroxynitrite
almost complete inhibition via nitration of active-site residue Y34, no significant change in conformation upon nitration. Inhibition occurs either through a steric effect of 3-nitrotyrosine 34 that impedes substrate binding or through an electrostatic effect of the nitro group
peroxynitrite
50% inhibition at 0.032 mM in absence, at 0.153 mM in presence of 2-mercaptoethanol
phenylmethylsulfonyl fluoride
-
phenylmethylsulfonyl fluoride
-
2 mM, 15% inhibition
PMSF
-
PMSF
-
irreversible inhibition by binding to active site Tyr41
potassium cyanide
-
2 mM, complete inhibition
potassium cyanide
-
up to 90% inhibition
SDS
-
60% inhibition of Mn-reconstituted wild-type enzyme at 1%, and 40% inhibition of the Mn-reconstituted N-terminal domain
SDS
-
recombinant Cp-icCuZnSOD is active and retains more than 80% activity under treatment with 1-6% SDS. It retains 70% activity after treatment with 8% SDS but activity is rapidly lowered to 52% after treatment with 10% SDS
SDS
-
0.5 mM, complete inhibition
SDS
-
19% inhibition at 0.1 mM, complete inhibition at 0.5 mM
SDS
-
1 mM, inhibition of isozyme SODI
Sodium azide
50% inhibition of the Fe-reconstituted enzyme at 41 mM. Sodium azide does not inhibit the Mn-reconstituted superoxide dismutase even at concentrations up to 400 mM
Sodium azide
-
33% inhibition
Sodium azide
-
32% inhibition
Sodium cyanide
-
-
Sodium cyanide
-
36% inhibition
Sodium dodecyl sulfate
-
1%, complete inhibition
Sodium dodecyl sulfate
-
2% w/v, Cu,Zn-SOD and EC-SOD
Sodium dodecyl sulfate
-
-
sodium dodecylsulfate
-
up to 67% inhibition
sodium dodecylsulfate
Radix lethospermi
-
2 mM, 34% inhibition
Triton X-100
-
Triton X-100
70% inhibition
Urea
-
Mn-SOD, 90% inhibition at 6 M
Urea
8 M, slight inhibition
Zn2+
inhibitory at 1 mM
Zn2+
-
strong inhibition at 1.0-5.0 mM
Zn2+
-
12% inhibition at 5 mM
ZnCl2
-
slight inhibition at 1 mM
additional information
no inhibition by hydrogen peroxide or potassium cyanide
-
additional information
-
no inhibition by hydrogen peroxide or potassium cyanide
-
additional information
no inhibition by KCN of MnSOD
-
additional information
no inhibition by KCN of MnSOD
-
additional information
-
enzyme is not inhibited by H2O2 and unusually resistant to KCN; SodC is resistant to inhibition by H2O2 and is unusually resistant to KCN for a Cu,Zn-SOD
-
additional information
no inhibition by sodium azide or potassium cyanide
-
additional information
-
no inhibition by sodium azide or potassium cyanide
-
additional information
the enzyme is resistant to denaturation by sodium dodecyl sulfate (SDS) and urea
-
additional information
no inhibition by 10 mM NaN3
-
additional information
-
no inhibition by 10 mM NaN3
-
additional information
not inhibitory
H2O2 up to 80 mM
-
additional information
-
not inhibitory
H2O2 up to 80 mM
-
additional information
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not inhibitory: NaN3
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additional information
not inhibitory: NaN3
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additional information
not inhibitory: sodium dodecylsulfate
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additional information
imidazole up to 0.8 M and sodium dodecylsulfate up to 4% are not inhibitory
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additional information
no inhibition by CN; no inhibition by CN; no inhibition by CN
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additional information
no inhibition by CN; no inhibition by CN; no inhibition by CN
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additional information
no inhibition by CN; no inhibition by CN; no inhibition by CN
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additional information
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no inhibition by CN; no inhibition by CN; no inhibition by CN
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additional information
no inhibition by NaN3 and KCN
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additional information
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no inhibition by NaN3 and KCN
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additional information
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UV-B radiation decreases the SOD activity
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additional information
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not inhibitory: sodium dodecyl sulfate up to 4%
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additional information
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not inhibitory: cyanide
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additional information
not inhibitory: sodium dodecylsulfate up to 2.5%
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additional information
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not inhibitory: sodium dodecylsulfate up to 2.5%
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additional information
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no inhibition by dithiothreitol and beta-mercaptoethanol
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additional information
insensitivity of the recombinant enzyme to both KCN and H2O2
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additional information
the enzyme shows good tolerance to some inhibitors, detergents, and denaturants
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additional information
insensitivity of the recombinant enzyme to both KCN and H2O2. The enzyme shows a relatively good tolerance to some inhibitors, detergents, and denaturants, such as 2-mercaptoethanol, dithiothreitol, phenylmethylsulfonyl fluoride, Chaps, Triton X-100, urea, and guanidine hydrochloride
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additional information
tributyltin chloride does not affect enzyme expression
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additional information
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tributyltin chloride does not affect enzyme expression
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additional information
the conserved, active-site residue Tyr34 mediates product inhibition
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additional information
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the conserved, active-site residue Tyr34 mediates product inhibition
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additional information
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ligand complex synthesis, electrochemical properties, and structure determination, overview
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additional information
NO-induced damage in eSOD causes alteration in hydrophobic or aromatic amino acids and protein carbonyl contents
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additional information
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NO-induced damage in eSOD causes alteration in hydrophobic or aromatic amino acids and protein carbonyl contents
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additional information
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pyrazolone derivatives are inhibitors of Cu/Zn superoxide dismutase 1 (SOD1)-dependent protein aggregation and are useful as drugs in treatment of amyotrophic lateral sclerosis (ALS). Design and synthesis of a series of tertiary amine-containing pyrazolones and their structure-activity relationships, conjugate salts greatly improved their solubility, overview
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additional information
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insensitive to fluoride
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additional information
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no inhibition of Cu/ZnSOD by 10 mM H2O2, 1 mM CaCl2, and 1 mM NaN3
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additional information
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not inhibitory: cyanide
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additional information
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not inhibitory: cyanide
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additional information
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not inhibitory: KCN
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additional information
the enzyme is not inhibited by cyanide (10 mM)
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additional information
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the enzyme is not inhibited by cyanide (10 mM)
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additional information
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compound 2-[(3-iodophenyl)methylsulfanyl]-5-pyridin-4-yl-1,3,4-oxadiazole, a known protein kinase inhibitor, decreases enzyme mutant G93A-SOD1 expression in vitro and in the brain and spinal cord in vivo, but this compound has a biphasic dose response curve and a likely toxophore which limit its therapeutic window for chronic disease such as amyotrophic lateral sclerosis (ALS). Therefore, a focused library of analogues are tested for the ability to decrease SOD1 expression in vitro. This exercise results in the identification of a lead compound with improved drug-like characteristics and activity. Development of small molecules that reduce the expression of etiologically relevant toxic proteins, structureactivity relationships, overview. Compounds 3-[1-(3-hydroxypropyl)-1H-pyrrolo[2,3-b]pyridin-3-yl]-4-(pyrazin-2-yl)-1H-pyrrole-2,5-dione, 2-chloro-1-(4,5-dibromothiophen-2-yl)ethan-1-one, 2-bromo-1-(4-bromophenyl)ethan-1-one, 4-(5-[[(3-iodophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(3-methoxyphenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(3-fluorophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-[5-([[4-(trifluoromethyl)phenyl]methyl]sulfanyl)-1,3,4-oxadiazol-2-yl]pyridine, 4-(5-[[(3-chlorophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(3-nitrophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(3-bromophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-methoxyphenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-chlorophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-bromophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-iodophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-fluorophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-methylphenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-nitrophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine, 4-(5-[[(4-nitrophenyl)methyl]sulfanyl]-1,3,4-oxadiazol-2-yl)pyridine suppress the enzyme expression
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additional information
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pyrazolone derivatives are inhibitors of Cu/Zn superoxide dismutase 1 (SOD1)-dependent protein aggregation and toxicity, and are useful as drugs in treatment of amyotrophic lateral sclerosis (ALS). Design and synthesis of a series of tertiary amine-containing pyrazolones and their structure-activity relationships, conjugate salts greatly improve their solubility, overview. Identification of several neuron-protection scaffolds based on mitigating protein aggregation and toxicity. A tertiary amine fragment is incorporated into the linker between the pyrazolone and the aryl group on the basis of principles of optimal brain exposure in CNS drug molecule design, the tertiary amine pyrazolone scaffolds exhibited superior properties for potential neuronal activity and in metabolic studies, such as microsomal stability, plasma stability, and Caco-2 permeability
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additional information
poor inhibition by KCN, no inhibition by EDTA and cuprizone
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additional information
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poor inhibition by KCN, no inhibition by EDTA and cuprizone
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additional information
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not inhibitory: sodium azide, potassium cyanide
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additional information
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not inhibitory: EDTA
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additional information
no inhibition by EDTA and poor inhibition by NaN3
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additional information
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no inhibition by EDTA and poor inhibition by NaN3
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additional information
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not inhibitory: cyanide, hydrogen peroxide, ditiothreitol, sodium azide, Triton X-100, and 2-mercaptoethanol
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additional information
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not inhibitory: cyanide
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additional information
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no inhibition by KCN or H2O2. Level of activity of the MnSOD polypeptides decreases in the presence of avocado root or cell wall components, addition of avocado root, pectin, or polygalacturonase to the incubation medium results in a significant increase in the accumulation of O2.-
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additional information
not inhibitory: sodium dodecylsulfate
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additional information
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not inhibitory: sodium dodecylsulfate
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additional information
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cyanide at 5 mM and H2O2 at 3 mM have no effect on the activity of the enzyme
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additional information
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no inhibition by N-ethylmaleide
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additional information
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no effect: Cu2+, Co2+, Ca2+, sodium dodecylsulfate, 2-mercaptoethanol
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additional information
Radix lethospermi
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not inhibitory: dithiothreitol, sodium azide, 2-mercaptoethanol
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additional information
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no effect on the enzyme by alpha-tocopherol in absence of diethyldithiocarbamate
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additional information
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melatonin, testosterone, dihydrotestosterone, estradiol, and vitamin D induce activation of SOD1 in mitochondria. Enzyme activation is not affected by furafylline, a selective inhibitor of the P450 1A2 isoform, but is inhibited by omeprazole and ketoconazole, and by tiron, a superoxide radical specific scavenger
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additional information
no inhibition by DTT. Ba2+, Ca2+, Ni2+, and Fe2+ have no obvious impact to enzyme activity at 1 mM
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additional information
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no inhibition by DTT. Ba2+, Ca2+, Ni2+, and Fe2+ have no obvious impact to enzyme activity at 1 mM
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additional information
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the enzyme is insensitive to cyanide inhibition
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additional information
recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site
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additional information
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recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site
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additional information
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no inhibition of Cu,Zn-SOD by sodium azide and O-phenanthroline
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additional information
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no effect: Cu2+, Ca2+
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additional information
the SaCSD1 protein is very susceptive to pepsin digestion
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additional information
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the SaCSD1 protein is very susceptive to pepsin digestion
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additional information
the enzyme is insensitive to malondialdehyde (MDA) or 4-hydroxy-2-nonenal (HNE); the enzyme is insensitive to malondialdehyde (MDA) or 4-hydroxy-2-nonenal (HNE). Enzyme Mn-SOD is insensitive to cyanide
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additional information
the enzyme is insensitive to malondialdehyde (MDA) or 4-hydroxy-2-nonenal (HNE); the enzyme is insensitive to malondialdehyde (MDA) or 4-hydroxy-2-nonenal (HNE). Enzyme Mn-SOD is insensitive to cyanide
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additional information
no inhibition by NaN3
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additional information
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no inhibition by NaN3
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additional information
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no inhibition of the purified enzyme by 6 M guanidinium chloride or by proteases trypsin and Staphylococcus aureus V8 protease
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additional information
no inhibition of the purified enzyme by 6 M guanidinium chloride or by proteases trypsin and Staphylococcus aureus V8 protease
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additional information
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the native enzyme is degraded by pepsin and trypsin, while the polysialylated SOD is resistant to pepsin and trypsin
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additional information
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urea and iodoacetamide do not affect the enzymatic activity
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additional information
no inhibition by KCN and H2O2
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additional information
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no inhibition by KCN and H2O2
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additional information
Thermochaetoides thermophila
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no inhibition by KCN and H2O2
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additional information
Thermochaetoides thermophila
no inhibition by NaN3
-
additional information
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not inhibitory: potassium cyanide, H2O2
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additional information
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isoyzme SODI is insensitive to H2O2 and KCN
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additional information
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no inhiibtion by CN-
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additional information
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the enzyme is insensitive to potassium cyanide
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additional information
the purified recombinant enzyme shows a high degree of resistance to detergent, ethanol and protease digestion
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additional information
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the purified recombinant enzyme shows a high degree of resistance to detergent, ethanol and protease digestion
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additional information
no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate
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additional information
no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate
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additional information
no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate
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additional information
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no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate
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