2.2.1.7: 1-deoxy-D-xylulose-5-phosphate synthase

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For detailed information about 1-deoxy-D-xylulose-5-phosphate synthase, go to the full flat file.

Word Map on EC 2.2.1.7

2.2.1.7

isoprenoids

carotenoid

reductoisomerase

methylerythritol

isopentenyl

terpenoids

phytoene

plastidial

2-c-methyl-d-erythritol

dimethylallyl

2-c-methyl-d-erythritol-4-phosphate

non-mevalonate

fosmidomycin

thdp-dependent

isoprene

apocarotenoids

ggpps

diphosphate-dependent

clomazone

4-diphosphate

mevalonate-independent

analysis

drug development

medicine

synthesis

agriculture

biotechnology

industry

Reaction

Synonyms

1-D-deoxy-D-threo-2-pentulose 5-phosphate synthetase, 1-D-deoxyxylulose 5-phosphate synthase, 1-deoxy-D-threo-pentulose synthase, 1-deoxy-D-xylulose 5-phosphate synthase, 1-deoxy-D-xylulose 5-phosphate synthase 1, 1-deoxy-D-xylulose 5-phosphate synthase 2, 1-deoxy-D-xylulose 5-phosphate synthetase, 1-deoxy-D-xylulose-5-phosphate pyruvate lyase, 1-deoxy-D-xylulose-5-phosphate synthase, 1-deoxy-D-xylulose-5-phosphate synthase 1, 1-deoxy-D-xylulose-5-phosphate synthetase, 1-desoxy-D-xylulose-5-phosphate synthase, CSDXS, D-1-deoxyxylulose 5-phosphate synthase, deoxyxylulose 5-phosphate synthase, deoxyxylulose-5-phosphate synthase, deoxyxylulose-5-phosphate synthetase, DXP, DXP synthase, DXP-synthase, DXPase, DXPS, DXS, DXS-I, DXS-II, DXS1, dxs11, DXS2, DXS3, EC 4.1.3.37, GbDXS1, GbDXS2, glyceraldehydes 3-phosphate-pyruvate ligase, GmDXS1, MtDXS1, MtDXS2, OsDXS1, OsDXS2, OsDXS3, PaDXS1, PaDXS2A, PaDXS2B, PdDXS1, PdDXS2, synthase, 1-deoxy-D-xylulose 5-phosphate

ECTree

2 Transferases

2.2 Transferring aldehyde or ketonic groups

2.2.1 Transketolases and transaldolases

2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase

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Results	in table
3	Activating Compound
42134	AA Sequence
11	Application
1	CAS Registry Number
49	Cloned(Commentary)
29	Cofactor
3	Crystallization (Commentary)
14	Expression
23	General Information
48	IC50 Value
118	Inhibitors
64	kcat/KM [mM/s]
72	Ki Value [mM]
109	KM Value [mM]
20	Localization
22	Metals/Ions
33	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
135	Organism
13	Pathway
21	PDB ID
12	pH Optimum
1	pH Range
13	pI Value
2	Posttranslational Modification
45	Protein Variants
17	Purification (Commentary)
5	Reaction
2	Reaction Type
91	Reference
89	Source Tissue
6	Specific Activity [micromol/min/mg]
2	Storage Stability
114	Substrates and Products (Substrate)
30	Subunits
158	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
67	Turnover Number [1/s]

Purification

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Purification on EC 2.2.1.7 - 1-deoxy-D-xylulose-5-phosphate synthase

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3 entries

95% pure by SDS-PAGE

Mycobacterium tuberculosis

702548

ammonium sulfate precipitation and Ni-NTA column chromatography

Populus trichocarpa

736415

Cosmogel His-Accept column chromatography

Synechocystis sp.

P73067

736523

functional soluble, recombinant enzyme from Escherichia coli

Agrobacterium tumefaciens

Q5QKF8

674982

histidine-tagged recombinant protein, expressed in Escherichia coli

Pseudomonas aeruginosa

286425

histidine-tagged recombinant proteins DXP-synthase A and DXP-synthase B, expressed in Escherichia coli

Rhodobacter capsulatus

395816

Ni-NTA agarose column chromatography, amylose resin chromatography, and glutathione Sepharose column chromatography

Nicotiana tabacum

D2CS41

736967

Ni-NTA column chromatography and Superdex gel filtration

2 entries

Ni-NTA resin column chromatography

Deinococcus radiodurans

overexpression

recombinant His-tagged enzymes from Escherichia coli by nickel affinity and anion exchange chromatography, and gel filtration

2 entries

recombinant protein, expressed in Escherichia coli

Streptomyces sp.

395812