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2.4.1.174: glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

This is an abbreviated version!
For detailed information about glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.174

Reaction

UDP-N-acetyl-alpha-D-galactosamine
+
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
=
UDP
 +
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine

Synonyms

acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-chondroitin, I, ChGn-1, ChGn-2, chondroitin beta1,4-N-acetylgalactosaminyltransferase-1, chondroitin galactosaminyltransferase, chondroitin N-acetylgalactosaminyltransferase-1, chondroitin N-acetylgalactosaminyltransferase-2, chondroitin sulfate beta-1,4-N-acetylgalactosaminyltransferase-1 ChGn-1, chondroitin sulfate N-acetylgalactosaminyltransferase 1, chondroitin sulfate N-acetylgalactosaminyltransferase 2, chondroitin sulfate N-acetylgalactosaminyltransferase-1, chondroitin sulfate N-acetylgalactosaminyltransferase-2, chondroitin sulfate synthase 1, chondroitin sulfate synthase-1, chondroitin sulphate N-acetylgalactosaminyl-transferase-1, chondroitin sulphate N-acetylgalactosaminyltransferase 1, CHSY1, CS N-acetylgalactosaminyltransferase 1, CS N-acetylgalactosaminyltransferase-1, CSGal-NACT-1, CSGalNAcT-1, CSGalNAcT1, CSGALNACT2, CSS1, GalNAcT-I, GalNAcT-II, glucuronylgalactosylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase, More, N-acetylgalactosamineT-II, N-acetylgalactosaminyltransferase I, uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferase I

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.174 glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

Cloned

Cloned on EC 2.4.1.174 - glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CSGalNAcT1 expression in C57BL/6 ES cells
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CSS1 quantitative expression analysis by realtime RT-PCR, overview
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DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein
DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium
expression of ChGn-1 in mutant sog9 L cells
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gene ChGn1, expression of His-tagged wild-type ChGn-1 and soluble mutant enzymes in COS-1 cells, secretion to the medium
gene Csgalnact1
gene CSGALNACT1 or t1, quantitative RT-PCR enzyme expression analysis of CS glycosyltransferases
gene csgalnact1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene CSGALNACT1, genotyping and polymorphisms, recombinant expression of His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant in COS-1 cells, the enzymes are secreted
gene CSGALNACT1, quantitative real-time PCR enzyme expression analysis
gene CSGALNACT1, quantitative real-time RT-PCR enzyme expression analysis
gene CSGalNAcT1, recombinant expression of wild-type and mutant FLAG-tagged CSGalNAcT-1 enzymes in COS-7 cells
gene CSGALNACT2 or t2, quantitative RT-PCR enzyme expression analysis of CS glycosyltransferases
gene CSGalNAcT2, cloning from DF-1 cells, overexpression in DF1 cells, semiquantitative real-time RT-PCR enzyme expression analysis, interaction analysis of enzyme CSGalNAcT2 with IBDV viral capsid protein VP2using the yeast two-hybridization assay
gene csgalnact2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene CSGALNACT2, quantitative real-time RT-PCR enzyme expression analysis
LTC cells from rat chondrosarcoma are transfected with chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression plasmid (about 80fold increase of chondroitin sulfate N-acetylgalactosaminyltransferase-1 RNA), metabolic albeling with [35S]sulfate show up to a 2.2fold increase in chondroitin sulfate levels in these cells, but the mRNA level of aggrecan core protein does not change. Aggrecan obained from chondroitin sulfate N-acetylgalactosaminyltransferase-1 overexpressing cells contain a larger amount of chondroitin sulfate, but the chondroitin sulfate chain length is similar to mock-transfected cells.
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Myc-tagged CSGalNAcT-1 expression in HeLa cells
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