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low content
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low content
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melanoma cell line
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very high content
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expression of Chsy1 is up-regulated in skeletal elements
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chondrosarcoma cell line
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thyroid cancer cell line
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low content
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low content
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myeloma cell line
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quantitative gene expression and in situ hybridization of genes associated with endochondral ossification
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quantitative gene expression and in situ hybridization of genes associated with endochondral ossification
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quantitative analysis of Csgalnact1 and Csgalnact2 transcripts in brain of wild-type and Csgalnact1-/- mice by real-time PCR, overview
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quantitative analysis of Csgalnact1 and Csgalnact2 transcripts in brain of wild-type and Csgalnact1-/- mice by real-time PCR, overview
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articular
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growth plate cartilage
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knee, quantitative RT-PCR enzyme expression analysis of CSGALNACT1
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knee, quantitative RT-PCR enzyme expression analysis of CSGALNACT2
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growth plate cartilage
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knee, quantitative RT-PCR enzyme expression analysis of CSGALNACT1
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knee, quantitative RT-PCR enzyme expression analysis of CSGALNACT2
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pectoral
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pectoral and median
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very low content
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peripheral blood
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peripheral blood
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low content
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very low content
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low content
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high content
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high content
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very high content
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additional information
gene csgalnact1 expression analysis during fish development, in situ immunohistochemic analysis, overview. Gene csgalnact1 mRNA is not maternally deposited, instead a rather weak and ubiquitous expression is seen during early somitogenesis. At 24 hr post fertilization, gene csgalnact1 expression is mostly restricted to the notochord and to the otic capsule, but weak staining is also seen in the intermediate cell mass, a hematopoietic region at this stage. At later stages, csgalnact1 expression becomes restricted mainly to cartilage structures, and the regions of the pharyngeal arches, the otic capsule, the pectoral fins, the notochord, and the median fin fold. At 72 hr post fertilization, weak staining in brain tissue and the spinal cord appears, while strong staining is detected in cartilage structures and the brain ventricular zone
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additional information
gene csgalnact1 expression analysis during fish development, in situ immunohistochemic analysis, overview. Gene csgalnact1 mRNA is not maternally deposited, instead a rather weak and ubiquitous expression is seen during early somitogenesis. At 24 hr post fertilization, gene csgalnact1 expression is mostly restricted to the notochord and to the otic capsule, but weak staining is also seen in the intermediate cell mass, a hematopoietic region at this stage. At later stages, csgalnact1 expression becomes restricted mainly to cartilage structures, and the regions of the pharyngeal arches, the otic capsule, the pectoral fins, the notochord, and the median fin fold. At 72 hr post fertilization, weak staining in brain tissue and the spinal cord appears, while strong staining is detected in cartilage structures and the brain ventricular zone
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additional information
gene csgalnact2 expression analysis during fish development, in situ immunohistochemic analysis, overview. The csgalnact2 probe strongly stains embryos already at the 2-cell stage indicating maternally deposited mRNA. Notochord staining is visible already from the 5-somite stage and expression is apparent at the 15-somite stage in the tail bud and in the notochord, whereas the expression at 24 hr post fertilization is restricted to the brain tissue and the posterior region of the somites and the notochord. Expression is further detected in brain tissue at 36 and 48 hr post fertilization when staining appeared also in the pectoral fins, the notochord, the otic capsule, and cartilage structures. By 72 hr post fertilization, expression is restricted to the head, the spinal cord, and the pronephric ducts
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additional information
gene csgalnact2 expression analysis during fish development, in situ immunohistochemic analysis, overview. The csgalnact2 probe strongly stains embryos already at the 2-cell stage indicating maternally deposited mRNA. Notochord staining is visible already from the 5-somite stage and expression is apparent at the 15-somite stage in the tail bud and in the notochord, whereas the expression at 24 hr post fertilization is restricted to the brain tissue and the posterior region of the somites and the notochord. Expression is further detected in brain tissue at 36 and 48 hr post fertilization when staining appeared also in the pectoral fins, the notochord, the otic capsule, and cartilage structures. By 72 hr post fertilization, expression is restricted to the head, the spinal cord, and the pronephric ducts
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additional information
immunohistochemic analysis of enzyme content and localization, overview
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isozymes CSGALNACT1 (T1) and CSGALNACT2 (T2) are expressed in different organs
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additional information
isozymes CSGALNACT1 (T1) and CSGALNACT2 (T2) are expressed in different organs
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additional information
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the enzyme is expressed (at very high levels) in the prehypertrophic zone of the growth plate at E16.5, colocalized with aggrecan core protein. In ATDC5 cells the expression of CSGlcAT and CSGalNAcT-1 correlate well with that of aggrecan core protein. Real time RT-PCR show a high level of chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression in cartilage at E18.5.
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CSS1 quantitative tissue expression analysis by realtime RT-PCR, overview
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additional information
isozymes CSGALNACT1 (T1) and CSGALNACT2 (T2) are expressed in different organs
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additional information
isozymes CSGALNACT1 (T1) and CSGALNACT2 (T2) are expressed in different organs
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